Moonlight receptor of the "1-h-midge"<i>Clunio marinus</i>studied by micro-XRF

Falkenberg, Gerald; Fleissner, Ge; Neumann, Dietrich; Wellenreuther, G; Alraun, Philipp; Fleissner, Gue

doi:10.1088/1742-6596/463/1/012016

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…The experimental animal of this project were larvae of the intertidal midge Clunio marinus, which were reared as synchronized mass cultures in the animal houses of the Zoological Institutes at Vienna University in isolated chambers filled with artificial seawater, at constant temperature and under a strict light regime [2]. The correlative LM -XRF study included 16 ocelli samples fixed at 7 different lunar cycle days [5,6]. One sample, which was fixed about a week after the start of the dark moon phase (at day thirteen of the lunar cycle according to the simulated lunar month in the laboratory) was selected for ptychography.…”

Section: Methodsmentioning

confidence: 99%

“…Trace metals have been shown to be indicators of certain steps of melanogenesis [4]. Our detailed study of the lunar cycle dependence of melanogenesis with single-pigment resolution based on transmission electron microscopy, X-ray fluorescence analysis (XRF) and light microscopy (LM) is published elsewhere [5,6]. In this study we added for the first time scanning coherent X-ray diffractive imaging (ptychography) to the suite of non-destructive microscopy techniques on a clunio sample.…”

Section: Introductionmentioning

confidence: 99%

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Correlative XRF, ptychography and light microscopy on shielding pigments of Clunio larval ocelli

Falkenberg¹,

Fleissner²,

Alraun³

et al. 2018

J. Inst.

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The nature of retinal shielding pigment in the larval eyes of the midge Clunio marinus was studied by high resolution scanning X-ray fluorescence (XRF) analysis correlated with light microscopy (LM) and scanning coherent X-ray diffractive imaging (ptychography). Clunio is known as a unique model system to decipher mechanisms underlying the complex moon-related rhythms. Shielding pigment granules within the photoreceptor cells of their primitive eyes (ocelli) have been shown to change appearance in the same rhythm. We compared the relative amount of trace metals (Ca, Ni, Zn, Cu), obtained by XRF, within single melanosomes (diameter about 500 nm) and the surrounding photoreceptor cells with their optical density in the light microscope and -for the first time -the electron density derived from ptychography images. Though individual pigment granules can be identified and correlated in images of all three techniques the optical density, electron density and especially element concentrations within individual granules seem to vary independently. We discuss that the state of melanogenesis is not synchronized inside the ocellus. K: Analysis and statistical methods; Multi-modality systems; X-ray fluorescence (XRF) systems 1Corresponding author.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Correlative XRF, ptychography and light microscopy on shielding pigments of Clunio larval ocelli

Falkenberg¹,

Fleissner²,

Alraun³

et al. 2018

J. Inst.

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“…With a new optical setup at beamline P06 combining the micro-(optics and sample) and nanohutch (diffraction detector), we recently investigated a thin section of a Clunio-larva eye (see G. Falkenberg, et al [4] and references therein) by scanning X-ray microscopy employing X-ray fluorescence (XRF) and coherent diffraction contrast (ptychography). In this case, a stack of 50 Be CRLs with radius of curvature of R = 50 µm and geometric aperture of D = 300 µm was used to focus hard X rays (E = 12 keV) to slightly below 100 nm (FWHM) at a focal length of f = 22 cm.…”

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confidence: 99%

Scanning Hard X-Ray Microscopy Based on Be CRLs

et al. 2018

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“…The larvae are equipped with simple pigment-cup eyes (ocelli) in which the dense shielding pigment permits light access to the photosensitive rhabdomers only through a narrow pinhole resulting in a diffuse image of the environment. The pigment cup of these eyes becomes transparent during full moon [2]. Within the photoreceptor cells of the ocelli, melanin containing melanosomes form shielding pigment granules.…”

mentioning

confidence: 99%