Moorella sulfitireducens sp. nov., a thermophilic anaerobic bacterium isolated from a terrestrial thermal spring

Slobodkina, G. B.; Merkel, Alexander Y.; Kuchierskaya, Alexandra A.; Slobodkin, A. I.

doi:10.1007/s00792-022-01285-w

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…For example, M . perchloratireducens An10 expresses a cld-like activity [ 22 ] but does not harbor a canonical cld gene [ 25 ], and there is no evidence for such an activity or for a cld gene in M. glycerini DSM 11254 T [ 25 ]. Cld activity is a relevant factor because it could reduce the toxicity of the chlorite formed as an intermediate or end product of perchlorate reduction, thus enhancing growth.…”

Section: Resultsmentioning

confidence: 99%

“…The difference between the estimated and observed CO uptake (1761 µmol − 1155 µmol = 606 µmol) likely represents the CO incorporated into biomass and acetate, a known end product of homoacetogenic metabolism by M . glycerini DSM 11254 T [ 25 , 28 ]. Although acetate was not measured in this study, levels of approximately 8 mM can be inferred from the amount of CO not accounted for by chloride and biomass production.…”

Section: Resultsmentioning

confidence: 99%

“…Although numerous metabolically diverse Ni-COX have been described [ 21 ], only a few have been reported to reduce perchlorate (e.g., [ 22 , 23 , 24 , 25 ]), and none of these have been shown to couple perchlorate reduction with CO oxidation. The hyperthermophilic, sulfate-reducing crenarchaeote, Archaeoglobus fulgidus , grows as a carboxydotroph by coupling CO oxidation to sulfate reduction or acetogenesis [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Likewise, the thermophilic, homoacetogenic Firmicutes, Moorella glycerini and M . sulfitireducens , reduce perchlorate to chlorite and grow as carboxydotrophs using Ni-CODH [ 22 , 23 , 25 , 28 ], but their ability to couple perchlorate and CO has not been documented.…”

Section: Introductionmentioning

confidence: 99%

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Perchlorate-Coupled Carbon Monoxide (CO) Oxidation by Moorella glycerini, an Obligately Anaerobic, Thermophilic, Nickel-Dependent Carboxydotroph

Myers

King

2023

Microorganisms

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Many facultative and obligate anaerobes reduce perchlorate. Likewise, carbon monoxide (CO) oxidation has been documented in many aerobes, facultative anaerobes, and obligate anaerobes. A molybdenum-dependent CO dehydrogenase (Mo-CODH) and a nickel-dependent CO dehydrogenase (Ni-CODH) distinguish the former from the latter. Some Mo-dependent CO oxidizers (Mo-COX) couple CO oxidation to perchlorate reduction, but only at low concentrations of both under conditions that do not support growth in cultures. In contrast, CO-coupled perchlorate reduction has not been documented in Ni-dependent CO oxidizers (Ni-COX). To assess the potential for Ni-COX to reduce perchlorate, a model, obligately anaerobic homoacetogen, Moorella glycerini DSM 11254T, was cultivated with or without perchlorate, usiing CO or glycerol as its sole carbon and energy source. It grew with glycerol with or without perchlorate, and its maximum cell densities were only weakly affected by the perchlorate. However, when CO (at a 30% headspace concentration) was used as a carbon and energy source, perchlorate reduction supported greater cell densities and more rapid growth rates. The stoichiometry of CO uptake, perchlorate reduction, and chloride production were consistent with the cryptic pathway for perchlorate reduction with chlorite as an end product. Chloride production occurred abiologically in the medium due to a reaction between chlorite and the sulfide used as a reducing agent. These results provide the first demonstration of CO-coupled perchlorate reduction supporting growth in Ni-COX, and they provide constraints on the potential for perchlorate-coupled, anaerobic CO oxidation in engineered systems as well as terrestrial systems and hypothetical, sub-surface, serpentinite-hosted systems on Mars.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Perchlorate-Coupled Carbon Monoxide (CO) Oxidation by Moorella glycerini, an Obligately Anaerobic, Thermophilic, Nickel-Dependent Carboxydotroph

Myers

King

2023

Microorganisms

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Advanced aspects of acetogens

Poehlein,

Zeldes,

Flaiz

et al. 2024

Bioresource Technology

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Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts

Böer,

Engelhardt,

Lüschen

et al. 2024

FEMS Microbiology Ecology

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Thermophilic acetogenic bacteria have attracted attention as promising candidates for biotechnological applications such as syngas fermentation, microbial electrosynthesis and methanol conversion. Here, we aimed to isolate and characterize novel thermophilic acetogens from diverse environments. Enrichment of heterotrophic and autotrophic acetogens was monitored by 16S rRNA gene-based bacterial community analysis. Seven novel Moorella strains were isolated and characterized by genomic and physiological analyses. Two Moorella humiferrea isolates showed considerable differences during autotrophic growth. The M. humiferrea LNE isolate (DSM 117358) fermented carbon monoxide (CO) to acetate, while the M. humiferrea OCP isolate (DSM 117359) transformed CO to hydrogen and carbon dioxide (H2 + CO2) employing the water gas shift reaction. Another carboxydotrophic hydrogenogenic Moorella strain was isolated from the covering soil of an active charcoal burning pile and proposed as the type strain (ACPsT) of the novel species Moorella carbonis (DSM 116161T, CCOS 2103T). The remaining four novel strains were affiliated with Moorella thermoacetica and showed, together with the type strain DSM 2955T, the production of small amounts of ethanol from H2 + CO2 in addition to acetate. The physiological analyses of the novel Moorella strains revealed isolate-specific differences that considerably increase the knowledge base on thermophilic acetogens for future applications.

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Moorella sulfitireducens sp. nov., a thermophilic anaerobic bacterium isolated from a terrestrial thermal spring

Cited by 5 publications

References 43 publications

Perchlorate-Coupled Carbon Monoxide (CO) Oxidation by Moorella glycerini, an Obligately Anaerobic, Thermophilic, Nickel-Dependent Carboxydotroph

Perchlorate-Coupled Carbon Monoxide (CO) Oxidation by Moorella glycerini, an Obligately Anaerobic, Thermophilic, Nickel-Dependent Carboxydotroph

Advanced aspects of acetogens

Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts

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