Moraxella catarrhalis evades neutrophil oxidative stress responses providing a safer niche for nontypeable Haemophilus influenzae

Nicchi, Sonia; Giusti, Fabiola; Carello, Stefano; Lanfaloni, Sabrina Utrio; Tavarini, Silvia; Frigimelica, Elisabetta; Ferlenghi, Ilaria; Paccani, Silvia Rossi; Merola, Marcello; Delany, Isabel; Scarlato, Vincenzo; Maione, Domenico; Brettoni, Cecilia

doi:10.1016/j.isci.2022.103931

Cited by 8 publications

(4 citation statements)

References 74 publications

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“…To date, other defense strategies of M. catarrhalis , which allow the bacteria to overcome the inflammatory conditions, have also been documented. For example, M. catarrhalis evaded neutrophil oxidative stress responses via induction of less ROS and reduced NETosis in differentiated HL-60 neutrophils [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

The Direct Anti-Virulence but Not Bactericidal Activity of Human Neutrophil Elastase against Moraxella catarrhalis

Roszkowiak

McClean

Mirończuk

et al. 2023

IJMS

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Neutrophil elastase (NE) contributes to innate antibacterial defense at both the intracellular (phagocytosis) and extracellular (degranulation, NETosis) levels. Moraxella catarrhalis, a human respiratory pathogen, can exist in an inflammatory milieu which contains NE. No data are available on the action of NE against M. catarrhalis or on the counteraction of NE-dependent host defenses by this pathogen. Using time-kill assays we found that bacteria are able to survive and replicate in the presence of NE. Transmission electron microscopy and flow cytometry studies with NE-treated bacteria revealed that while NE admittedly destabilizes the outer membrane leaflet, it does not cause cytoplasmic membrane rupture, suggesting that the enzyme does not target components that are essential for cell integrity. Using LC-MS/MS spectroscopy we determined that NE cleaved at least three virulent surface proteins in outer membrane vesicles (OMVs) of M. catarrhalis, including OMP CD, McaP, and TbpA. The cleavage of OMP CD contributes to the significant decrease in resistance to serum complement in the complement-resistant strain Mc6. The cleavage of McaP did not cause any sensitization to erythromycin nor did NE disturb its drug action. Identifying NE as a novel but subtle anti-virulence agent together with its extracellularly not-efficient bactericidal activity against M. catarrhalis may facilitate the pathogen’s existence in the airways under inflammation.

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Section: Discussionmentioning

confidence: 99%

The Direct Anti-Virulence but Not Bactericidal Activity of Human Neutrophil Elastase against Moraxella catarrhalis

Roszkowiak

McClean

Mirończuk

et al. 2023

IJMS

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“…However, engagement of Siglec−14 by b−protein antagonizes the repressive effects of Siglec−5 by activating mitogen-activated protein kinase (MAPK) signaling (99). Moraxella catarrhalis limits ROS production by possibly binding to immunosuppressive Siglecs receptors (Siglec-5 and Siglec-9) and consequently suppressing NET generation (89). Furthermore, soluble Siglec-9 exhibited strong binding with a2-3-linked sialoglycoproteins adsorbed by P. aeruginosa.…”

Section: Suppression Of Net-mediated Receptors On Neutrophilmentioning

confidence: 99%

Neutrophil extracellular traps in bacterial infections and evasion strategies

Baz,

Hao,

Lan

et al. 2024

Front. Immunol.

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Neutrophils are innate immune cells that have a vital role in host defense systems. Neutrophil extracellular traps (NETs) are one of neutrophils’ defense mechanisms against pathogens. NETs comprise an ejected lattice of chromatin associated with histones, granular proteins, and cytosolic proteins. They are thought to be an efficient strategy to capture and/or kill bacteria and received intensive research interest in the recent years. However, soon after NETs were identified, it was observed that certain bacteria were able to evade NET entrapment through many different mechanisms. Here, we outline the recent progress of NETs in bacterial infections and the strategies employed by bacteria to evade or withstand NETs. Identifying the molecules and mechanisms that modulate NET release will improve our understanding of the functions of NETs in infections and provide new avenues for the prevention and treatment of bacterial diseases.

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“…As Ramsheh et al demonstrated, a lower relative abundance of Prevotella and a greater relative abundance of Moraxella were associated with the upregulation of proinflammatory responses and the downregulation of epithelial defense gene expression ( Ramsheh et al, 2021 ). Moraxella catarrhalis and non-typeable Haemophilus influenzae frequently exacerbate COPD via inflammatory oxidative stress ( Nicchi et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Sputum microbe community alterations induced by long-term inhaled corticosteroid use are associated with airway function in chronic obstructive pulmonary disease patients based on metagenomic next-generation sequencing (mNGS)

Yue,

Zhang,

et al. 2024

Front. Pharmacol.

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Objective: Inhaled corticosteroids (ICS) are widely used in chronic obstructive pulmonary disease (COPD) patients as a treatment option. However, ICS may also increase the risk of pneumonia and alter the composition of airway microbiota. In clinical application, the overuse of ICS exists pervasively and may potentially lead to adverse effects. Whether the long-term use of ICS confers enough benefit to COPD patients to justify its use so far remains unknown. Therefore, this study employed a single-center retrospective cohort study to compare alterations in airway function and the sputum microbial community structure between COPD patients who had undergone either long-term or short-term treatment with ICS.Methods: Sixty stable COPD patients who had used ICS were recruited and classified into the long-term use group (more than 3 months) and short-term use group (less than 3 months). The demographic features and clinical information of the subjects were investigated and their sputum samples were collected and subjected to metagenomic next-generation sequencing (mNGS).Results: The study found that compared with short-term ICS use, long-term ICS use did not further improve the clinical airway function, decrease the number of acute exacerbations, or decrease hospital readmission. In terms of sputum microbiota, the long-term use of ICS significantly altered the beta diversity of the microbial community structure (p < 0.05) and the top three phyla differed between the two groups. At the genus level, long-term ICS induced higher relative abundances of Abiotrophia, Schaalia, Granulicatella, Mogibacterium, Sphingobium, and Paraeggerthella compared to short-term ICS use. Additionally, alpha diversity was positively associated with clinical airway indicators (pre-bronchodilatory FEV1 and pre-bronchodilatory FVC) in the long-term ICS group. The relative abundances of Rothia, Granulicatella, Schaalia, and Mogibacterium genera had positive correlations with the eosinophil % (of all white blood cells). Conclusion: This study reveals the effect of long-term and short-term ICS use on sputum microbiota among COPD patients and provides a reference for the appropriate application of clinical ICS treatment in COPD patients.

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Moraxella catarrhalis evades neutrophil oxidative stress responses providing a safer niche for nontypeable Haemophilus influenzae

Cited by 8 publications

References 74 publications

The Direct Anti-Virulence but Not Bactericidal Activity of Human Neutrophil Elastase against Moraxella catarrhalis

The Direct Anti-Virulence but Not Bactericidal Activity of Human Neutrophil Elastase against Moraxella catarrhalis

Neutrophil extracellular traps in bacterial infections and evasion strategies

Sputum microbe community alterations induced by long-term inhaled corticosteroid use are associated with airway function in chronic obstructive pulmonary disease patients based on metagenomic next-generation sequencing (mNGS)

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