Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease

Abstract

A novel Gram-stain-negative, aerobic, coccus-shaped bacteria, designated ZY201115T, was isolated from the nasal cavity of a sheep with respiratory disease in Yunnan Province, south-west China, and its taxonomic affiliation was studied by applying a polyphasic approach. The strain grew at 18–41 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 8.0) and in 0.5–3.0% (w/v) NaCl (optimum, 1.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain is affiliated to the genus Moraxella with highest similarity to Moraxella bovis ATCC 10900T (96.6 %). Phylogenomic analysis based on 811 single-copy genes also indicated that the strain represents a novel species in the genus Moraxella and formed a deep and separated clade with Moraxella caviae NCTC 10293T. The highest genomic orthologous average nucleotide identity and digital DNA–DNA hybridization values between the strain and the type strains in the genus Moraxella were 73.7% ( M. caviae NCTC 10293T) and 25.3% ( Moraxella osloensis CCUG 350T), respectively. The G+C content of the complete genome sequence was 42.1 mol%. The predominant fatty acids (>5 %) were C18:1 ω9c, C17:1 ω8c, C12:03OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The major polar lipids were phosphatidylglycerol, cardiolipin, monolysocardiolipin, phosphatidylethanolamine and hemibismonoacylglycerophosphate. The major respiratory quinone was CoQ-8. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic characterizations, strain ZY201115T clearly represents a novel species of the genus Moraxella , for which the name Moraxella nasovis sp. nov. is proposed. The type strain is ZY201115T (=CCTCC AB 2021473T=CCUG 75922T).

Moraxella oculi sp. nov., isolated from a cow with infectious bovine keratoconjunctivitis

“…These biochemical results are consistent with other Moraxella species, such as M. bovis . Additional biochemical results obtained for Tifton1 T were compared with results for other type strains of Moraxella published previously [1, 15–17] (). Similar to the type species ( Moraxella lacunata CCUG 4441 T ), Tifton1 T is unable to assimilate any sugars; however, Tifton1 T can be differentiated from this strain by its catalase activity.…”

“…Based on genome annotation, putative genes coding for enzymes esterase (EC 3.1.2-), esterase lipase (EC 3.1.1.5), leucine arylamidase (EC 3.4.11.2), oxidase (EC 1.6.5.3) and catalase (EC 1.11.1.6) were identified in Tifton1 T . Biochemical testing was not performed to evaluate the function of the esterase, esterase lipase and leucine arylamidase genes, but all species of Moraxella detected from cattle described to date, as well as the type species of the genus, have been shown to have these functional enzymes [1,[15][16][17]. Thus, these particular biochemical tests are not useful to distinguish one species from another.…”