Moraxella osloensis Bacteremia in an Immunocompetent Child

Abstract

Moraxella osloensis has been reported in the literature as a rare cause of sepsis, central nervous system infection, chest infection, and endophthalmitis. In the present case, the organism was isolated from the blood of an 8-year-old immunocompetent boy. It could not be identified with VITEK-2 and API-20NE (bioMerieux SA, Marcy-l'Etoile, France). Latex-based bacterial antigen test (BD Directigen Meningitis Combo Test; Becton, Dickinson and Company, Sparks, Maryland, United States) was positive for Neisseria meningitidis A/Y. It was identified as M. osloensis using matrix-associated laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF) (Vitek MS, bioMerieux, Marcy-l'Etoile) (confidence level 99.9%) and 16S rRNA gene sequencing (99% sequence identity). The child responded well to ampicillin, and was discharged home after 4 days, on oral amoxicillin–clavulanic acid for the next 7 days. M. osloensis is a rare cause of bacteremia. It may masquerade as N. meningitidis in the routine tests performed in the routine microbiology laboratories. It is imperative to confirm the identification with MALDI-TOF or a molecular method to remove the false positive diagnosis of N. meningitidis, and to avoid the unnecessary use of prophylaxis with rifampicin or ciprofloxacin.

Composition of nasal bacterial community and its seasonal variation in health care workers stationed in a clinical research laboratory

“…From all the above it appears essential to expand our knowledge of human nasal bacterial communities and their diversity in health care settings. The 16S rRNA gene sequencing approach has been used successfully in the detection and identification of bacteria [ 24 – 26 ]. With the advent of next-generation sequencing, many non-cultivable bacteria in human-associated habitats have been reported [ 2 , 3 , 27 , 28 ].…”

Collection of Bacterial Community Associated with Size Fractionated Aerosols from Kuwait

“…All the DNA samples were also quantified through a Qubit fluorometer (Invitrogen, Carlsbad, CA, USA) The DNA concentration and yield is given in Table 2. The presence of bacterial DNA was checked through polymerase chain reaction employing the universal 16 s primers [2,[9][10][11]. High-quality and -purity DNA was used for library preparation using the NEBNext Ultra DNA library preparation kit (New England BioLabs, France).…”

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“…From all the above it appears essential to expand our knowledge of human nasal bacterial communities and their diversity in health care settings. The 16S rRNA gene sequencing approach has been used successfully in the detection and identification of bacteria [24][25][26]. With the advent of next-generation sequencing, many non-cultivable bacteria in human-associated habitats have been reported [2,3,27,28].…”