Morbus Haemolyticus Neonatorum Due to Rh Immunization

Nilsson, B.

doi:10.3109/00016346509156828

Cited by 2 publications

(1 citation statement)

References 101 publications

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“…No direct agglutination was detected. By rerosetting the cells twice within a few months, the titre of the specific antibody in the tissue culture fluid could be increased from 8 to 128, which corresponds to the average anti-D antibody titre in pregnancy sera [9] or in the serum of Rh-negative male responders (KS-P, KS-RP, KS-RRP in Table 1). On the average, 1.9 erythrocyte doses were injected before these responders reached at least the antibody titre of 64 (unpublished results from this laboratory).…”

Section: Resultsmentioning

confidence: 99%

Human Lymphoblastoid Cell Line Producing Specific Antibody against Rh‐Antigen D

Koskimies

1980

Scand J Immunol

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A lymphoblastoid cell line producing specific anti-Rh antibody against D determinant was established by Epstein-Barr virus (EBV) infection. The lymphocytes were from an immunized male donor with high titre of anti-D antibody. The cells were preselected by rosetting them with Rh-positive erythrocytes before EBV infection. The cell line produced specific antibody of IgG class, namely IgG1 subclass, but it also produced nonspecific IgM and IgG. By rerosetting the cell line, the specific antibody-producing population could be enriched and the antibody titre increased in the culture supernatant to the level of immune sera.

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Section: Resultsmentioning

confidence: 99%

Human Lymphoblastoid Cell Line Producing Specific Antibody against Rh‐Antigen D

Koskimies

1980

Scand J Immunol

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The Clinical Value of Amniotic Fluid Analysis in Pregnancies Complicated by RH‐Isoimmunization or Hepatosis

Jonasson¹

1973

Acta Obstet Gynecol Scand

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605 samples of amniotic fluid were obtained by abdominal amniocentesis in 363 late pregnancies. The liquor was analysed with respect to ΔE450 (the optical density at 450 nm), progesterone and total protein content. On 172 samples measurements of the acid and alkaline phosphatase activity were also performed. Most of the samples came from pregnancies complicated by isoimmunization, but the material also contained samples from women with hepatosis gravidarum or other complications as well as from normal pregnancies. The measurements have been studied in relation to duration of pregnancy, cord blood haemoglobin levels, cord serum bilirubin levels, infant birth weight, maternal serum bilirubin levels, maternal alkaline phosphatase levels, maternal age, order of pregnancy, maternal and infant blood groups. For part of the series the relationships between the parameters were studied by multiple regression analysis. The results indicate a rather weak correlation between infant cord blood haemoglobin levels and the other parameters. The ABO blood groups of mother and infant influence the levels of cord blood haemoglobin, cord serum bilirubin and amniotic fluid total protein. Amniotic fluid progesterone and ΔE450 values are correlated to amniotic fluid total protein levels. Low ΔE450 values indicate that the fetus is mature. Determinations of ΔLE460 before the 37th week show a better correlation with the condition of the infant than later ones. Order of pregnancy influences the total protein content of amniotic fluid in normal pregnancies. Determinations of acid and alkaline phosphatases in amniotic fluid do not give any clinically important information in cases of isoimmunization. Meconium contamination strongly increases alkaline phosphatase activity. Attempts to use the rapid method of Brown for oestrogen determinations in amniotic fluid proved to be difficult for technical reasons.

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Morbus Haemolyticus Neonatorum Due to Rh Immunization

Cited by 2 publications

References 101 publications

Human Lymphoblastoid Cell Line Producing Specific Antibody against Rh‐Antigen D

Human Lymphoblastoid Cell Line Producing Specific Antibody against Rh‐Antigen D

The Clinical Value of Amniotic Fluid Analysis in Pregnancies Complicated by RH‐Isoimmunization or Hepatosis

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