More than one way to toy with ChAT and VAChT

Castell, Xavier; Diebler, Marie‐Françoise; Tomasi, Monique; Bigari, Claire; Gois, Stéphanie De; Berrard, Sylvie; Mallet, Jacques; Israël, Maurice; Doležal, Vladimı́r

doi:10.1016/s0928-4257(01)00081-x

Cited by 25 publications

(22 citation statements)

References 45 publications

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“…Effects of DHA on cell growth and ChAT activity in serum-free medium Differentiation of NG108-15 cells induced by 100 nM dexamethasone and 0.2 mM dibutyryl-cAMP in presence of serum increased ChAT activity more than five times and attenuated cell growth estimated as a decrease of protein content by 34% (Table 1) in line with previous findings [12,13]. Deceleration of cell growth was connected with strong reduction of executioner caspase-3 activity.…”

Section: Resultssupporting

confidence: 90%

“…For all other experiments, 20,000 cells were seeded into each well of 24-well-plate and grown in 2 ml of basal or serum-free Dulbecco's modified Eagle's medium. Differentiation of cells was induced by 0.2 mM dibutyrylcAMP and 100 nM dexamethasone [12][13][14].…”

Section: Methodsmentioning

confidence: 99%

“…We have reported previously that differentiation of NG108-15 cells induced by cAMP and dexamethasone leads to morphological differentiation accompanied by a marked enhancement of the cholinergic gene locus transcription, the expression of ChAT activity, and depolarizationinduced calcium influx through neuronal voltageoperated calcium channels [12][13][14]. In the present experiments we utilized these cells to investigate a presumed effect of DHA on the expression of their cholinergic phenotype represented by ChAT activity.…”

Section: Introductionmentioning

confidence: 96%

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The Increase of Choline Acetyltransferase Activity by Docosahexaenoic Acid in NG108-15 Cells Grown in Serum-free Medium is Independent of its Effect on Cell Growth

et al. 2006

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We investigated the influence of the polyunsaturated docosahexaenoic acid (22:6n-3; DHA) on the constitutive expression of choline acetyltransferase (ChAT) in native and induced expression in differentiated cholinergic cells NG108-15 grown in serum-free medium. Elimination of serum-derived trophic support resulted in growth arrest and a strong decrease of ChAT activity. In either conditions, DHA largely rescued general indicators of cell growth and function, and partially prevented the decrease of ChAT activity. However, the maximal effect on general cell state in native and differentiated cells, and ChAT activity in native cells, was reached at or below 10 mumol/l of DHA. In contrast, maximal induction of ChAT activity in differentiated cells required about six times higher concentrations of DHA. These data thus demonstrate stimulatory effect of DHA on ChAT activity that is independent of its general cell protective properties.

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Section: Resultssupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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The Increase of Choline Acetyltransferase Activity by Docosahexaenoic Acid in NG108-15 Cells Grown in Serum-free Medium is Independent of its Effect on Cell Growth

et al. 2006

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“…The NG108-15 cells are a hybrid cell line constructed by somatic fusion of rat C6 glioma cells and mouse N18TG2 neuroblastoma cells. N18TG2 cells themselves do not express the differentiated phenotypes in the presence of RA (Bignami et al 1997), but NG108-15 and N18TG2 treated with a medium conditioned over C6 cells can differentiate by retinoic acid into functional cholinergic cells (Diebler et al 1998;Dolezal et al 2001;Castell et al 2002), indicating that the differentiation of N18TG2 neuroblastoma requires the cooperation of C6 glioma. The possibility remains that E 2 acted directly or indirectly on differentiation-inducing factor(s) derived from C6 glioma, and prompted further increase of ChAT gene expression.…”

Section: Discussionmentioning

confidence: 99%

Asymmetric regulation by estrogen at the cholinergic gene locus in differentiated NG108-15 neuronal cells

Yoshida

Aizawa

2010

Life Sciences

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“…For example, Schutz et al, 2001 suggested that separate transcriptional start sites within the cholinergic gene locus control VAChT and ChAT transcription, while additional elements are responsible for the specific transcriptional control of the entire locus in cholinergic versus noncholinergic neurons. Furthermore, earlier neurodevelopmental studies (Holler et al, 1996) and more recent culture studies (Castell et al, 2002) suggest the possibility that VAChT and ChAT are independently regulated either through separate mechanisms that control the activity of specific promoters, or through posttranscriptional mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Protracted effects of chronic oral haloperidol and risperidone on nerve growth factor, cholinergic neurons, and spatial reference learning in rats

et al. 2007

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The primary therapeutic agents used for schizophrenia, antipsychotic drugs, ameliorate psychotic symptoms; however, their chronic effects on cognition (or the physiologic processes of the brain that support cognition) are largely unknown. The purpose of this rodent study was to extend our previous work on this subject by investigating persistent effects (i.e., during a 14 day drug-free washout period) of chronic treatment (i.e., ranging from 45 days to six months) with a representative first and second generation antipsychotic. Drug effects on learning and memory and important neurobiological substrates of memory, the neurotrophin, nerve growth factor (NGF) and its receptors, and certain components of the basal forebrain cholinergic system were investigated. Behavioral effects of oral haloperidol (2.0 mg/kg/day), or risperidone (2.5 mg/kg/day) were assessed in an open field, a water maze task, and a radial arm maze procedure and neurochemical effects in brain tissue were subsequently measured by enzyme linked immunosorbant assays (ELISAs). The results indicated that both antipsychotics produced time-dependent and protracted deficits in the performance of a water maze procedure when compared to vehicle-treated controls, while neither drug was associated with significant alterations in radial arm maze performance. Interestingly, haloperidol, but not risperidone, was detectible in the rodent brain in appreciable levels for up to two weeks after drug discontinuation. Both antipsychotics were also associated with reduced levels of NGF protein in the basal forebrain and prefrontal cortex and significant (or nearly significant) decreases in phosphoTrkA protein and the vesicular acetylcholine transporter (depending on the brain region analyzed). Neither antipsychotic markedly affected TrkA or p75 NTR levels. These data indicate that chronic treatment with either haloperidol or risperidone may be associated with protracted negative effects

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More than one way to toy with ChAT and VAChT

Cited by 25 publications

References 45 publications

The Increase of Choline Acetyltransferase Activity by Docosahexaenoic Acid in NG108-15 Cells Grown in Serum-free Medium is Independent of its Effect on Cell Growth

The Increase of Choline Acetyltransferase Activity by Docosahexaenoic Acid in NG108-15 Cells Grown in Serum-free Medium is Independent of its Effect on Cell Growth

Asymmetric regulation by estrogen at the cholinergic gene locus in differentiated NG108-15 neuronal cells

Protracted effects of chronic oral haloperidol and risperidone on nerve growth factor, cholinergic neurons, and spatial reference learning in rats

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