Morfologia dos músculos sóleo e tibial anterior de ratos Wistar imobilizados e remobilizados em meio aquático

Kunz, Regina Inês; Coradini, Josinéia Gresele; Silva, Lígia Inez; Kakihata, Camila Mayumi Martin; Brancalhão, Rose Meire Costa; Bertolini, Gladson Ricardo Flôr; Ribeiro, Lucinéia de Fátima Chasko

doi:10.5585/conssaude.v13n4.5179

Cited by 3 publications

(8 citation statements)

References 24 publications

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“…Several studies with this and other muscles have data consistent with our findings. Kunz et al () using the same method of immobilization found similar results in the soleus and anterior tibial muscles regarding the reduction of area, but the smaller diameter was not altered in the anterior tibial. Pinheiro () in addition to finding similar data on atrophy in the gastrocnemius muscle, he described similar histology, with inflammatory cells, necrosis, nuclear internalization, and installation of fibrosis in the perimysium.…”

Section: Discussionmentioning

confidence: 74%

Morphological effects of whole‐body vibration on remobilization of the tibialis anterior muscle of Wistar rats

Zazula

Wutzke

Costa

et al. 2020

The Anatomical Record

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Therapeutic immobilization is a common treatment for the locomotor system; however, it causes loss of muscle due to disuse, leading to protein degradation and generating atrophy of muscle cells, ultimately changing functionality. In this sense, it is important for remobilization to be initiated early and performed with appropriate therapeutic strategies that enable tissue and functional recovery. One method of remobilization is physical exercise, among which whole body vibration (WBV) has been highlighted and mainly applied in people with reduced mobility. However, there are gaps on the morphological effects WBV has on muscle tissue, so in this study we analyzed the histomorphometry of the tibialis anterior muscle (TA) of Wistar rats remobilized using WBV. For the experiment, 32 male Wistar rats were used and divided into four groups (n = 8/group). Groups consisted of: control (CG), immobilized (IG), immobilized and remobilized freely (FG), and immobilized and remobilized with WBV (WG). After the experimental period, the TA was collected and processed for analysis in light microscopy. When compared to the control group, significant morphological changes were observed, which characterize muscle atrophy and reduction of all histomorphometric parameters of the TA of the immobilized animals. Remobilized animals showed improvement in all parameters, and the WBV was not different from the free remobilization, except for the reduction of central nuclei, which can be related to acceleration of the process of tissue regeneration. Thus, we can conclude that the WBV can have an impact on the acceleration of the muscle regenerative process, and may be beneficial in people with reduced mobility.

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Section: Discussionmentioning

confidence: 74%

Morphological effects of whole‐body vibration on remobilization of the tibialis anterior muscle of Wistar rats

Zazula

Wutzke

Costa

et al. 2020

The Anatomical Record

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“…A atrofia muscular causada pela imobilização é alvo de vários estudos, sendo mostrado, por meio de análise transversal de músculos de ratos submetidos à imobilização por diferentes períodos, que esta pode causar, além de alterações longitudinais [5], também na área de secção transversa do músculo [11,15,16], que está associada ao aumento da área de tecido conjuntivo presente em seu envoltório [6].…”

Section: Discussionunclassified

“…Mesmo conhecendo-se todos os efeitos lesivos da imobilização, ela é um tratamento frequentemente utilizado para lesões do sistema musculoesquelético [8,9], os processos de recuperação do músculo imobilizado e os métodos de remobilização não são, ainda, bem esclarecidos, sendo fonte para várias pesquisas. A forma mais comum de remobilização é por meio do exercício físico [10,11]. São estudados vários tipos de procedimentos visando identificar a contribuição mais efetiva na recuperação da musculatura debilitada devido à imobilização, utilizando exercícios de fortalecimento muscular e alongamentos [5], assim denota-se a importância em se estudar a contribuição destes exercícios para a recuperação do tecido muscular debilitado pela imobilização prolongada.…”

Section: Introductionunclassified

Análise longitudinal do músculo sóleo de ratos submetidos à remobilização por subida em escada

Baretta

Mattjie

Errero

et al. 2022

Rev Bras Fisiol Exerc

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Exercícios de fortalecimento muscular e alongamentos são amplamente utilizados para se descobrir um melhor protocolo pós-imobilização. O objetivo desta pesquisa foi verificar a interferência de protocolos de exercícios com subida de escada, no comprimento muscular e quantidade de sarcômeros em série ao longo do músculo sóleo de ratos submetidos à imobilização. Para tanto, foram utilizados 32 ratos machos, distribuídos em: GA: animais somente imobilizados e eutanasiados; GB: ratos imobilizados e com remobilização livre; GC: remobilizados com subida em escada sem peso, e realizadas 40 repetições e GD: remobilizados com subida, semelhante à GC, porém foram realizadas 60 repetições. Os animais foram imobilizados com o músculo sóleo esquerdo em encurtamento, e após imobilização, aos grupos GC e GD foi aplicado o protocolo acima descrito. Após 24 horas do término do experimento, os animais foram anestesiados e eutanasiados, e o músculo sóleo esquerdo dissecado e processado para estudo histológico longitudinal. As comparações foram realizadas com ANOVA unidirecional e pós-teste de Tukey, com nível de significância de 5%. A partir destas análises pode ser constatado que o protocolo de subida em escada com menos repetições foi o mais efetivo na remobilização.Palavras-chave: imobilização, exercício, sistema musculoesquelético.

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“…The Tibialis Anterior muscle was dissected and fixed in metacarn for 24 hours, stored in 70% alcohol until processing for inclusion in paraffin blocks, where they were first dehydrated in increasing alcoholic series and diaphanized in N-butyl alcohol. The blocks were cut transversely in a microtome to a thickness of five µm and stained in Hematoxylin and Eosin, and Masson's Trichromic (5,12).…”

Section: Collection and Preparation Of The Tibialis Anterior Musclementioning

confidence: 99%

“…As for the analysis of connective tissue, the GIMP 2.0 program was used, and the quantification and comparison of the total number of pixels in the image with the number of connective tissue pixels were performed, to define the total connective tissue present in the visual field. Thus, this quantification considered the percentage of pixels referring to the total connective tissue (CON), epimysium (EPI), perimysium (PER), and endomysium (END) (11,12).…”

Section: Histomorphological Analyzesmentioning

confidence: 99%

Whole-Body Vibration Promotes Beneficial Changes on the Anterior Tibial Muscle Histomorphometry of Hypothalamic Obese Rats

Zazula

Kirsch

Theodoro

et al. 2021

Muscle Ligaments and Tendons J

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Background. Due to the deleterious effects of obesity on muscle tissue and the search for tools to reverse these losses, it is important to understand the effect of physical exercises on the muscle structure of obese individuals. This study aimed to analyze the effect of wholebody vibration (WBV) on the histomorphological parameters of the anterior tibial muscle using the monosodium l-glutamate (MSG) obesity model. Methods. MSG-obese rats that were exposed to WBV on a vibrating platform with a frequency of 60 Hz, the amplitude of 2 mm, three times/week, 10 min/day, for eight weeks (from postnatal day (PN) 80 to PN136). The histomorphology of the anterior tibial muscle was evaluated. Results. When performing a WBV exercise, the animals showed altered structural responses in the MSG animals, such as reduced muscle mass, increased connective tissue, and nuclear activity. The WBV reduced the extracellular matrix and the nuclear activity in the MSG animals, showing efficiency in the protocol. Conclusions. Even with the aggressive character of the MSG model, the WBV exercise was able to induce repair to the muscle tissue of these animals, thus being a safe protocol for use in similar conditions.

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Morfologia dos músculos sóleo e tibial anterior de ratos Wistar imobilizados e remobilizados em meio aquático

Cited by 3 publications

References 24 publications

Morphological effects of whole‐body vibration on remobilization of the tibialis anterior muscle of Wistar rats

Morphological effects of whole‐body vibration on remobilization of the tibialis anterior muscle of Wistar rats

Análise longitudinal do músculo sóleo de ratos submetidos à remobilização por subida em escada

Whole-Body Vibration Promotes Beneficial Changes on the Anterior Tibial Muscle Histomorphometry of Hypothalamic Obese Rats

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