Morin a flavonoid exerts antioxidant potential in chronic hyperammonemic rats: a biochemical and histopathological study

Abstract

Plant flavonoids are emerging as potent therapeutic drugs effective against a wide range of free radical-mediated diseases. Morin (3,5,7,2',4'-pentahydroxyflavone), a member of flavonols, is an important bioactive compound by interacting with nucleic acids, enzymes and protein. In this study, we found that morin (30 mg/kg body weight) by oral administration offers protection against hyperammonemia by means of reducing blood ammonia, oxidative stress and enhancing antioxidant status in ammonium chloride-induced (100 mg/kg body weight; i.p) hyperammonemic rats. Enhanced blood ammonia, plasma urea, lipid peroxidation in circulation and tissues (liver and brain) of ammonium chloride-treated rats was accompanied by a significant decrease in the tissues levels of superoxide dismutase (SOD), catalase, reduced glutathione (GSH) and glutathione peroxidase (GPx). Morin administered rats showed a significant reduction in ammonia, urea, lipid peroxidation with a simultaneous elevation in antioxidant levels. Cotreatment with morin prevented the elevation of liver marker enzymes induced by ammonium chloride. The body weight of the animals decreased significantly on ammonium chloride administration when compared with control group. However, cotreatment with morin significantly prevented the decrease of the body weight caused by ammonium chloride. Hyperammonemic rats show liver fibrosis, steatosis, sinusoidal dilatation, etc., along with necrosis, microcystic degeneration in brain. All these changes were reduced in hyperammonemic rats treated with Morin, which too correlated with the biochemical observations. In conclusion, these findings indicate that morin exert antioxidant potential and offer protection against ammonium chloride-induced hyperammonemia. But the exact underlying mechanism needs to be elucidated.

Morin Mitigates Oxidative Stress, Apoptosis and Inflammation in Cerebral Ischemic Rats

“…The noxious effects of free radical on lipids prelude to the formation of lipid peroxidation end product, MDA, a toxic product which educe neuronal cell death and loss of brain function (Serteser et al, 2002). In our study, Morin treatment reduced the MDA level in MCAO rats, which is highly attributed to inhibitory potential of Morin on the oxidative insult (Subash and Subramanian, 2009). GSH, an effective non-protein thiol, act as potent quencher of ROS.…”

Morin, A Flavonoid, On Lipid Peroxidation And Antioxidant Status In Experimental Myocardial Ischemic Rats

“…Pretreatment with morin significantly increased the concentration of GSH in plasma and the heart and the activities of GPx and GST in the heart of ISO-induced rats. Previous report shows that the morin offers to protect against hyperammonemia by reduced oxidative stress and enhanced antioxidant activity in ammonium chloride-induced hyperammonemic rats (Subhash and Subramanian, 2009). …”

The flavonoid morin restores blood pressure and lipid metabolism in DOCA-salt hypertensive rats

“…10 Morin (3,5,7,2 ′ ,4 ′ -pentahydroxyflavone) is a flavonoid that belongs to the class of flavonols, which is widely distributed in plants and foods of plant origin; 9 it is especially abundant in guava leaves, 11 onion, and apple. 12 Morin has been reported to possess a variety of biological properties including antioxidant, 13 antiallergic, 14 anti-inflammatory, and anticarcinogenic effects. 15,16 In our previous study, we established the effects of morin on systolic and diastolic blood pressure, hepatic and renal function markers, nitric oxide metabolites, lipid peroxidation products, and antioxidant status in DOCA-salt hypertensive rats.…”