Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Boonanantanasarn, Kanitsak; Janebodin, Kajohnkiart; Suppakpatana, Prapan; Arayapisit, Tawepong; RODSUTTHI, Jit-aree; Chunhabundit, Panjit; Boonanuntanasarn, Surintorn; Sripairojthikoon, Wanida

doi:10.4012/dmj.2012-053-r

Cited by 12 publications

(9 citation statements)

References 38 publications

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“…Human periodontal ligament cells' osteogenic differentiation and matrix mineralization were studied by Boonanantanasarn et al [6] who used an aqueous extract of Morinda citrifolia leaves. Perhaps the phytochemical components of Noni leaves have a function in stimulating osteogenic differentiation and matrix mineralization.…”

Section: Discussionmentioning

confidence: 99%

“…The microtiter plates were agitated for 5 minutes at room temperature before being examined in a microplate reader set to 630 nm. The proportion of living cells was calculated using the given formula [6].…”

Section: Mtt (3-(45-dimethylthiazol-2-yl)-25 Diphenyltetrazolium Brom...mentioning

confidence: 99%

“…Phytochemical constituents of Noni leaves are rich in flavonoids, which are shown to promote collagen formation, osteogenic differentiation, and matrix mineralization. This may help as an osteoinductive agent for bone and periodontal tissue regeneration [6,7].…”

Section: Introductionmentioning

confidence: 99%

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An In Vitro Evaluation of Morinda citrifolia and Ocimum sanctum as Potential Storage Media to Maintain Cell Viability for Avulsed Teeth Using Collagenase Dispase Assay

Sagare¹,

Patil²,

Patıl³

et al. 2023

Cureus

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This study aimed to evaluate the protective effects of Ocimum sanctum extract and Morinda citrifolia juice on human periodontal ligament (PDL) cells after the reimplantation of avulsed teeth using a collagenasedispase test. Materials and methodsSixty-five human premolars, all of which would eventually need to be extracted, were split into three experimental groups: one treated with Hanks Balanced Salt Solution, another with Morinda citrifolia juice and Ocimum sanctum extract, and two control groups (positive and negative). There were 10 teeth in each control group and 15 teeth each were used in the experimental groups, with the first 30 minutes spent dry before being submerged in one of three experimental media for 45 minutes, followed by 30 minutes of treatment with collagenase and dispase II. The cells' vitality was measured by the trypsin dye exclusion technique. To determine how many PDL cells were still alive, An optical microscope and a hemocytometer were used. The data were analyzed using Kruskal-Wallis one-way ANOVA and Mann-Whitney U tests. ResultsThe percentage of viable PDL cells was greatest in Morinda citrifolia juice (85.18%), followed by HBSS (84.3%), and finally by Ocimum sanctum extract (68.04%). There was no significant difference in the number of viable PDL cells in Morinda citrifolia juice and HBSS. ConclusionThe results of this research suggest that Morinda citrifolia juice has potential as a storage medium and as an alternative to HBSS, within the study's constraints, considering its availability as well as economic feasibility.

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Section: Discussionmentioning

confidence: 99%

Section: Mtt (3-(45-dimethylthiazol-2-yl)-25 Diphenyltetrazolium Brom...mentioning

confidence: 99%

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An In Vitro Evaluation of Morinda citrifolia and Ocimum sanctum as Potential Storage Media to Maintain Cell Viability for Avulsed Teeth Using Collagenase Dispase Assay

Sagare¹,

Patil²,

Patıl³

et al. 2023

Cureus

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“…To induce osteogenesis in the SHED, SHED (3 × 10 3 /well) were cultured in the Human MSC Differentiation Medium Osteogenesis (CEFOgro™) in the absence or presence of TEES-10 ® (6:4) (100 μg/mL) for 24 h. After removing the medium, the cells were stained with 1% alizarin red S (pH 4.2; Sigma) at room temperature for 30 min to detect the calcification according to a previously published method [ 40 ], washed with distilled water and examined for calcification under a microscope. In addition, the color intensity was quantitated.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Anti-Inflammatory Effects of TEES-10®, a Mixture of Ethanol Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout, on Gingivitis and Periodontitis

Lee¹,

Kim

Hong

et al. 2022

Dentistry Journal

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Gingivitis and periodontitis are inflammatory disorders caused by dental plaque and calculus. These disorders often lead to tooth loss if not treated properly. Although antibiotics can be used, it is hard to treat them due to the difficulty in supplying effective doses of antibiotics to lesion areas and side effects associated with long-term use of antibiotics. In the present study, attempts were made to provide in vitro and in vivo evidence to support anti-inflammatory activities of TEES-10®, a mixture of ethanol extracts of Ligularia stenocephala (LSE) and Secale cereale L. sprout (SCSE) toward gingivitis and periodontitis by performing the following experiments. TEES-10® with a ratio of 6:4 (LSE:SCSE) showed the best effects in both stimulating the viability and inhibiting the cytotoxicity. In in vitro experiments, TEES-10® showed an ability to scavenge 2,2-diphenyl-1-picrylhydrazyl and superoxide radicals and remove ROS generated in periodontal ligament cells treated with lipopolysaccharide. TEES-10® also enhanced the viability of stem cells from human exfoliated deciduous teeth and stimulated the osteogenic differentiation of deciduous teeth cells. In in vivo experiments using rats with induced periodontitis, TEES-10® significantly decreased inflammatory cell infiltration and the numbers of osteoclasts, increased alveolar process volume and the numbers of osteoblasts, decreased serum levels of IL-1β and TNF-α (pro-inflammatory cytokines), and increased serum levels of IL-10 and IL-13 (anti-inflammatory cytokines). These results strongly support the theory that TEES-10® has the potential to be developed as a health functional food that can treat and prevent gingival and periodontal diseases and improve dental health.

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“…1 to remove any remaining debris, evaporated by a lyophilizer (Lyph Lock6 Model 77595-01; Labconco, Kansas City, MO, USA), and stored at −80°C. The four lyophilized extracts were dissolved in a culture medium and filtered through a 0.22- μ m cellulose acetate membrane filter (Pall Corporation, MI, USA) before cell treatment [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Assessment of the Antioxidant Activities among the Extracts of Different Parts of Clausena lansium (Lour.) Skeels in Human Gingival Fibroblast Cells

Zhu

Zuo

Jia

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Clausena lansium (Lour.) Skeels (wampee) is an outstanding natural plant with medicinal properties. The aim of this study was to compare the cytoprotective effects of four parts of wampee under oxidative stress. The aqueous extracts of leaf, peel, pulp, and seed were tested for the proliferation effects on human gingival fibroblast (HGF) cells and the protective effects in the hydrogen peroxide-induced HGF model. Furthermore, the total glutathione assay and identification of rutin by high-performance liquid chromatography were carried out to attempt to determine whether the cytoprotective effects were related to the total glutathione (GSH) stability and rutin content. The results showed that all of the extracts had no cytotoxicity to HGF at tested concentrations ranging from 50 to 5000 μg/ml during 24 h, and the leaf, pulp, and seed extracts increased proliferation of HGF at relatively high concentrations. All the extracts except for the seed extract significantly decreased the production of reactive oxygen species, and the peel extracts exhibited the most effective antioxidant effect. The leaf extract had the highest anticytotoxicity and GSH stabilization effect in the HGF challenged with hydrogen peroxide. In addition, the relative content of rutin in peel and leaf extracts was higher than that in pulp and seed. The results of GSH assay and rutin identification suggest that different cellular protective effects among the four parts of wampee are partially related to the GSH stabilization and rutin content. These findings provide a scientific basis for the antioxidant effect-related biological activities of wampee extracts.

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Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Cited by 12 publications

References 38 publications

An In Vitro Evaluation of Morinda citrifolia and Ocimum sanctum as Potential Storage Media to Maintain Cell Viability for Avulsed Teeth Using Collagenase Dispase Assay

An In Vitro Evaluation of Morinda citrifolia and Ocimum sanctum as Potential Storage Media to Maintain Cell Viability for Avulsed Teeth Using Collagenase Dispase Assay

In Vitro and In Vivo Anti-Inflammatory Effects of TEES-10®, a Mixture of Ethanol Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout, on Gingivitis and Periodontitis

Comparative Assessment of the Antioxidant Activities among the Extracts of Different Parts of Clausena lansium (Lour.) Skeels in Human Gingival Fibroblast Cells

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