Moringa oleifera leaves extract modulates toxicity, sperms alterations, oxidative stress, and testicular damage induced by tramadol in male rats

Abstract

Tramadol is a synthetic opioid analgesic used for moderate-to-severe pain structurally related to codeine and morphine, where their analgesic mechanism is a result of opioid and non-opioid mechanisms. This study was designed to evaluate the effects of Moringa oleifera leaves extract (MLE) on tramadol-induced testicular toxicity, sperm changes, testicular damage, and oxidative stress in male rats. Forty male albino rats were divided into four groups and treated for 4 weeks (group 1, as control; group 2, MLE; group 3, tramadol; group 4, MLE + tramadol). The relative body weight, relative testes weight, serum total testosterone, luteinizing hormone, follicle-stimulating hormone, sperm counts, vitality, total sperm motility, catalase, and superoxide dismutase activities were significantly decreased in tramadol-treated group when compared with the control group. In contrast, sperm abnormality, immotile sperm percent, testicular injury, and TBARS concentration in testes were significantly increased in the tramadol-treated group. In addition, histopathological examination for the tramadol-treated group has shown incomplete spermatogenesis, moderate degeneration in some seminiferous tubules with a significant decrease in the number of spermatogenic cells and depletion of Leydig cells. The administration of MLE with tramadol ameliorates the testicular toxicity, injury, sperm count, abnormalities, and oxidative stress induced by tramadol.

Moringa oleifera ethanolic extract ameliorates the testicular dysfunction resulted from HFD‐induced obesity rat model

“…Administration of MOLE in obesity-induced groups improved these parameters to similar levels as the control and MOLE groups. Our findings agree with previous studies, which reported that the administration of MOLE ameliorates tramadol-induced (Abd et al, 2020) or melamine-induced (Mansour et al, 2020) testicular injuries by abolishing oxidative stress and improving the seminal parameters in male rats.…”

The Mechanisms and Management of Age-Related Oxidative Stress in Male Hypogonadism Associated with Non-communicable Chronic Disease

“…Testosterone and oxidative stress reportedly improved in i n vitro models of Leydig cells treated with different antioxidant compounds [ 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 ]. Similar results were observed across various animal models of induced hypogonadism by using vitamins A and C [ 171 ], zinc [ 172 ], N -acetylcysteine (NAC) [ 173 , 174 ], gallic acid [ 175 ], lycopene [ 176 ], forskolin [ 177 ], resveratrol [ 167 , 178 ], Nigella sativa seed oil [ 179 ], Lycium chinense Mill [ 180 ], Eruca sativa seeds [ 181 ], Moringa oleifera leaves [ 182 ], Schisandra chinensis [ 183 ], Ojayeonjonghwan [ 184 , 185 ], and Qilin pills [ 186 ] ( Supplementary Table S2 ).…”

A Review on the Antidiabetic Properties of Moringa oleifera Extracts: Focusing on Oxidative Stress and Inflammation as Main Therapeutic Targets

“…Therefore, it is important for plants to be evaluated for their toxicity to know how safe they are for human use. Accumulative research shows that Moringa oleifera exhibits a lot of important biological properties such as antioxidant, anti-inflammation, anti-hyperglycemic properties over the past years proving that it is a good plant to use as an alternative therapeutic for diabetes ( Omodanisi et al, 2017b ; Gothai et al, 2017 ; Paula et al, 2017 ; Abd et al, 2020 ; Xiong et al, 2021 ). In vitro and in vivo studies that have been conducted to show that this plant has no lethal dose and is safe to use.…”