Moringa oleifera Pod Inhibits Inflammatory Mediator Production by Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cell Lines

Abstract

Pro-inflammatory mediators produced during inflammatory response have been demonstrated to initiate and aggravate pathological development of several chronic diseases. Plant bioactive constituents have been reported to exert anti-inflammatory activities. Various parts of Moringa oleifera have long been used as habitual diets and traditional remedy along the tropical region. Anti-inflammatory activity of boiled M. oleifera pod extract was assessed by measuring pro-inflammatory mediator expression in the lipopolysaccharide-induced murine RAW264.7 macrophage cells. Prior treatment with 31-250 μg/mL M. oleifera extract for 1 h inhibited elevation of mRNA and protein level of interleukine-6, tumor necrosis factor-alpha, inducible nitric oxide synthase, and cyclooxygenease-2, induced by lipopolysaccharide for 24 h in a dose-dependent manner. The suppressive effect was mediated partly by inhibiting phosphorylation of inhibitor kappa B protein and mitogen-activated protein kinases. These results indicate that the anti-inflammatory activity from bioactive compounds present in the M. oleifera pod constituents may contribute to ameliorate the pathogenesis of inflammatory-associated chronic diseases.

An Ethyl Acetate Fraction of Moringa oleifera Lam. Inhibits Human Macrophage Cytokine Production Induced by Cigarette Smoke

“…An inhibitor of NF-κB (IκB) protein, which is the target for anti-inflammatory drug development [37], are principally supposed to inhibit p65-containing complex of NF-κB with noticeably high affinity [38]. Some reports have suggested that MO inhibits kinases upstream of NF-κB, IKB kinase and the MAP kinases [39], which are induced by oxidative stress. This would be consistent with our finding that basal activity was not affected, only that induced by LPS and CSE.…”

Review on biological and immunomodulatory properties of Moringa oleifera in animal and human nutrition

“…Alcohol extract from M. oleifera seeds has also shown anti-inflammatory activity against ovalbumin-induced respiratory tract inflammation in guinea pigs (Mahajan and Mehta, 2007). Bioactive compounds in M. oleifera pods can contribute to anti-inflammatory activity to improve the pathogenesis of chronic inflammatory diseases (Muangnoi et al, 2012).…”

Biological, nutritional, and therapeutic significance of Moringa oleifera Lam

“…This is an established fact that leaf, fruit, seed, and root extracts have been used since long back in enhancement of inflammation related disorders, for example, asthma, allergic rhinitis, atopic dermatitis, and rheumatoid arthritis (Chumark et al, ; Hamza, ; Lee et al, ; Muangnoi, Chingsuwanrote, Praengamthanachoti, Svasti, & Tuntipopipat, ). Anti‐inflammatory activities are possibly attributed due to the presence of 4‐[(α‐Lrhamnosyloxy) benzyl] isothiocyanate, 4‐[(4′‐O‐acetyl‐α‐ l ‐rhamnosyloxy) benzyl]isothiocyanate (Stohs & Hartman, ), quercetin, quercetin‐3‐O‐glucoside, kaempferol glucosides, (Coppin et al, ), 4‐(2‐O‐acetyl‐ α‐ l ‐rhamnosyloxy)benzyl isothiocyanate, 4‐(3‐O‐acetyl‐α‐rhamnosyloxy)benzyl isothiocyanate (Cheenpracha et al, ), 3,5‐dihydroxy‐6‐methyl‐2,3‐dihydro‐4H‐pyran‐4‐1, 9‐octadecenamide (Inbathamizh & Padmini, ), crypto chlorogenic acid (Vongsak, Gritsanapan, Wongkrajang, & Jantan, ), aurantiamide acetate, 1,3‐dibenzyl urea (Maheshwari, Yadav, Malhotra, Dhawan, & Mohan, ; Pandey et al, ) and 4(α‐ l ‐rhamnosyloxy)‐benzyl glucosinolate (Galuppo et al, ).…”