Moringa oleifera seed lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of Bak and NF-κB gene expression

Asaduzzaman, A.K.M.; Hasan, Imtiaj; Chakrabortty, Aninda; Zaman, Sadnima; Islam, Shaikh Shohidul; Ahmed, Fazle Rabbi Shakil; Kabir, K.M. Ahsanul; Nurujjaman, Md.; Uddin, Md Belal; Alam, Mohammad Taufiq; Shaha, Ranajit Kumar; Kabir, Syed Rashel

doi:10.1016/j.ijbiomac.2017.10.070

Cited by 32 publications

(13 citation statements)

References 36 publications

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“…Also, extracts derived from seeds were studied for their anti-proliferative effect on breast cancer MCF7 [ 38 ]. Finally, a Moringa oleifera seed lectin was purified and its anti-proliferative activity was studied against Ehrlich ascites carcinoma (EAC) cells, demonstrating that the cell growth inhibition was due to the induction of apoptosis [ 39 ]. To the best of our knowledge, this is the first investigation on possible Moringa oleifera anti-proliferative effects on melanoma colo38 cells.…”

Section: Resultsmentioning

confidence: 99%

Moringa oleifera Leaf Extracts as Multifunctional Ingredients for “Natural and Organic” Sunscreens and Photoprotective Preparations

et al. 2018

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Moringa oleifera has gained increasing popularity as a food supplement but not in the pharmaceutical and cosmetic area. The aim of this study was the preparation, characterization, and evaluation of extracts from the leaves of Moringa oleifera as a herbal sun care phytocomplex. Three different extracts of Moringa oleifera leaves, from Senegal, have been prepared and chemically characterized in the phenolic fraction by HPLC-DAD and Folin–Ciocalteu test. To explore photoprotective properties, an extensive evaluation of UV filtering, antioxidant (DPPH, FRAP, ORAC, PCL), and anti-hyperproliferative (human melanoma Colo38 cells) capacities have been conducted. Furthermore, a formulation study regarding cosmetic prototypes has been carried out in order to determine the Sun Protection Factor (SPF), which was assessed in vitro. The extracts were demonstrated to confer significant values of protection, with an SPF 2, that corresponds to a 50% protection against UV-B rays, at concentrations as low as 2% to 4%. Finally, the evaluation on potential irritation of the finished formulations was conducted by Patch Test and no significant irritant potential was observed. These evidence enlarged the already significant number of activities and potential uses of this plant, which is well-known for its importance in the medicinal and nutritional fields.

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Section: Resultsmentioning

confidence: 99%

Moringa oleifera Leaf Extracts as Multifunctional Ingredients for “Natural and Organic” Sunscreens and Photoprotective Preparations

et al. 2018

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“…An aqueous extract of Viscum articulatum Burm. F. (VAQE) stopped the cell cycle in G2/M in human leukemia cells and a Moringa oleifera seed lectin (MOSL) inhibited cell growth by arresting cell cycle in G2/M in Ehrlich-Lettre ascites carcinoma (EAC) cells [30]. Specifically, in the metastatic SW-620 colon cancer cell line, arrest was found in the S phase and was time-dependent.…”

Section: Discussionmentioning

confidence: 99%

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

et al. 2020

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A Tepary bean lectin fraction (TBLF) has been studied because it exhibits differential cytotoxic and anticancer effects on colon cancer. The present work focuses on the evaluation of the apoptotic mechanism of action on colon cancer cells. Initially, lethal concentrations (LC50) were obtained for the three studied cell lines (HT-29, RKO and SW-480). HT-29 showed the highest LC50, 10 and 100 times higher than that of RKO and SW-480 cells, respectively. Apoptosis was evaluated by flow cytometry, where HT-29 cells showed the highest levels of early and total apoptosis, caspases activity was confirmed and necrosis was discarded. The effect on cell cycle arrest was shown in the G0/G1 phase. Specific apoptosis-related gene expression was determined, where an increase in p53 and a decrease in Bcl-2 were observed. Expression of p53 gene showed the maximum level at 8 h with an important decrease at 12 and 24 h, also the phosphorylated p53(ser46) increased at 8 h. Our results show that TBLF induces apoptosis in colon cancer cells by p-p53(ser46) involvement. Further studies will focus on studying the specific signal transduction pathway.

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“…Cell cycle evaluation was accomplished using flow cytometry as described by Asaduzzaman et al (2018). Treatment with 3.0 mg kg −1 day −1 TDSL for five consequent days, treated and untreated EAC cells were collected from mice as well and then, repeatedly washed three times with PBS kept in cold temperature.…”

Section: Cell Cycle Evaluationmentioning

confidence: 99%

Trichosanthes dioica seed lectin inhibits Ehrlich ascites carcinoma cells growth in vivo in mice by inducing G ₀ /G ₁ cell cycle arrest

et al. 2021

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Trichosanthes dioica seed lectin (TDSL), having a molecular mass of 57 ± 2 kDa was purified in an alternative way. For the purification process, the galactose-sepharose-4B affinity column was used. The purified TDSL agglutinated human and mouse erythrocytes at the minimum concentration of 8 μg/ml. d-lactose and d-galactose were the most potent inhibitory sugars as observed. The purified lectin was a glycoprotein having 3.0% of a neutral sugar. The lectin exhibited maximum activity up to 60°C and pH range from 7.0 to 10.0 and stable up to 4.0 M urea as tested.

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Moringa oleifera seed lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of Bak and NF-κB gene expression

Cited by 32 publications

References 36 publications

Moringa oleifera Leaf Extracts as Multifunctional Ingredients for “Natural and Organic” Sunscreens and Photoprotective Preparations

Moringa oleifera Leaf Extracts as Multifunctional Ingredients for “Natural and Organic” Sunscreens and Photoprotective Preparations

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

Trichosanthes dioica seed lectin inhibits Ehrlich ascites carcinoma cells growth in vivo in mice by inducing G ₀ /G ₁ cell cycle arrest

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Moringa oleifera seed lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of Bak and NF-κB gene expression

Cited by 32 publications

References 36 publications

Moringa oleifera Leaf Extracts as Multifunctional Ingredients for “Natural and Organic” Sunscreens and Photoprotective Preparations

Moringa oleifera Leaf Extracts as Multifunctional Ingredients for “Natural and Organic” Sunscreens and Photoprotective Preparations

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

Trichosanthes dioica seed lectin inhibits Ehrlich ascites carcinoma cells growth in vivo in mice by inducing G 0 /G 1 cell cycle arrest

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Trichosanthes dioica seed lectin inhibits Ehrlich ascites carcinoma cells growth in vivo in mice by inducing G ₀ /G ₁ cell cycle arrest