Morphactin Substantially Induced the Fourth Internode Growth in Decapitated Tulips: Relevance to Endogenous Levels of Indole-3-Acetic Acid

Ueda, Junichi; Góraj, Justyna; Wegrzynowicz-Lesiak, E.; Miyamoto, Kazuaki

doi:10.2478/v10290-012-0025-5

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Thus, a possible mode of action of morphactin could be the blocking of auxin efflux from cells, thereby resulting in an increase of free IAA inside the cells (Hasenstein & Kaldewey 1984). After application of morphactin above the third node of decapitated tulips, the stimulated growth of the fourth internode was clearly observed (Ueda et al 2012). Endogenous levels of IAA in the fourth internode after the treatment of auxin polar transport inhibitors were determined using gas-liquid chromatography-mass spectrometry (GC-MS) and gas-liquid chromatography-mass spectrometry selected ion monitoring system (GC-SIM) with deuterium labeled IAA (d5-IAA) as an internal standard.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we have found that the application of morphactin above the third node in decapitated tulips substantially induced the fourth internode growth (Ueda et al 2012). It was also established that the elongation growth of the excised fourth internode in tulip shoots substantially depended on the initial length of the fourth internode (Saniewski et al 2010).…”

Section: Introductionmentioning

confidence: 94%

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Effects of auxin polar transport inhibitors on the growth of the excised fourth internode in tulips

Wegrzynowicz-Lesiak

Góraj

Miyamoto

et al. 2013

Journal of Horticultural Research

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Effects of auxin polar transport inhibitors: methyl 2-chloro-9-hydroxyfluorene-9-carboxylate (morphactin IT 3456), 2,3,5-triiodobenzoic acid (TIBA) and N-1-naphthylphthalamic acid (NPA) on elongation growth of the excised fourth internode of tulips (Tulipa gesneriana L.) cv. Apeldoorn were studied. After removal of flower bud, the continuing elongation of the excised fourth internode kept in water in normal or inverted position was observed, the elongation in inverted position being significantly higher than that in the normal position. On the other hand, the application of these inhibitors (0.2%, w/w in lanolin) in the place of removed flower bud substantially enhanced the elongation, the stimulation being much higher in the normal position than in the inverted one. When the inhibitors were applied in the middle or 1 cm from the base of the internode, the growth-promoting effect of these inhibitors was observed both in the upper and lower part of the internode, being greater in the upper part of the internode, regardless of the position of explants. Simultaneous application of indole-3-acetic acid (IAA) (0.1%, w/w in lanolin) at the place of the removed flower bud in the normal position with morphactin, TIBA and NPA applied in the middle or 1 cm from the base of the excised internode greatly stimulated the elongation, whereas almost no growthpromoting effect of these inhibitors was observed, in comparison to IAA. On the contrary, when IAA was applied on the base of the excised internode in the inverted position, the growth was inhibited, compared to that with lanolin only. The inhibitory effect of auxin was eliminated by the simultaneous application of morphactin, TIBA and NPA placed in the middle of the excised internode. These results suggest that auxin levels in the excised internode regulated by auxin polar transport play a crucial role in the regulation of its elongation growth.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Effects of auxin polar transport inhibitors on the growth of the excised fourth internode in tulips

Wegrzynowicz-Lesiak

Góraj

Miyamoto

et al. 2013

Journal of Horticultural Research

Self Cite

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Auxin, One Major Plant Hormone, in Soil

Ueda

Saniewski

Miyamoto

2016

Bioactive Compounds in Agricultural Soils

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Morphactin stimulates stem elongation and thickening in decapitated shoots of Bryophyllum calycinum Salisb.

et al. 2013

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A b s t r a c tMorphactin, methyl 2-chloro-9-hydroxyfluorene-9-carboxylate (IT 3456), is a synthetic growth regulator with a unique action affecting various morphogenetic and physiological processes in plants, and has been characterized as a specific inhibitor of auxin polar transport. Morphactin applied at the last internode in decapitated shoots of Bryophyllum calycinum substantially stimulated elongation and thickening of the internode. Benzyladenine applied alone little affected stem elongation and thickening. Simultaneous application of benzyladenine and morphactin showed a synergistic effect on thickening, while it did not on elongation. These results suggest that morphactin translocated basipetally from the top of the treated internode inhibits auxin polar transport from the internode, resulting in the accumulation of endogenous auxin for elongation and thickening in the treated internode of decapitated shoots of Bryophyllum calycinum.

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Morphactin Substantially Induced the Fourth Internode Growth in Decapitated Tulips: Relevance to Endogenous Levels of Indole-3-Acetic Acid

Cited by 3 publications

References 16 publications

Effects of auxin polar transport inhibitors on the growth of the excised fourth internode in tulips

Effects of auxin polar transport inhibitors on the growth of the excised fourth internode in tulips

Auxin, One Major Plant Hormone, in Soil

Morphactin stimulates stem elongation and thickening in decapitated shoots of Bryophyllum calycinum Salisb.

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