Morphine has an antinociceptive effect through activation of the okadaic-acid-sensitive Ser/Thr protein phosphatases PP2A and PP5 estimated by tail-pinch test in mice

Maeda, Takehiko; Hamabe, Wakako; Gao, Yuan; Fukazawa, Yohji; Kumamoto, Kazumasa; Kishioka, Shiroh

doi:10.1016/j.brainres.2005.07.033

Cited by 15 publications

(18 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result does not seem to give an explanation for our previous study, 5) if translocation of PP2A to the plasma membrane is essential for the antinociceptive effect of morphine. There are some reports suggesting that the physiological stimuli are sufficient to induce PP2A translocation to the plasma membrane, which triggers physiological response in vivo.…”

Section: Discussioncontrasting

confidence: 94%

“…Our previous study revealed that inhibition of PP2A and PP5 in the spinal and supraspinal tissue suppressed the antinociceptive effect of morphine in mice. 5) That result indicates that the OA-sensitive PP plays a functional role in the expression of morphine analgesia, whereas that does not provide any information on intracellular molecular mechanisms as a novel effector for opioid receptors. In the present study, we assessed activation of OA-sensitive PPs by measuring its distribution and activity in the subcellular fraction, which is referred to as translocation, as recently reported as evidence of OA-sensitive PP activation.…”

Section: Discussionmentioning

confidence: 95%

“…Our previous study revealed, in fact, that suppression of PP2A and PP5 expression by antisense-oligodeoxynucleotide against respective PP resulted in the significant, but partial, attenuation of antinociceptive effect of morphine in mice, while intracerebroventricular injection of OA produced greater inhibition of its effect. 5,11) That fact may offer the involvement of another OA-sensitive PP in morphine antinociception. PP4 might be a candidate for another OA-sensitive PP, 12) although it is unclear what role PP4 plays in antinociceptive effect of morphine.…”

Section: Discussionmentioning

confidence: 99%

“…5) In brief, a flattened clip (approximately 6-mm wide) was placed at the base of the tail. The pressure produced by the clip on the tail was adjusted to approximately 500 g. The nociceptive response was indicated by the time (latency) required for the mouse to respond to this pressure by vocalizing or biting at the clip.…”

Section: Subjectsmentioning

confidence: 99%

See 3 more Smart Citations

Increment of Activated Serine/Threonine Protein Phosphatase in Brain Membrane Fraction Synchronized with Antinociceptive Effect of Morphine in Mice

Maeda

Kiguchi

Kobayashi

et al. 2010

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

We have examined the involvement of serine/threonine protein phosphatase (PP) sensitive to okadaic acid (OA) in the antinociceptive effect of morphine in mice. The present study was performed to elucidate subcellular distribution and activity of OA-sensitive PPs in the brain when mice exposed to morphine. Subcutaneous administration of morphine (5 mg/kg) produced the antinociceptive effect with the maximum 30 min after its administration, evaluated by tail-pinch test. The antinociception was accompanied by an increment of activity in OA-sensitive PPs in the membrane fraction prepared from the whole brain of mice treated with morphine: The temporal profile of the morphine-induced increment of OA-sensitive PP activity was consistent with that of antinociceptive effects of morphine. The morphine-induced increase in OA-sensitive PP activity was dependent on the dose and attenuated by the concurrent administration of naloxone (1 mg/kg). To identify the subtype of OA-sensitive PPs in morphine-enhanced activity, we examined the level of PP2A and PP5, OA-sensitive PPs, in the subcellular fraction prepared from the whole brain of mice receiving morphine. Western blot revealed that morphine elicited the significant increase in the level of PP5, but not PP2A, in the membrane fraction, with the same peak time for the increment of PP5 as the antinociception. No significant change was observed in the level of OAsensitive PPs in the cytosolic fraction at any examined time after morphine. These results suggest that the translocation of PP5 to the membrane fraction is, at least in part, involved in the antinociceptive effect of morphine in mice.

show abstract

Section: Discussioncontrasting

confidence: 94%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Subjectsmentioning

confidence: 99%

See 2 more Smart Citations

Increment of Activated Serine/Threonine Protein Phosphatase in Brain Membrane Fraction Synchronized with Antinociceptive Effect of Morphine in Mice

Maeda

Kiguchi

Kobayashi

et al. 2010

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other animals received OA (1 pg/5 ml) or vehicle (saline, 5 mL) intracerebroventricularly (i.c.v.) under 1%-3% isoflurane (Escain; Pfizer, Tokyo, Japan) for general anesthesia and lidocaine (Xylocaine jelly 2%; AstraZeneca, Osaka, Japan) for local surface anesthesia, as described previously elsewhere (Maeda et al, 2005). Thirty minutes after the i.p.…”

Section: Methodsmentioning

confidence: 99%

Propofol Anesthesia Is Reduced in Phospholipase C–Related Inactive Protein Type-1 Knockout Mice

Nikaido

Furukawa

Shimoyama

et al. 2017

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The GABA type A receptor (GABA A -R) is a major target of intravenous anesthetics. Phospholipase C-related inactive protein type-1 (PRIP-1) is important in GABA A -R phosphorylation and membrane trafficking. In this study, we investigated the role of PRIP-1 in general anesthetic action. The anesthetic effects of propofol, etomidate, and pentobarbital were evaluated in wild-type and PRIP-1 knockout (PRIP-1 KO) mice by measuring the latency and duration of loss of righting reflex (LORR) and loss of tail-pinch withdrawal response (LTWR). The effect of pretreatment with okadaic acid (OA), a protein phosphatase 1/2A inhibitor, on propofol-and etomidate-induced LORR was also examined. PRIP-1 deficiency provided the reduction of LORR and LTWR induced by propofol but not by etomidate or pentobarbital, indicating that PRIP-1 could determine the potency of the anesthetic action of propofol. Pretreatment with OA recovered the anesthetic potency induced by propofol in PRIP-1 KO mice. OA injection enhanced phosphorylation of cortical the GABA A -R b3 subunit in PRIP-1 KO mice. These results suggest that PRIP-1-mediated GABA A -R b3 subunit phosphorylation might be involved in the general anesthetic action induced by propofol but not by etomidate or pentobarbital.

show abstract

Chemogenetic activation of central gastrin‐releasing peptide‐expressing neurons elicits itch‐related scratching behavior in male and female mice

Kiguchi

Fukazawa

Saika

et al. 2021

Pharmacology Res & Perspec

Self Cite

View full text Add to dashboard Cite

show abstract

Morphine has an antinociceptive effect through activation of the okadaic-acid-sensitive Ser/Thr protein phosphatases PP2A and PP5 estimated by tail-pinch test in mice

Cited by 15 publications

References 19 publications

Increment of Activated Serine/Threonine Protein Phosphatase in Brain Membrane Fraction Synchronized with Antinociceptive Effect of Morphine in Mice

Increment of Activated Serine/Threonine Protein Phosphatase in Brain Membrane Fraction Synchronized with Antinociceptive Effect of Morphine in Mice

Propofol Anesthesia Is Reduced in Phospholipase C–Related Inactive Protein Type-1 Knockout Mice

Chemogenetic activation of central gastrin‐releasing peptide‐expressing neurons elicits itch‐related scratching behavior in male and female mice

Contact Info

Product

Resources

About