Morpho-agronomic, biochemical and molecular analysis of genetic diversity in the Mesoamerican common bean panel

Abstract

The common bean (Phaseolus vulgaris L.) is of great importance to the food and nutritional security of many populations, and exploitation of the crop’s genetic diversity is essential for the success of breeding programs. Thus, the aim of the present study was to evaluate the genetic diversity of 215 common bean accessions, which included cultivars, obsolete cultivars, improved lines, and landraces using morpho-agronomic and biochemical traits, and amplified fragment length polymorphism markers (AFLP). Genetic parameters, box plots, Pearson’s correlation analysis, and Ward’s hierarchical clustering were used to analyze the data. The Jaccard similarity coefficient and neighbour-joining clustering method were used for molecular analysis. A wide variability among the accessions was observed for morpho-agronomic and biochemical traits. Selective accuracy (Ac) and broad-sense heritability (h2) values were high to intermediate for all traits, except seed yield. Ward’s hierarchical clustering analysis generated six groups. AFLP analysis also revealed significant differences among the accessions. There was no correlation between the differences based on genetic markers and those based on morpho-agronomic and biochemical data, which indicates that both datasets are important for elucidating the differences among accessions. The results of the present study indicate great genetic diversity among the evaluated accessions.

Morpho-Agronomic Genetic Diversity in Common Bean Landraces Based on Blup Values

“…The high heritability ( ) observed in the traits, expresses high genetic variability and correlation between phenotype and genotype. According to Resende and Duarte (2007) criteria, the selection accuracy ( ) was very high (≥ 0.90) for PH, DM, POP, SM and GY and high (≥ 0.70) for SPO and DF, 2020), Basavaraja et al (2021) and Nogueira et al (2021).…”

“…The genetic diversity for morpho-agronomic traits in common bean landraces has been studied by some authors (Stoilova et al, 2013;Nadeem et al, 2020;Nogueira et al, 2021) through several multivariate methods, such as discriminant analysis, principal components and cluster analysis (Diniz Filho, 2000;Cruz et al, 2020). There are other studies, based on morphological and molecular information, that combine these methods with the heat map (Fiore et al, 2020;Androcioli et al, 2020;Laskar et al, 2021;Mecha et al, 2022), however, the tool is still little explored in the study of genetic divergence for morphological traits, and very little for the study of pairwise distances.…”

Efficiency of RAPD and SCoT Markers in the Genetic Diversity Assessment of the Common Bean

“…The discovery of genetic relationships among plant genotypes based on DNA polymorphism may contribute to important knowledge leading not only to the simplification of the selection of germplasm for the breeding process but also to efficient conservation strategies [37,38] of genetic resources in gene banks. Many types of developed molecular markers have been used for the detection of genetic diversity in plants [30,31,[39][40][41][42][43][44][45].…”

Phenotypic and protein variations among selected cowpea (Vigna unguiculata L. Walp.) varieties

“…Cowpea cultivars have relatively limited genetic diversity owing to their inbreeding reproductive tendency. As a result, adding new accessions to crop breeding programmes is essential for increasing genetic diversity (Nogueira et al, 2021). Cowpea genetic progress can be hastened when extensive genetic variety and information on these genetic resources are available.…”