Morpho-anatomical structure and DNA barcode of Sonchus arvensis L.

Wahyuni, Dwi Kusuma; Rahayu, Shilfiana; Purnama, Putut Rakhmad; Saputro, Triono Bagus; Suharyanto, Suharyanto; Wijayanti, Nastiti; Purnobasuki, Hery

doi:10.13057/biodiv/d200841

Cited by 15 publications

(19 citation statements)

References 24 publications

(29 reference statements)

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“…The visualization results are not much different from the research conducted by Ilham et al (2022), in which rbcL gene in Achillea millefolium has a size of approximately 500 bp and matK has a size of 750 bp. Those studies used the same primer, which was also used by Wahyuni et al (2019).…”

Section: Dna Barcoding Pcr Amplification and Sequencingmentioning

confidence: 99%

“…The presence of bioactive components can be found by analyzing plant extracts, especially on the leaves (Phong et al 2022). Wahyuni et al (2019) add that secondary metabolites can be found in specific areas of plant organs. To comprehend the location of cells that store the secondary metabolites, anatomical research is necessary (Ilham et al 2022), since anatomical characteristics can also help identify plants.…”

Section: Introductionmentioning

confidence: 99%

“…Plant identification should be conducted using several characters, including morphological and molecular data to avoid ambiguous understanding, mainly when studying plant biological systems in pharmaceutical aspects (Wahyuni et al 2019;Ilham et al 2022). This study aimed to confirm and identify C. caudatus using morpho-anatomy and DNA barcoding.…”

Section: Introductionmentioning

confidence: 99%

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Morpho-anatomical characterization and DNA barcode of Cosmos caudatus Kunth.

Purnobasuki

RAKHASHIWI²,

JUNAIRIAH³

et al. 2022

Biodiversitas

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Abstract. Purnobasuki H, Rakhashiwi GA, Junairiah, Wahyuni DK, Putra RE, Raffiudin R, Soessilohadi RCH. 2022. Morpho-anatomical characterization and DNA barcode of Cosmos caudatus Kunth. Biodiversitas 23: 4097-4108. Secondary metabolites in plants have various benefits, therefore their distribution in plant body parts is important to study. The identification process of plants should be conducted using several characters, including morphological and molecular data. This study aimed to identify and confirm Cosmos caudatus Kunth using morpho-anatomy characters and DNA barcodes. The plants used were three samples taken from the Husada Graha Famili Park, Surabaya. The morpho-anatomical investigation was carried out descriptively, while the DNA barcoding study was processed by amplifying and sequencing the rbcL and matK genes. Morphological studies indicate that the roots and stems of C. caudatus are similar to the organs of dicots shrubs in general. The leaves have a pinnate type with two different colors on each side of the leaf. Compound flowers are cup-type with two types of flowers, namely disc flowers and ray flowers. Based on anatomical observation, all vegetative organs have the same tissue as dicotyledonous plants. The anatomical characteristic of C. caudatus lies in its trichomes due to its anthocyanin content and stomata which are anomocytic and anisocytic types. DNA barcoding results showed that the three sample plants had identical similarities with several Cosmos genera contained in the GenBank database with percentage identities values ??above 98%, query covers 96-100%, and e-values ??of 0.

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Section: Dna Barcoding Pcr Amplification and Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morpho-anatomical characterization and DNA barcode of Cosmos caudatus Kunth.

Purnobasuki

RAKHASHIWI²,

JUNAIRIAH³

et al. 2022

Biodiversitas

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“…The rbcL region has been used to differentiate four seagrass species from Thailand (Osathanunkul et al 2015), identify Halophila spp. from Sri Lanka (Liu et al 2020), and identify other flowering plants such as Sonchus arvensis (Wahyuni et al 2019) and genus Piper (Naim and Mahboob 2020).…”

Section: Introductionmentioning

confidence: 99%

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Stevanus

Pharmawati

2020

Biodiversitas

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Abstract. Stevanus, Pharmawati M. 2021. Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia. Biodiversitas 22: 50-57. West Lombok, Indonesia is one of the locations that is thought to have a quite high diversity of seagrass. Information on the diversity of seagrass species is important due to the important value of seagrass in the marine ecosystem. This research aimed to analyze biodiversity and phylogenetic of seagrass from Sekotong, West Lombok, Indonesia using DNA barcoding of rbcL gene. As many as 35 samples from seven morphologically identified species (Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halodule pinifolia, Halophila ovalis, and H. minor) were taken from four Gilis (small island) in Sekotong. The DNA was amplified for the rbcL gene and sequence analyses using BLAST were conducted to determine the species. Phylogenetic analyses were carried out using three evolutionary algorithms using Neighbor-Joining, Maximum Likelihood and Bayesian analysis with 1000 bootstrap. The rbcL gene was successfully amplified from all samples with a maximum length of 552 bp. The phylogenetic analysis showed that clades were split by family and genera where six clades were formed (Enhalus acoroides, T. hemprichii, Halophila complex, H. pinifolia, S. isoetifolium, and C. rotundata) with more than 95% of bootstrap values for Neighbor-Joining and Bayesian. The p-distance values between species were 0.008-0.097 and the polymorphic site was not found within species. The rbcL sequences only confirmed five seagrass species out of seven morphologically identified species and the sequences generated from this study cannot discriminate Halophila ovalis and H. minor.

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“…Sonchus arvensis L., an annual herb that belongs to Asteraceae, has an erect habit that reaches 64 cm in height [5]. In Indonesia, it is the 7 th most popular medicinal plant [6].…”

Section: Introductionmentioning

confidence: 99%

Growth, secondary metabolite production, and in vitro antiplasmodial activity of Sonchus arvensis L. callus under dolomite [CaMg(CO3)2] treatment

et al. 2021

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Malaria is still a global health problem. Plasmodium is a single-cell protozoan parasite that causes malaria and is transmitted to humans through the female Anopheles mosquito. The previous study showed that Sonchus arvensis L. callus has antiplasmodial activity. Several treatments are needed for callus quality improvement for antimalarial compound production. This study aimed to examine the effect of dolomite [CaMg(CO3)2] on growth (morpho-anatomical structure and biomass), secondary metabolite production, and in vitro antiplasmodial activity of S. arvensis L. callus. In this study, leaf explants were grown in Murashige and Skoog medium with a combination of 2,4-dichlorophenoxyacetic acid (2,4-D, one mg/L) and 6-benzyl amino purine (BAP, 0.5 mg/L) with dolomite (50, 75, 100, 150, and 200 mg/L). The 21 days callus ethanolic and methanolic extract were analyzed by gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). The antiplasmodial test was performed on a blood culture infected with Plasmodium falciparum strain 3D7 using the Rieckmann method. The results showed that dolomite significantly affected callus growth, metabolite profile, and in vitro antiplasmodial activity. Dolomite (150 mg/L) showed the highest biomass (0.590 ± 0.136 g fresh weight and 0.074 ± 0.008 g dry weight). GC-MS analysis detected four compounds from callus ethanolic extract. Pelargonic acid, decanoic acid, and hexadecanoic acid were major compounds. One new terpenoid compound is based on TLC analysis. S. arvensis L. callus has antiplasmodial activity with the IC50 value of 5.037 μg/mL. It was three times lower than leaf methanolic extract and five times lower than leaf ethanolic extract.

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Morpho-anatomical structure and DNA barcode of Sonchus arvensis L.

Cited by 15 publications

References 24 publications

Morpho-anatomical characterization and DNA barcode of Cosmos caudatus Kunth.

Morpho-anatomical characterization and DNA barcode of Cosmos caudatus Kunth.

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Growth, secondary metabolite production, and in vitro antiplasmodial activity of Sonchus arvensis L. callus under dolomite [CaMg(CO3)2] treatment

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