Morpho‐Functional Features of the Gonads of<i>Danio rerio</i>: the Role of Brain‐Derived Neurotrophic Factor

Cacialli, Pietro; D’Angelo, Livia; Girolamo, Paolo de; Avallone, Luigi; Lucini, Carla; Pellegrini, Elisabeth; Castaldo, L.

doi:10.1002/ar.23702

Cited by 15 publications

(11 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BDNF mRNA-expressing cells consisted of neurons, and their number increased mostly immediately after injury. The receptor TrkB increased at 24-48 h after injury, and this increase was sustained during the following 14 days [116]. Data by RNA-seq analysis approach confirmed the expression of other neurotrophins and their receptors after TBI in adult [118] zebrafish.…”

Section: Neurotrophins and Their Receptors Expression After Tbi In Zementioning

confidence: 59%

“…Similarly, double staining with the cell proliferation marker PCNA failed to show any co-expression of BDNF and PCNA in the same cells [115]. In contrast, double staining with neuronal makers, notably Huc/d, MAP2, and acetylated-tubulin, clearly identified BDNF-expressing cells as neuronal cells in the brain of adult zebrafish [116]. Further studies reported the distribution of TrkB receptor in different regions of zebrafish brain [117].…”

Section: Neurotrophins and Their Receptors Expression After Tbi In Zementioning

confidence: 91%

See 1 more Smart Citation

Neurotrophins Time Point Intervention after Traumatic Brain Injury: From Zebrafish to Human

Cacialli

2021

IJMS

Self Cite

View full text Add to dashboard Cite

Traumatic brain injury (TBI) remains the leading cause of long-term disability, which annually involves millions of individuals. Several studies on mammals reported that neurotrophins could play a significant role in both protection and recovery of function following neurodegenerative diseases such as stroke and TBI. This protective role of neurotrophins after an event of TBI has also been reported in the zebrafish model. Nevertheless, reparative mechanisms in mammalian brain are limited, and newly formed neurons do not survive for a long time. In contrast, the brain of adult fish has high regenerative properties after brain injury. The evident differences in regenerative properties between mammalian and fish brain have been ascribed to remarkable different adult neurogenesis processes. However, it is not clear if the specific role and time point contribution of each neurotrophin and receptor after TBI is conserved during vertebrate evolution. Therefore, in this review, I reported the specific role and time point of intervention for each neurotrophic factor and receptor after an event of TBI in zebrafish and mammals.

show abstract

Section: Neurotrophins and Their Receptors Expression After Tbi In Zementioning

confidence: 59%

Section: Neurotrophins and Their Receptors Expression After Tbi In Zementioning

confidence: 91%

Neurotrophins Time Point Intervention after Traumatic Brain Injury: From Zebrafish to Human

Cacialli

2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Next, the paraffin sections were washed three times in distilled H 2 O (1 min). Based on the morphological features described by previous studies [ 47 , 48 ], to examine cell and tissue morphology of ovary and testis, it has been used hematoxylin and eosin (HE) staining.…”

Section: Methodsmentioning

confidence: 99%

Expression of Nerve Growth Factor and Its Receptor TrkA in the Reproductive System of Adult Zebrafish

Cacialli

2022

Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

Nerve growth factor (NGF), a member of the neurotrophin family, has emerged as an active mediator in different crucial events in the peripheral and central nervous system. At the same time, several studies showed that this neurotrophin can also play a role in non-neuronal tissues (e.g., among gonads). In spite of a large number of studies present in mammals, investigations devoted to NGF and its receptor TrkA in the reproductive system of other animal models, such as teleost fish, are scarce. To increase our knowledge of NGF and its receptor in a vertebrate gonads model, the present report describes the expression patterns of ngf and trka mRNA in the testis and ovary of adult zebrafish. By using chromogenic and fluorescence in situ hybridization, we demonstrate that in the testis of adult zebrafish, ngf and its receptor trka are mainly expressed in spermatogony B and spermatocytes. In the ovary of this fish, ngf and trka are expressed at different stages of oocyte development. Altogether, these results show that this neurotrophin and its receptor have an important role in the reproductive system that is conserved during vertebrate evolution.

show abstract

“…We detected the expression of several genes with b-actin as the house keeping gene. The primer sequences for each gene are listed in Table 1 [24,[36][37][38].…”

Section: Analysis Of Mrna Expressionmentioning

confidence: 99%

Protective effects of phenformin on zebrafish embryonic neurodevelopmental toxicity induced by X-ray radiation

Gan

Guo

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Radiotherapy (RT) is a common treatment for head and neck cancers, but central nervous system function can be impaired by clinical radiation doses. This experimental study evaluated the protective efficacy of the anti-hyperglycaemic/anti-neoplastic agent phenformin against radiation-induced developmental toxicity in zebrafish embryos. Zebrafish embryos pre-treated with 25 lM phenformin 1 h before x-ray irradiation were compared to irradiation-only embryos for mortality, hatching rate, morphology, spontaneous movement, heart beat, larval swimming, activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), malondialdehyde content (MDA, a by-product of membrane lipid oxidation), and acetylcholinesterase (AChE) activity. In addition, expression levels of multiple genes related to neural development and apoptosis (sod2, bdnf, ache, p53, bax, and bcl-2) were compared by RT-PCR and associated protein expression levels by western blotting. Pre-treatment with phenformin increased hatching rate, spontaneous movement, heart beat, and larval motor activity, decreased mortality and malformation rate, increased SOD, CAT, and AChE activities, and reduced MDA compared to irradiation-only embryos. The mRNA expression levels of anti-apoptotic sod2, bdnf, ache, and bcl-2 were enhanced while mRNA expression of p53 and pro-apoptotic bax were reduced in the phenformin pre-treatment group. Further, p53, Bax, and c-H2AX (a biomarker of DNA damage) were downregulated while Bcl-2 and BDNF were upregulated by phenformin pre-treatment. Taken together, this study supports the protective efficacy of phenformin against radiation toxicity in zebrafish embryos by suppressing oxidative stress and ensuing apoptosis.

show abstract

Morpho‐Functional Features of the Gonads ofDanio rerio: the Role of Brain‐Derived Neurotrophic Factor

Cited by 15 publications

References 65 publications

Neurotrophins Time Point Intervention after Traumatic Brain Injury: From Zebrafish to Human

Neurotrophins Time Point Intervention after Traumatic Brain Injury: From Zebrafish to Human

Expression of Nerve Growth Factor and Its Receptor TrkA in the Reproductive System of Adult Zebrafish

Protective effects of phenformin on zebrafish embryonic neurodevelopmental toxicity induced by X-ray radiation

Contact Info

Product

Resources

About