Morpho-Molecular Identification of Lasiodiplodia theobromae Causing Root Rot of Mulberry in India

Kanyakumari, R.; Somasekhara, Y. M.; Basavaraj, S.; Deeksha, M. G.

doi:10.1007/s40009-022-01161-3

Cited by 3 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…L. theobromae (H-Lt) is a non-host-specific opportunistic fungal pathogen belonging to Botryosphaeriales and is known to cause black root rot disease where infections start as black lesions in the middle of roots and subsequently expand to form cankers [ 27 , 28 , 29 ]. Apart from a strain isolated from the Purple Millettia tree (H-Lt), it was also isolated from the decayed wood tissues of the Khaya tree (GenBank No.…”

Section: Resultsmentioning

confidence: 99%

Locally Isolated Trichoderma harzianum Species Have Broad Spectrum Biocontrol Activities against the Wood Rot Fungal Species through Both Volatile Inhibition and Mycoparasitism

Chan

Tan

Lee

et al. 2023

JoF

View full text Add to dashboard Cite

Pathogenic root/wood rot fungal species infect multiple urban tree species in Singapore. There is a need for sustainable and environmentally friendly mitigation. We report the local Trichoderma strains as potential biocontrol agents (BCAs) for pathogenic wood rot fungal species such as Phellinus noxius, Rigidoporus microporus, and Fulvifomes siamensis. Isolated Trichoderma strains were DNA-barcoded for their molecular identities and assessed for their potential as a BCA by their rate of growth in culture and effectiveness in inhibiting the pathogenic fungi in in vitro dual culture assays. Trichoderma harzianum strain CE92 was the most effective in inhibiting the growth of the pathogenic fungi tested. Preliminary results suggested both volatile organic compound (VOC) production and direct hyphal contact contributed to inhibition. SPME GC-MS identified known fungal inhibitory volatiles. Trichoderma harzianum strain CE92 hyphae were found to coil around Phellinus noxius and Lasiodiplodia theobromae upon contact in vitro and were possibly a part of the mycoparasitism. In summary, the work provides insight into Trichoderma inhibition of pathogenic fungi and identifies local strains with good potential for broad-spectrum BCAs against root/wood rot fungi in Singapore.

show abstract

Section: Resultsmentioning

confidence: 99%

Locally Isolated Trichoderma harzianum Species Have Broad Spectrum Biocontrol Activities against the Wood Rot Fungal Species through Both Volatile Inhibition and Mycoparasitism

Chan

Tan

Lee

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

“…Griffon & Maublanc, are of postharvest significance [3]. According to estimates, between 25 and 45 per cent of rotten, broken-textured, and microbiologically contaminated mangoes are wasted after harvest [4,5,6].…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Characterization of Major Postharvest Diseases of Mango

Anusha,

Indra,

Kannan

et al. 2023

IJPSS

View full text Add to dashboard Cite

Mango (Mangifera indica), which is rich in nutritive value is affected by many biological factors including diseases. Postharvest diseases viz., anthracnose caused by Colletotrichum gloeosporioides and stem end rot caused by Lasiodiplodia theobromae are of economic importance due to loss in quality and quantity after harvesting. The pathogens C. gloeosporioides and L. theobromae were isolated from five mango varieties collected from fruit markets and farmers’ field of major mango growing districts of Tamil Nadu. The mycelial, colony and conidial characters were identified morphologically. Molecular characterization was carried through polymerase chain reaction using universal primers ITS1 and ITS4. The sequencing was done and the sequences were deposited in Genbank. From phylogenetic analysis Colletotrichum gloeosporioides showed maximum identity of 100% and Lasiodiplodia theobromae showed maximum identity of 88%. The present study confirmed that pathogen associated with anthracnose and stem end rot was Colletotrichum gloeosporioides and Lasiodiplodia theobromae respectively.

show abstract

Emergence of Lasiodiplodia theobromae induced leaf necrosis in tea (Camellia sinensis [L.] O. Kuntze) from India

Ghosh,

Das,

Sorongpong

et al. 2024

Arch Microbiol

View full text Add to dashboard Cite

Morpho-Molecular Identification of Lasiodiplodia theobromae Causing Root Rot of Mulberry in India

Cited by 3 publications

References 10 publications

Locally Isolated Trichoderma harzianum Species Have Broad Spectrum Biocontrol Activities against the Wood Rot Fungal Species through Both Volatile Inhibition and Mycoparasitism

Locally Isolated Trichoderma harzianum Species Have Broad Spectrum Biocontrol Activities against the Wood Rot Fungal Species through Both Volatile Inhibition and Mycoparasitism

Morphological and Molecular Characterization of Major Postharvest Diseases of Mango

Emergence of Lasiodiplodia theobromae induced leaf necrosis in tea (Camellia sinensis [L.] O. Kuntze) from India

Contact Info

Product

Resources

About