Morpho-Physiological Characterization and Grouping (SAHN) of Chickpea Genotypes for Salinity Tolerance.

Kumar, Neeraj; Bharadwaj, Chellapilla; Satyavathi, C. Tara; Pal, Madan; Kumar, Tapan; Singhal, Tripti; Sachdeva, Supriya; Jain, Parul; Patil, B. S.; Soren, Khela Ram

doi:10.5958/2229-4473.2017.00045.3

Cited by 12 publications

(15 citation statements)

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“…With the anticipated scarcity of water in the future, terminal drought will continue to limit the chickpea production, with an estimated~40-60% decrease in average productivity (Kumar et al 2017). Under such a scenario, it is pertinent to identify genotypes that have a lower DSI but at the same time a higher yield and high biomass.…”

Section: Bp Laddermentioning

confidence: 99%

Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance

et al. 2018

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Diversity as a function of drought tolerance may be identified by morphological characters, and molecular tools used to find the most divergent genotypes for breeding programs for drought tolerance in future. The narrow genetic base of chickpea can be circumvented by using diverse lines in breeding programs. Forty chickpea genotypes were studied for their morphological and molecular diversity with an objective of identifying the most diverse drought-tolerant lines. In total, 90 alleles were detected with 3.6 alleles per locus. Polymorphism information content (PIC) values ranged from 0.155 to 0.782 with an average value of 0.4374 per locus. The size of amplified products ranged from 160 bp to 390 bp. Primer TA136 with eight alleles showed the highest PIC value of 0.7825, indicating its ability to differentiate the genotypes at molecular level. DARwin neighbour-joining tree analysis based on dissimilarity estimates was done for the molecular data and sequential agglomerative hierarchical non-overlapping (SAHN) grouping for the morphological data. It could clearly discriminate the tolerance and the sensitivity of genotypes. Two-dimensional principal coordinates analysis (PCoA) plot indicated good diversity for drought tolerance. The genetic similarity coefficients ranged from 0.115 (genotypes BGD72 to ICCV 5308) to 0.828 (genotypes ICCV 10316 to ICCV 92337).

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Section: Bp Laddermentioning

confidence: 99%

Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance

et al. 2018

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“…Chickpea (Cicer arietinum L.), one of the earliest food legume crop with a diploid chromosome number of 16 is cultivated in the tropics all over the world [1] and belongs to the family Fabaceae [2]. India being the largest producer of chickpea produces 68% of the total world production and about 9.21Mha area is under chickpea cultivation producing 8.88Mt [3]. Chickpea, characterized by different desi and kabuli cultivars has a profound nutritional and economic value [4].…”

Section: Introductionmentioning

confidence: 99%

“…A considerable decrease in chickpea productivity has been observed in the last thirty years due to change from lower to higher temperature regions of cultivation in South-East Asia and East Africa [5]. Presently, the world average productivity is about 995 kg/ ha which is very low [3] and has stagnated in recent years due to vulnerability of chickpea crop to various abiotic (drought, terminal heat, high salt, cold stress), and biotic (Ascochyta blight, Fusarium wilt, Helicoverpa) stresses [6]. Average losses upto 60% have been reported due to abiotic stresses globally in chickpea [7].…”

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confidence: 99%

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

et al. 2020

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Chickpea has a profound nutritional and economic value in vegetarian society. Continuous decline in chickpea productivity is attributed to insufficient genetic variability and different environmental stresses. Chickpea like several other legumes is highly susceptible to terminal drought stress. Multiple genes control drought tolerance and ASR gene plays a key role in regulating different plant stresses. The present study describes the molecular characterization and functional role of Abscissic acid and stress ripening (ASR) gene from chickpea (Cicer arietinum) and the gene sequence identified was submitted to NCBI Genbank (MK937569). Molecular analysis using MUSCLE software proved that the ASR nucleotide sequences in different legumes show variations at various positions though ASR genes are conserved in chickpea with only few variations. Sequence similarity of ASR gene to chickpea putative ABA/WDS induced protein mRNA clearly indicated its potential involvement in drought tolerance. Physiological screening and qRT-PCR results demonstrated increased ASR gene expression under drought stress possibly enabled genotypes to perform better under stress. Conserved domain search, protein structure analysis, prediction and validation, network analysis using Phyre2, Swiss-PDB viewer, ProSA and STRING analysis established the role of hypothetical ASR protein NP_001351739.1 in mediating drought responses. NP_001351739.1 might have enhanced the ASR gene activity as a transcription factor regulating drought stress tolerance in chickpea. This study could be useful in identification of new ASR genes that play a major role in drought tolerance and also develop functional markers for chickpea improvement.

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“…It is estimated that fifty per cent yield losses are caused by drought and heat stress (Gaur et al, 2012a ). Discovering the genotypic variation between the chickpea genotypes for drought tolerance is most important for the execution of breeding programs for chickpea (Kumar et al, 2018 ). Chickpea ( Cicer arietinum L.; Fabaceae family) is a diploid plant, containing chromosome number (2n = 16), self-pollinated and cool-season pulse.…”

Section: Introductionmentioning

confidence: 99%

Identification of Allelic Variation in Drought Responsive Dehydrin Gene Based on Sequence Similarity in Chickpea (Cicer arietinum L.)

Kumar

Tiwari²,

Bharadwaj

et al. 2020

Front. Genet.

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Chickpea (Cicer arietinum L.) is an economically important food legume grown in arid and semi-arid regions of the world. Chickpea is cultivated mainly in the rainfed, residual moisture, and restricted irrigation condition. The crop is always prone to drought stress which is resulting in flower drop, unfilled pods, and is a major yield reducer in many parts of the world. The present study elucidates the association between candidate gene and morpho-physiological traits for the screening of drought tolerance in chickpea. Abiotic stress-responsive gene Dehydrin (DHN) was identified in some of the chickpea genotypes based on the sequence similarity approach to play a major role in drought tolerance. Analysis of variance revealed a significant effect of drought on relative water content, membrane stability index, plant height, and yield traits. The genotypes Pusa1103, Pusa362, and ICC4958 were found most promising genotypes for drought tolerance as they maintained the higher value of osmotic regulations and yield characters. The results were further supported by a sequence similarity approach for the dehydrin gene when analyzed for the presence of single nucleotide polymorphisms (SNPs) and indels. Homozygous indels and single nucleotide polymorphisms were found after the sequencing in some of the selected genotypes.

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Morpho-Physiological Characterization and Grouping (SAHN) of Chickpea Genotypes for Salinity Tolerance.

Cited by 12 publications

References 0 publications

Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance

Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

Identification of Allelic Variation in Drought Responsive Dehydrin Gene Based on Sequence Similarity in Chickpea (Cicer arietinum L.)

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