Morpho-physiological disorders in in vitro culture of plants

Hazarika, B. N.

doi:10.1016/j.scienta.2006.01.038

Cited by 392 publications

(291 citation statements)

References 99 publications

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“…Several changes in the leaf structure of plants kept in vitro have been reported, such as the increase in size in the density of the stomata and the reduction in the stomatal control and in the thickness of the mesophyll, with a high proportion of intercellular spaces (Hazarika, 2006). In this study, broad intercellular spaces were not observed ( Figure 3A-F, 4A-G), and in plantlets with the addition of potassium silicate there was an increment in the cuticle and in the cell wall, providing greater thickening when compared to the vitroplants of the control treatment.…”

Section: Resultsmentioning

confidence: 99%

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

et al. 2017

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The aim of this study was to compare vitroplants Catasetum x apolloi grown under natural light and artificial light and different concentrations of potassium silicate, providing data on the anatomical differentiation that aids the acclimatization process of this species. Plants from in vitro seeding were used; 5 protocorms of approximately 0.5 cm were inoculated into vials with a capacity of 500 mL containing 100 mL of alternative culture medium plus potassium silicate (0.0, 0.5; 1.0 mL L -1 ), pH adjusted to 5.5 ± 0.5 and gelated with 4GL -1 agar before the autoclaving process. Cultures were maintained under natural light (TNE) and artificial light (TAE) for 90 days, and micromorphometric analysis was performed for polar and equatorial diameter, density and stomatal index, blade thickness in the central rib, and secondary veins. Applications in K 2 SiO 4 alternative medium provided the following: elongation of the hypodermis, thicker mesophyll, and more prominent midrib; elipptical guard cells; formation of epistomatal chamber; and lower stomatal density and stomatal with lower equatorial and polar diameters. The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses.Keywords: leaf anatomy, light, orchids, plants vitro. Anatomia e análise micromorfométrica foliar de Catasetum x apolloi Benelli & Grade com adição de silicato de potássio em diferentes fontes de luzResumo O objetivo desse trabalho foi comparar vitroplantas de Catasetum x apolloi cultivadas sob luz natural e luz artificial e diferentes concentrações de silicato de potássio, fornecendo dados sobre diferenciação anatômica que auxiliem no processo de aclimatação dessa espécie. Utilizou-se plantas provenientes da semeadura in vitro, 5 protocormos de aproximadamente 0,5 cm foram inoculados em frascos com capacidade para 500 mL contendo 100 mL de meio de cultura alternativo, acrescido de silicato de potássio (0,0; 0,5; 1,0 mL L -1 ), pH ajustado para 5,5 ±0,5 e gelificado com 4gL -1 de ágar antes do processo de autoclavagem. As culturas foram mantidas sob luz natural (TAA) e luz artificial (TAN) por 90 dias, e feitas análises micromorfométricas (diâmetro polar e equatorial, densidade e índice estomático, espessura do limbo na nervura central e nervuras secundárias). As aplicações de K 2 SiO 4 em meio alternativo, propiciaram: alongamento da hipoderme; mesofilo mais espesso e nervura central mais proeminente; células guardas elípticas; formação de câmaras supraestomáticas; menor densidade estomática e estômatos com menores diâmetros equatorial e polar. As condições que podem favorecer a aclimatação são menores intensidades de luz e menores doses de silicato de potássio.Palavras-chave: anatomia foliar, luminosidade, orquídeas, vitroplantas.

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Section: Resultsmentioning

confidence: 99%

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

et al. 2017

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“…No entanto, no tratamento com a dose de 10 g L -1 , as plantas manifestaram o fenômeno da hiper-hidricidade, definida como uma desordem morfofisiológica de plantas crescidas in vitro, resultando em baixa habilidade para o crescimento normal, além de problemas relativos à aclimatização. Este fenômeno é uma resposta da planta a estresses gerados por um ambiente inadequado ou impróprio para o crescimento e desenvolvimento in vitro, caracterizado por redução no teor de clorofila e proteínas, incremento no conteúdo de água e alteração na composição iônica dos tecidos (Hazarika, 2006;Hossain et al, 2010).…”

Section: Resultsunclassified

Cultivo in vitro de plântulas de orquídea em meios com diferentes concentrações de fertilizante mineral

et al. 2012

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In vitro cultivation of orchid seedlings in media with differents concentrations of mineral fertilizerThere are a number of medium formulations for growth of orchid seedlings in vitro, with different compositions and concentrations of salts, leading to varying plant responses to them. The objective of this work was to evaluate in vitro growth of orchid (Cattleya walkeriana Gardner) seedlings subjected to different rates of a NPK fertilizer added to the growth medium. The fertilizer Peters ® was used in the formulation NPK 10-30-20+Mg+micronutrients in the rates of 0.0; 1.0; 2.0; 3.0; 4.0; 5.0; and 10.0 g L -1 medium, with coconut milk (150 mL L -1 ), sucrose (20 g L -1 ) and activated charcoal (2 g L -1 ) and pH adjusted to 5.6. Six-month-old seedlings, previously germinated in Knudson C medium were transferred to 320 mL glass jars containing 35 mL of medium, using 10 plants per jar. The maximum production of dry matter from the aerial part at six months of age was estimated at 0.255 g/jar, with the medium added of 5.22 g L -1 of fertilizer. Considering the possibility of high salt concentration in the medium for some orchid species, the fertilizer rate needed to obtain 90% of maximum production was estimate at 3.55 g L -1 . There was a drastic growth reduction in root growth when using high fertilizer concentrations, producing fewer roots and shorter.

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“…Apesar das mudas de abacaxizeiro micropropagadas serem de fácil enraizamento, é importante observar o desenvolvimento das raízes durante a aclimatização, visto que, segundo Hazarika (2006), raízes formadas in vitro podem se apresentar pouco ramificadas, quebradiças, e até com inadequada conexão vascular.…”

Section: Características Fitotécnicasunclassified

“…Um dos maiores obstáculos para a aplicação prática dessa técnica é a dificuldade de transferir com sucesso mudas da condição in vitro para ex vitro, devido à grande diferença entre as duas condições ambientes (Hazarika, 2006). Isso pode ser um fator limitante, devido à morte das plantas, ocasionada principalmente pela perda excessiva de água, e à mudança de metabolismo heterotrófico para autotrófico (Barboza et al, 2006).…”

Section: Introductionunclassified

Morfofisiologia e anatomia foliar de mudas micropropagadas e aclimatizadas de abacaxizeiro cv. Smooth Cayenne em diferentes substratos

et al. 2012

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Morphophysiology and leaf anatomy of plantlets of pineapple cv. Smoth Cayenne acclimatized in different substratesThis study aimed to determine the optimal substrate and ideal time for acclimatization in plants of the 'Smooth Cayenne' pineapple and compare anatomically plantlets cultivated in vitro with acclimatized plants. Shoots were inoculated on MS with BAP (6.66 µM). After three subcultures, the buds were transferred to MS medium for 30 days. The media were solidified with 0.7% agar, and the pH adjusted to 5.8 before autoclaving (120ºC for 20 minutes). Cultures were kept in a growth room (25±1ºC, irradiance of 35 ì mol m-2 s-1 and 16 hours photoperiod). The shoots obtained in vitro were transplanted into plastic pots in a greenhouse, containing the following substrates: T1-Plantmax® + húmus (P + H), T2-Plantmax ® + vermiculite (P + V) and T3-Plantmax ® (P). Anatomical cuts were made on leaves of plants maintained in vitro and plants with 20, 40 and 60 days of acclimatization on different substrates. The use of Plantmax® + húmus substrate provided greater development in the acclimatization of plants. From 40 days of acclimatization, the plants showed anatomical features that may facilitate the adaptation to field conditions.

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Morpho-physiological disorders in in vitro culture of plants

Cited by 392 publications

References 99 publications

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

Cultivo in vitro de plântulas de orquídea em meios com diferentes concentrações de fertilizante mineral

Morfofisiologia e anatomia foliar de mudas micropropagadas e aclimatizadas de abacaxizeiro cv. Smooth Cayenne em diferentes substratos

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