Morphoanatomical characterization of filamentous green algae of district Lahore based on classical and modern microscopic technique

Abstract

Introduction For sample collection, four sites were selected, sites where fresh water were present, that is, Ravi syphon, BRB Lahore, Shahdara, and Head Baloki. The latitude and altitude of the locations were recorded at the time of collection. Results A total of 21 species of algae belonging to eight genera and four families were examined in the current studies. The collected samples were mainly filamentous and they were primarily green in color as the photosynthetic pigment found to be dominated was Chlorophyll. It was observed that all the genera varied mainly on the basis of cell shape, size, number of pyramids and on the arrangement of cells. The site mainly selected was those where fresh water was mainly found and at the time of collection latitude and altitude was also recorded. Conclusions The classical microscopic technique (Light Microscopy) and Modern microscopic technique (Staining Electron Microscopy) of some samples were done that, played a lively part in the correct identification of species as a slight difference was found among species that were only evident when the Modern microscopic technique was done.

Morphological characterization, growth appraisal, and probing biofuels potential of newly isolated Scenedesmus sp. from desert Cholistan

“…For LM living cells were harvested, washed twice with sterile distilled water and were observed under Nikon Eclipse 80i microscope equipped with a Nikon Digital Sight DS‐U1 camera (Nikon, Japan) as described by Alam et al (). For SEM, a protocol of Jaffer, Ashraf, and Shaheen () was followed. The algae cells were sputter coated with gold–palladium and subsequently observed under SEM (JEOL JSM‐5910) (Figure ).…”