Morphofunctional Characteristics of the Immune System in CBA and C57Bl/6g Mice

Shkurupiy, V. A.; Tkachev, V. O.; Потапова, О. В.; Лузгина, Н. Г.; Bugrimova, J. S.; Obedinskaya, K. S.; Zaiceva, N. S.; Чечушков, А. В.

doi:10.1007/s10517-011-1234-y

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…The half-life of Nrf2 is 7.5 to 15 min, [33][34][35] and it is regulated by Keap1-dependent ubiquitination and degradation. 36 Nrf2 is sequestered in the cytosol by Keap1, which regulates Nrf2 ubiquitination via Cul3-Keap1 complex. Under oxidative stress, the Cul3-Keap1-E3 ligase is inhibited, allowing Nrf2 translocation to the nucleus, binding to antioxidant response elements and initiating transcription of antioxidant enzymes.…”

Section: Hypertensionmentioning

confidence: 99%

Downregulation of Nuclear Factor Erythroid 2–Related Factor and Associated Antioxidant Genes Contributes to Redox-Sensitive Vascular Dysfunction in Hypertension

et al. 2015

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Abstract-Oxidative stress is implicated in vascular dysfunction in hypertension. Although mechanisms regulating vascular pro-oxidants are emerging, there is a paucity of information on antioxidant systems, particularly nuclear factor erythroid 2-related factor (Nrf2), a master regulator of antioxidants enzymes. We evaluated the vascular regulatory role of Nrf2 in hypertension and examined molecular mechanisms, whereby Nrf2 influences redox signaling in small arteries and vascular smooth muscle cells from Wistar Kyoto (WKY) and stroke-prone spontaneously hypertensive rats (SHRSP). Cells were stimulated with angiotensin II in the absence/presence of Nrf2 activators (bardoxolone/L-sulforaphane). Increased vascular reactive oxygen species production (chemiluminescence and amplex red) was associated with reduced Nrf2 activity in arteries (18%)

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Section: Hypertensionmentioning

confidence: 99%

Downregulation of Nuclear Factor Erythroid 2–Related Factor and Associated Antioxidant Genes Contributes to Redox-Sensitive Vascular Dysfunction in Hypertension

et al. 2015

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“…The CBA and C57BL/6 mouse strains develop different response to infection and radiation [20,22]. Some authors suggested that the observed phenomena might be explained by the differences in T-and B-lymphocytes [23], and peculiarities of T-cell differentiation [27]. Our results indicate that CBA and C57BL/6 mice vary in hemoglobin content in RBC, in number and size of platelets and in number of neutrophils.…”

Section: Discussionmentioning

confidence: 49%

“…The available data indicate that these two strains can be considered as contrasting ones regarding function of their immune system [20,22,23]. And both strains are used for studying the effects of IVIG [24,25].…”

Section: Introductionmentioning

confidence: 99%

Intravenous Immunoglobulins (IVIG) Enhance In vitro Activation of Neutrophils in C57BL/6 but not in CBA Mice

LY¹

2014

Immunome Res

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Background: Intravenous immunoglobulins (IVIG) are used in the treatment of inflammatory, autoimmune conditions and cancer. However, the precise mechanism of their action and the mechanisms that underlie responsiveness and resistance of some patients to the IVIG therapy remain unknown. The anti-cancer effect of IVIG might be mediated by neutrophils, which regulate tumor development. We studied in vitro effects of IVIG on neutrophils from two immunologically contrasting mouse strains, CBA and C57BL/6. Results: We examined total and differential WBC count, platelets count and the activity of normal neutrophils in intact CBA and C57BL/6 mice by method of luminol-dependent chemiluminescence (CL) of whole blood, and found significant difference in neutrophils, lymphocytes and platelets counts. Upon in vitro activation with opsonized zymozan (OZ), the C57BL/6 blood neutrophils showed no CL response at the absence of extracellular calcium while in the presence of calcium the CL responses of CBA and C57b blood were very similar. In the presence of IVIG, the calcium-dependent raise in OZ-activated CL response was registered only in C57BL/6 blood samples. Conclusions: For the first time we demonstrate that IVIG cause the calcium-dependent neutrophils priming in vitro in whole blood of C57BL/6 but not in CBA mice.

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“…This indicates a predominance of cellular elements (monocytes, macrophages, Kupffer cells) in the mononuclear phagocyte system (bone marrow-peripheral blood-liver) of C57BL/6 mice. Subsequently, this reflects a genetically determined higher power (quantitatively) of the system of mononuclear phagocytes in C57BL/6 mice compared to CBA mice [13]. It is believed that classical type activation (M1) is more characteristic in macrophages of C57BL/6 mice in contrast to the alternative one in macrophages of BALB/c and CBA mice [14][15][16].…”

Section: Introductionmentioning

confidence: 96%

Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

Русанов

Кожин

Tikhonova

et al. 2021

IJMS

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In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

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Morphofunctional Characteristics of the Immune System in CBA and C57Bl/6g Mice

Cited by 7 publications

References 10 publications

Downregulation of Nuclear Factor Erythroid 2–Related Factor and Associated Antioxidant Genes Contributes to Redox-Sensitive Vascular Dysfunction in Hypertension

Downregulation of Nuclear Factor Erythroid 2–Related Factor and Associated Antioxidant Genes Contributes to Redox-Sensitive Vascular Dysfunction in Hypertension

Intravenous Immunoglobulins (IVIG) Enhance In vitro Activation of Neutrophils in C57BL/6 but not in CBA Mice

Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

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