Morphofunctional characterization and antibacterial activity of haemocytes fromOctopus vulgaris

Abstract

This study focused on the morphological and functional characterization of the haemocytes from Octopus vulgaris as the first agents responsible for innate immunity. Three major haemocytes types were identified by light microscopy based on nucleus/cytoplasm ratio and the presence or absence of cytoplasm granules: haemoblast-like cells, hyalinocytes and granulocytes. The presence of three haemocyte populations was also confirmed by flow cytometry. Cytochemical characterization suggests that they perform different activities during humoral responses. Using the plate radial diffusion method we demonstrated the bactericidal activity of haemocytes in the presence of different bacteria strains. To investigate the presence of soluble biotic compounds responsible for antibacterial activity, using a disc diffusion method and the minimum inhibitory concentration, we have also tested the methanolic acid extract from these cells. Our results pave the way for the development of potent antibacterial drugs that could lead to several applications.

The Development and Characterization of a Cell Culture System from Indian Mud Crabs Scylla serrata

“…In addition, hemocyte cells from O. vulgaris were developed in a novel growth medium in which researchers have studied its antimicrobial activity and have differentiated and characterized their hemocyte cells using staining and flow cytometry (Troncone et al. ).…”

“…; Troncone et al. ). In these systems, hemocytes were morphologically differentiated and antibacterial activity was estimated by using various gram positive and gram negative bacteria (Troncone et al.…”

“…In these systems, hemocytes were morphologically differentiated and antibacterial activity was estimated by using various gram positive and gram negative bacteria (Troncone et al. ). The use of in vitro cell culture systems is the first choice to screen for the hazardous effects of pollutants because of their low cost, time efficiency, and low risk of ethical issues (Bakand ).…”

Morphological Characterization of Hemocytes and Histological Structure of the Hemopoietic Tissue \"White Body\" of the Cuttlefish \"Sepia Officinalis\" (Cephalopod:sepiidae).

“…13. Troncone et al (2014) identified three hemocyte types in octopus; granulocytes, hyalinocytes and haemoblastlike cells. The granulocytes are able to extend pseudopodia.…”

Understanding the cephalopod immune system based on functional and molecular evidence

“…Haemocytes are crucial in the cephalopod immune response [51,52,53]. Although these cells are vital to maintain the wellbeing in all different cephalopod species, the studies conducted about haemocytes are limited, and most of the observations have been performed on octopus species [45,50,54,55,56,57,58] with few descriptions available on Euprymna scolopes [59] and Sepia officinalis [46,60,61]. In one of the first studies of characterization based on morphology and immune capability of cells, Stuart [49], identified phagocytic and non-phagocytic "leucoblastic cells" in common octopus.…”