Morphofunctional Peculiarities of Platelet Rich Plasma and Its Application in Ophthalmology

Федосеева, Е. В.; Chentsova, E. V.; Borovkova, N. V.; Alekseeva, I. B.; Romānova, Inna

doi:10.18008/1816-5095-2018-4-388-393

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Применение фетальных клеток эпителия и кератобластов [7], амниотической мембраны [8], а также обогащенной тромбоцитами плазмы (ОбТП) [9] в настоящее время представляется наиболее перспективным подходом к лечению пациентов с хроническими эрозиями роговицы.…”

Section: актуальностьunclassified

“…в 3-4 раза больше, чем в неразбавленной крови. Тромбоциты человека, в свою очередь, обладают выраженным регенераторным эффектом за счет альфа-гранул, которые являются естественным депо факторов роста: тромбоцитарного фактора роста (PDGF), трансформирующего фактора роста (TGF-β), инсулиноподобного фактора роста (IGF-I, IGF-II), фактора роста фибробластов (FGFb), эпидермального фактора роста (EGF) и фактора роста эндотелия сосудов (VEGF) [9], влияющих на восстановление поврежденного эпителия за счет усиления процессов пролиферации, миграции и дифференцировки эпителиальных клеток [9]. Вместе с тем ОбТП содержит не только факторы роста, но и адгезивные молекулы (фибрин, фибронектин и витронектин) и цитокины, стимулирующие репарационные и анаболические процессы в поврежденных тканях, а также оказывающие противовоспалительное действие [10].…”

Section: Introductionunclassified

“…Вторичные посредники факторов роста инициируют нормальную, а не патологическую экспрессию генов, которая лежит в основе развития опухолей. Факторы роста не являются мутагенами и не блокируют механизмы обратной связи процессов репарации и регенерации тканей [9,10].…”

Section: Introductionunclassified

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Effect of Platelet-Riched Plasma on Corneal Epithelium Regeneration during Its Chronic Erosion (Experimental Study)

et al. 2021

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Relevance. In a number of pathological conditions accompanied by defects of the basal membrane, such as chemical or thermal burn, mechanical trauma, Schegren’s syndrome, herpetic keratitis processes of corneal epithelization are slowed down, and sometimes epithelization does not occur at all. Slow epithelization or its complete absence creates conditions for infection, thinning, and sometimes perforation of the cornea. That is why the problem of chronic corneal erosions is very relevant. The most perspective method of treatment is the use of autologous platelet-riched plasma (PRP). Presence of platelet growth factors, adhesive molecules and cytokines in PRP allows to use it for acceleration of regeneration of corneal defects, and the presence of live platelets allows to refer this procedure to autologous cell transplantations.Objective: To evaluate the clinical and morphological features of corneal epithelium regeneration in response to the application of PRP in conditions of experimental chronic corneal erosion (ECCE).Materials and methods. Researches were carried out on 12 rabbits of Chinchilla breed (24 eyes). At first stage the model of ECCE was reproduced for all animals: local ultraviolet (UV) irradiation with the exposure time of 45 min. on the preliminary de-epithelized corneal surface. At the second stage all animals under study were divided into three groups (4 rabbits in each group). I-st main group (MG) was instillated with PRP at a rate of 1 drop / 1 min within 10 minutes (total of 10 drops) once, in the II MG treatment was carried out according to the above method, every day for 5 days. In the control group (CG) PRP treatment was not applied.Results. 45-min. UV irradiation causes persistent ECCE up to 30 days of the experiment and reverts only after surface vessels are ingrowed in the affected area. Single use of PRP has insufficient therapeutic effect. Instillation within 5 days accelerates the cornea reparative regeneration in ECCE conditions. The absence of epithelial defect, newly formed vessels and corneal clouding was registered on the 9th day. Histologically, on the 30th day a normal multilayer non-squamous epithelium was found, while the stroma had an organized structure with no signs of inflammation.Conclusions. Therapeutic effect of PRP in ECCE conditions is based on reforming of the normal «adhesion complex» between epithelium, olfactory membrane and multifactor stimulation of reparative regeneration due to growth factors located in it, adhesive molecules and cytokines, as a result of which the typical corneal epithelium is restored.

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Section: актуальностьunclassified

Section: Introductionunclassified

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Effect of Platelet-Riched Plasma on Corneal Epithelium Regeneration during Its Chronic Erosion (Experimental Study)

et al. 2021

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“…The source of PDGF in serum is platelet alpha granules, although macrophages and endothelial cells can also produce it. Receptors to PDGF are located in the vascular wall on fibroblasts and smooth muscle cells [14,17]. In macrophage monocytes and fibroblasts PDGF stimulates chemotaxis, proliferation and expression of many genes [7].…”

Section: Introductionmentioning

confidence: 99%

The Significance of Platelet-Derived Growth Factor Concentrations in Endotheliotropic Natural Foci Infections

Vasilkova¹,

Кантемирова²,

Жидовинов³

2022

ArveMED

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In recent years, the clinical symptomatology of Astrakhan spotted fever and coxiellosis has worsened. The pathogenesis of these two natural focal, endothelium-dependent infections is rather complicated, multifactorial and not properly studied. The current clinical symptomatology is characterized by severe intoxication syndrome, frequently signs of multiple organ failure, development of hemocoagulation and vascular complications [3, 4, 5]. To date, no early markers of endothelial vascular damage have been proposed for use in practical health care, which enable accurately assessing the severity degree and predicting the further course of the rickettsiae and coxiellosis. Platelet-derived growth factor (PDGF), which is widely used in the diagnosis and prognosis of cardiovascular diseases, various injuries and diabetes mellitus, may become such a marker.

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Modern approaches to the surgical treatment of large full-thickness macular holes

et al. 2022

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Vitreoretinal surgery is an actively developing area of modern ophthalmic surgery. Intravitreal interventions in the central retina with large full-thickness macular holes deserve special attention. This article provides an overview of the scientific literature published in journals recommended by the Higher Attestation Commission, also presented in the scientific databases Scopus, PubMed, dedicated to modern techniques to the surgical treatment of large full-thickness macular holes. The main methods for closing defects in the macular area today are the use of various modifications of the inverted internal limiting membrane flap technique and the application of autologous platelet rich plasma in a macular hole. These techniques provide high anatomical and functional outcomes. Modifications of the inverted internal limiting membrane flap technique demonstrated effectiveness in such complex clinical situations as recurrent macular holes, concomitant high myopia, retinal detachment. Over the past 10 years, data on the use of autologous platelet-rich plasma for this group of patients appeared in the scientific literature. More accurate surgical procedures are required for the use of this technique compared with the standard methods, but this technique is applicable in all patients, does not require additional manipulations (blood sampling and centrifugation), additional equipment. Vitreoretinal interventions with the use of platelet-rich plasma are characterized by relative simplicity and ease of carrying out surgical procedures. However, it is important to consider the possibility of pseudouveitis development, the need for additional equipment. Since both of these methods demonstrate good anatomical results, the problem of choosing a technique in a particular clinical case remains. It was clear that the method of surgical intervention should be chosen, taking into account possible disadvantages and limitations to the method, as well as the skills of an ophthalmic surgeon. The lack of a unified approach to macular hole surgery encourages researchers to improve surgical techniques, develop and implement new modifications of surgical approaches.

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Morphofunctional Peculiarities of Platelet Rich Plasma and Its Application in Ophthalmology

Cited by 9 publications

References 15 publications

Effect of Platelet-Riched Plasma on Corneal Epithelium Regeneration during Its Chronic Erosion (Experimental Study)

Effect of Platelet-Riched Plasma on Corneal Epithelium Regeneration during Its Chronic Erosion (Experimental Study)

The Significance of Platelet-Derived Growth Factor Concentrations in Endotheliotropic Natural Foci Infections

Modern approaches to the surgical treatment of large full-thickness macular holes

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