Morphofunctional principles determining the changes in the adaptation strategy of ginseng (Panax ginseng C.A. Meyer) during its domestication

Burundukova, O. L.; Иванов, Л. А.; Иванова, Л. А.; Kiselev, Konstantin V.; Makhan’kov, V. V.; Lauve, L. S.; Khrolenko, Yu. A.; Burkovskaya, E. V.; Velivetskaya, T. A.; Ignatyev, A. V.; Музарок, Т. И.; Zhuravlev, Yu. N.

doi:10.1134/s0012496612050079

Cited by 2 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We hypothesized that an increased number of nucleoli in the leaves of P. ginseng observed in the present study can be initiated by the changes in adaptive strategy during plantation (i.e., from the stress tolerant in wild plants to the competitive and ruderal strategies of survival in cultivated plants). These alterations were shown to be associated with an increase in the acceptor load on the leaves, which are known to be a donor organ in ginseng [59] . Interestingly, the morphology of ginseng roots is mainly determined by the habitat, and therefore, the roots of the wild and cultivated plants are well distinguished.…”

Section: Discussionmentioning

confidence: 97%

Variation in the number of nucleoli and incomplete homogenization of 18S ribosomal DNA sequences in leaf cells of the cultivated Oriental ginseng (Panax ginseng Meyer)

Челомина

Rozhkovan

Voronova

et al. 2016

Journal of Ginseng Research

View full text Add to dashboard Cite

BackgroundWild ginseng, Panax ginseng Meyer, is an endangered species of medicinal plants. In the present study, we analyzed variations within the ribosomal DNA (rDNA) cluster to gain insight into the genetic diversity of the Oriental ginseng, P. ginseng, at artificial plant cultivation.MethodsThe roots of wild P. ginseng plants were sampled from a nonprotected natural population of the Russian Far East. The slides were prepared from leaf tissues using the squash technique for cytogenetic analysis. The 18S rDNA sequences were cloned and sequenced. The distribution of nucleotide diversity, recombination events, and interspecific phylogenies for the total 18S rDNA sequence data set was also examined.ResultsIn mesophyll cells, mononucleolar nuclei were estimated to be dominant (75.7%), while the remaining nuclei contained two to four nucleoli. Among the analyzed 18S rDNA clones, 20% were identical to the 18S rDNA sequence of P. ginseng from Japan, and other clones differed in one to six substitutions. The nucleotide polymorphism was more expressed at the positions 440–640 bp, and distributed in variable regions, expansion segments, and conservative elements of core structure. The phylogenetic analysis confirmed conspecificity of ginseng plants cultivated in different regions, with two fixed mutations between P. ginseng and other species.ConclusionThis study identified the evidences of the intragenomic nucleotide polymorphism in the 18S rDNA sequences of P. ginseng. These data suggest that, in cultivated plants, the observed genome instability may influence the synthesis of biologically active compounds, which are widely used in traditional medicine.

show abstract

Section: Discussionmentioning

confidence: 97%