Morphogenesis and plant regeneration from cultured endosperm of Emblica officinalis Gaertn

Sehgal, C. B.; Khurana, Sunilla

doi:10.1007/bf00269373

Cited by 10 publications

(16 citation statements)

References 3 publications

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“…It is reported that during germination the embryo releases certain gibberellin like substances, which may promote the endosperm proliferation (Ogawa 1964;Ingale and Hageman 1965). However, the mature endosperms of Achras sapota (Bapat and Narayanaswamy 1977), Santalum album (Lakshmi Sita et al 1980), Emblica officinalis (Sehgal and Khurana 1985) and Juglans regia (Cheema and Mehra 1982;Tulecke et al 1988) could proliferate without the association of embryo or pre-soaking of them in GA 3 .…”

Section: The Embryo Factormentioning

confidence: 94%

“…However, on IAA supplemented medium the callus gave rise to shoots in 100% cultures (Nag and Johri 1971). Sehgal and Khurana (1985) 45.…”

Section: Ricinus Communismentioning

confidence: 97%

“…In Emblica officinale, tracheidal cells and cambium like cells organized into vascular strands or nodules in the differentiation medium while in callusing medium tracheidal cells remained scattered. Differentiation of vascular strand in the callus accompanied shoot bud formation (Sehgal and Khurana 1985).…”

Section: Histologymentioning

confidence: 99%

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Endosperm culture: a novel method for triploid plant production

Thomas

Chaturvedi

2008

Plant Cell Tiss Organ Cult

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Triploid nature of endosperm is the characteristic feature of angiosperms and is formed as a result of triple fusion. Present review discusses the morphogenic response and production of triploid plantlets by endosperm culture. Both mature and immature endosperm used for culture initiation responded differently in cultures. A key factor for the induction of cell divisions in mature endosperm cultures is the initial association of embryo but immature endosperms proliferate independent of embryo. In almost all the parasitic angiosperms, endosperm shows a tendency of direct differentiation of organs without prior callusing, whereas in autotrophic taxa the endosperm usually forms callus tissue followed by differentiation of shoot buds, roots or embryos. The endosperm tissue often shows a high degree of chromosomal variations and polyploidy. Mitotic irregularities, chromosome bridges and laggards are the other important characteristics of endosperm tissues. Triploids are usually seed sterile and is undesirable for plants where seeds are commercially useful. However, in cases where seedlessness is employed to improve the quality of fruits as in banana, apple, citrus, grapes, papaya etc. the induction of triploid plants would be of immense use. Triploid plants have more vigorous vegetative growth than their diploid counterparts. Hence, in plants where the vegetative parts are economically useful, triploids are of good use. This review focuses on the progress achieved so far in endosperm culture to obtain triploid plants.

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Section: The Embryo Factormentioning

confidence: 94%

“…However, on IAA supplemented medium the callus gave rise to shoots in 100% cultures (Nag and Johri 1971). Sehgal and Khurana (1985) 45.…”

Section: Ricinus Communismentioning

confidence: 97%

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Endosperm culture: a novel method for triploid plant production

Thomas

Chaturvedi

2008

Plant Cell Tiss Organ Cult

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“…The addition of an auxin induces callus in the endosperm of Euphorbia [27] and Nigella [28]. Annona [29], Emblica [30],Juglans [9] and Lycium [31] require both an auxin and cytokinin, and for Prunus, [33] …”

Section: Immature Endospermmentioning

confidence: 99%

Plant Tissue Culture Manual

1992

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“…In vitro morphogenesis has been accomplished in many forest tree species [1,4,13,14]. Various species of Populus have been studied extensively for plant regeneration from protoplasts [12], callus [10], axillary buds [11], stem internodes [5,6] and immature embryo explant [8].…”

Section: Introductionmentioning

confidence: 99%

In vitro organogenesis and somatic embryogenesis from punctured leaf of Populus nigra � P. maximowiczii

Park

Son

1988

Plant Cell Tiss Organ Cult

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Various explant sources of Populus nigra x P. maximowiczii were used to examine the effects of growth hormones on morphogenesis in vitro. Initial experiments indicated that punctured leaves were superior to non-punctured ones for both callus growth and formation of shoots and roots on MS medium containing various types and concentrations of growth hormones. After 6 weeks in culture, an average of 178 shoots, 129 roots and 3.1 g fresh weight of callus were directly produced from the abaxial side of each punctured leaf. The best combinations of growth hormones for shoot, root and callus proliferation were 0.88 ~tM BA plus 0.05/~M 2,4-D, 0.44#M BA plus 2.69/~MNAA and 0.44/~M BA plus 2.26pM 2,4-D, respectively. Embryoids were also formed on callus derived from punctured leaves. The number of embryoids varied from 0 to 30 per punctured leaf. Adventitious shoots also developed simultaneously with the embryos. Embryoids were removed with a scalpel at the early developmental stages and placed on MS medium lacking growth regulators. Regenerated plantlets were transferred to pots containing vermiculite for normal growth in the greenhouse.

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Morphogenesis and plant regeneration from cultured endosperm of Emblica officinalis Gaertn

Cited by 10 publications

References 3 publications

Endosperm culture: a novel method for triploid plant production

Endosperm culture: a novel method for triploid plant production

Plant Tissue Culture Manual

In vitro organogenesis and somatic embryogenesis from punctured leaf of Populus nigra � P. maximowiczii

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