Morphogenesis Characteristics of Experimental Intestinal Anastomoses Made by Microsurgical Technique

Abstract

This article presents the results of morphologic examination of intestinal anastomoses (IAs) formed by a precision technique in an experiment. Processes of regeneration in the zone of bowel anastomoses made with the author's precision one-row continuous suture (POCS) (Ukraine patent UA 32940), the author's precision one-row interrupted suture (POIS) (Ukraine patent UA 119073) and a double-row Albert-Schmieden suture (DASS) on rabbits of butterfly breed (n=45) are reviewed. Histological examination on Days 1, 3, 5, 14 and 30 revealed differences in reparative processes depending on the type of anastomoses. The morphological pattern in the early term (Day 3) of reparation is caused by the predominance of lysis of collagen fibers; nevertheless, in the early term the quantitative evaluation of healing stages was higher in the two groups of precision anastomoses than in the DASS group (2.00±0.00 points vs. 1.33±0.33 points). Formation of granulation tissue and collagen started on Days 5-7 in the two groups of precision anastomoses versus Days 7-14 in the DASS group, and in the two groups the process reached Stage 5 of reparation (3.00±0.00 points), while in DASS group it reached only Stage 4 (1.67±0.33 points). There were differences in the quantitative evaluation of staging on Day 30. In Group 1 (POCS), it was 5.00±0.00 points in average while in Group 2 (POIS) it was insignificantly lower (4.67±0.33 points). Differences between healing stage criteria were statistically significant in the precision technique sutures versus DASS (P<0.05). We did not find a statistically significant difference in the healing process between the two types of anastomoses formed by the precision technique. In contrast to DASS, the precision technique sutures do not deform a bowel lumen. Thus, the use of microsurgical techniques is the preferred method for forming IA.