Morphogenesis in Cell and Tissue Cultures

Pua, Eng‐Chong

doi:10.1007/978-94-015-9253-6_10

Cited by 19 publications

(11 citation statements)

References 243 publications

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“…Evidence for the regulatory role of ethylene in shoot regeneration and SE has been well documented (Pua, 1999;Pua and Gong, 2004). High levels of ethylene accumulated in culture have been shown to be inhibitory to shoot regeneration of various Brassica members, which can be overcome by inhibition of ethylene synthesis or action by addition of ethylene inhibitors such as AVG or AgNO 3 in the medium.…”

Section: Discussionmentioning

confidence: 98%

Expression of glutathione-S-transferase and its role in plant growth and development in vivo and shoot morphogenesis in vitro

et al. 2005

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The enzymes glutathione-S-transferases (GSTs, E.C.2.5.1.18) have been associated with detoxification of xenobiotics, limiting oxidative damage and other stress responses in plants. In this study, we report the isolation of a mustard gene, BjGSTF2, homologous to the phi class GSTs and changes in plant growth in vivo and shoot regeneration in vitro were related to GST expression. GST transcripts accumulated differentially in mustard organs, where transcript was most abundant in root. Tissues incubated at high temperature or in the presence of exogenous H2O2, HgCl2, 1-aminocyclopropane-1-carboxylate, salicylic acid and paraquat upregulated GST expression, whereas spermidine was inhibitory. To investigate the in vivo function of GST, transgenic Arabidopsis thalianaplants expressing sense (GST-S6), antisense (GST-A4) and double-stranded BjGSTF2 (GST-DS1) RNAs were generated. GST-S6 was shown to flower two days earlier and was relatively more tolerant to HgCl2 and paraquat, whereas GST-DS1 with least stress tolerance flowered one week later compared to WT and GST-A4. In shoot regeneration response, tissues originated from GST-S6 were highly regenerative, whereas no shoot regeneration was observed in GST-DS1 tissues after 30 days of culture. Results of this study provide the evidence showing that GST plays a role in plant growth and development in vivo and shoot regeneration in vitro.

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Section: Discussionmentioning

confidence: 98%

Expression of glutathione-S-transferase and its role in plant growth and development in vivo and shoot morphogenesis in vitro

et al. 2005

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“…Various effects of ethylene on tissue culture have been reported (Biddington 1992;Pua 1999) with ethylene having either inhibitory or stimulatory effects depending on its concentration, and that there is an optimum and a limiting level of concentration in each case (Hatanaka et al 1995;El Meskaoui and Tremblay 2001;Chatfield and Raizada 2008). Furthermore, one additional difficulty in such studies is that released ethylene can either activate or inhibit its production autocatalytically (Arigita et al 2005).…”

Section: Discussionmentioning

confidence: 97%

“…The quantity accumulated depends on the production rate of ethylene in the tissues and the gas exchange rates in the cultures; although the culture flasks do allow exchange of ethylene, its production frequently exceeds its loss, resulting in accumulation in the culture flasks (George 1993;Pua 1999).…”

Section: Introductionmentioning

confidence: 99%

Ethylene and polyamine interactions in morphogenesis of Passiflora cincinnata: effects of ethylene biosynthesis and action modulators, as well as ethylene scavengers

et al. 2010

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Ethylene is a plant hormone that is of fundamental importance to in vitro morphogenesis, but in many species, it has not been thoroughly studied. Its relationship with polyamines has been studied mainly because the two classes of hormones share a common biosynthetic precursor, S-adenosylmethionine (SAM). In order to clarify whether competition between polyamines and ethylene influences in vitro morphogenetic responses of Passiflora cincinnata Mast., a climacteric species, different compounds were used that act on ethylene biosynthesis and action, or as ethylene scavengers. Treatment with the ethylene inhibitor, aminoethoxyvinylglycine (AVG) caused a greater regeneration frequency in P. cincinnata, whereas treatment with the ethylene precursor, 1-aminocyclopropane-1-carboxylic-acid (ACC) lessened regeneration frequencies. The data suggested that levels of polyamines and ethylene are not correlated with morphogenic responses in P. cincinnata. It was ascertained that neither the absolute ethylene and polyamine levels, nor competition between the compounds, correlated to the obtained morphogenic responses. However, sensitivity to, and signaling by, ethylene appears to play an important role in differentiation. This study reinforces previous reports regarding the requirement of critical concentrations and temporal regulation of ethylene levels for morphogenic responses. Temporal regulation also appeared to be a key factor in competition between the two biosynthetic pathways, without having any effects on morphogenesis. Further studies investigating the silencing or overexpression of genes related to ethylene perception, under the influence of polyamines in cell differentiation are extremely important for the complete understanding of this process.

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“…The interaction between PAs and ethylene occurs at different levels, but with contrasting reports (Minocha et al 1991;Matilla 1996;Pua 1999;Crozier et al 2000). Moreover, PAs and ethylene exert opposing physiological effects on senescence and fruit ripening (Winner and Apelbaum 1986).…”

Section: Introductionmentioning

confidence: 87%

“…They are interrelated through both their biosynthetic routes as well as their physiological roles (Alvarez et al 2003). Several studies have analyzed the relationship between different morphogenic capacities within the same (Pua 1999;El Meskaoui and Tremblay 2001;Shoeb et al 2001;Li and Burritt 2003;Zapata et al 2004;Hu et al 2006) or different (Marino et al 2008) species using various indicators other than polyamine and ethylene levels.…”

Section: Introductionmentioning

confidence: 99%

Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor

Dias

Santa–Catarina

Ribeiro

et al. 2009

Plant Cell Tiss Organ Cult

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Levels of ethylene and polyamines (PAs) were measured during organogenesis of hypocotyl explants of two species of passion fruit (Passiflora cincinnata Masters and Passiflora edulis Sims f. flavicarpa Degener 'FB-100') to better understand the relationships of these regulators and their influence on cell differentiation and morphogenesis. Moreover, histological investigation of shoot ontogenesis was conducted to characterize the different events involved in cell redifferentiation and regulation of PA and ethylene levels. A delay was observed in morphogenic responses of P. edulis f. flavicarpa as compared to P. cincinnata, and these changes coincided with production of elevated levels of polyamine and ethylene levels. During differentiation, cells showed high rates of expansion and elongation, and high ethylene levels were associated with high PA levels, suggesting that the two biosynthesis pathways were highly regulated. Moreover, their interaction might be an important factor for determining cell differentiation. The addition of PAs to the culture medium did not promote organogenesis; however, the incorporation of the PA inhibitor methylglyoxal bisguanylhydrazone in the culture medium reduced shoot bud differentiation, suggesting the need to maintaining a minimum level of PAs for morphogenic events to take place.

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Morphogenesis in Cell and Tissue Cultures

Cited by 19 publications

References 243 publications

Expression of glutathione-S-transferase and its role in plant growth and development in vivo and shoot morphogenesis in vitro

Expression of glutathione-S-transferase and its role in plant growth and development in vivo and shoot morphogenesis in vitro

Ethylene and polyamine interactions in morphogenesis of Passiflora cincinnata: effects of ethylene biosynthesis and action modulators, as well as ethylene scavengers

Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor

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