Morphogenesis of Penile Cavernous Fibrosis in Hypotestosteronemia: an Experimental Study

Kogan, M. I.; Todorov, S S; Popov, I; Popov, I. V.; Kulishova, M. A.; Ermakov, Alexey; Sizyakin, D. V.

doi:10.21886/2308-6424-2020-8-1-14-24

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“…Also, the time intervals of taking penile samples for these purposes vary depending on the CF model because of different dynamics of fibrogenesis caused by pathology in NO‐cGMP, TGF‐β, SHH, VEGF, and ROCK signaling pathways 6 . For instance, while CF occurs rapidly following cavernous nerve injury and castration within 2–4 weeks, 7,8 in diabetes mellitus, similar morphological changes can be observed within 6–12 weeks, 9,10 and in aging signs of CF could be detected throughout the entire life of laboratory animals 11 …”

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confidence: 99%

X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

et al. 2021

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Background: Current assessment methods of penile cavernous fibrosis in animal models have limitations due to the inability to provide complex and volume analysis of fibrotic alterations. Objective:The aim was to evaluate micro-computed tomography for assessment of cavernous fibrosis and compare it with histological, histochemical, immunohistochemical, and RT-PCR analysis. Materials and methods:A controlled trial was performed involving 25 New Zealand male rabbits with induced testosterone deficiency by orchidectomy. Penile samples were obtained before and after 7, 14, 21, and 84 days from orchidectomy. We consistently performed (a) gray value analysis of corpora cavernosa 3D models reconstructed after micro-computed tomography, (b) morphometry of smooth muscles/connective tissue ratio, collagen type I/III ratio, and area of TGF-beta-1 expression in corpora cavernosa, and (c) RT-PCR of TGF-beta-1 expression.Results: Micro-computed tomography allowed visualization of penile structures at a resolution comparable to light microscopy. Gray values of corpora cavernosa decreased from 1673 (1512-1773) on the initial day to 1184 (1089-1232) on the 21st day (p < 0.005). However, on the 84th day, it increased to 1610 (1551-1768). On 21st and 84th days, there was observed a significant decrease in smooth muscle/connective tissue ratio and a significant increase in collagen type I/III ratio (p < 0.05). TGF-beta1 expression increased on the 84th day according to immunohistochemistry (p < 0.005).RT-PCR was impossible to conduct due to the absence of RNA in obtained samples after micro-CT.Discussion and conclusions: Micro-computed tomography provided 3D visualization of entire corpora cavernosa and assessment of radiodensity alterations by gray value

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