Morphogenesis of the antenna of the male silkmoth. Antheraea polyphemus, III. Development of olfactory sensilla and the properties of hair-forming cells

Keil, Thomas A.; Steiner, Cornelia

doi:10.1016/0040-8166(91)90034-q

Cited by 41 publications

(21 citation statements)

References 63 publications

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“…This is particularly curious because there are typically two or three neurons present in each sensillum, all closely related, having derived from a common sensory mother cell (34). Furthermore, each neuron differs in its response to specific pheromone components (3,41,42).…”

Section: Discussionmentioning

confidence: 99%

“…Histological Analysis of Snmp-1 Expression in Antenna-The A. polyphemus antenna resembles a feather in form; branches are arrayed along a central stalk, and olfactory sensilla are organized in several parallel rows running the length of opposite sides of each branch (34). In situ hybridization studies showed Snmp-1 mRNA localized to specific cells within the male antenna (Fig.…”

Section: ϫ43mentioning

confidence: 99%

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Snmp-1, a Novel Membrane Protein of Olfactory Neurons of the Silk Moth Antheraea polyphemus with Homology to the CD36 Family of Membrane Proteins

Rogers

Sun

Lerner

et al. 1997

Journal of Biological Chemistry

201

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While olfactory neurons of silk moths are well known for their exquisite sensitivity to sex pheromone odorants, molecular mechanisms underlying this sensitivity are poorly understood. In searching for proteins that might support olfactory mechanisms, we characterized the protein profile of olfactory neuron receptor membranes of the wild silk moth Antheraea polyphemus. We have purified and cloned a prominent 67-kDa protein which we have named Snmp-1 (sensory neuron membrane protein-1). Northern blot analysis suggests that Snmp-1 is uniquely expressed in antennal tissue; in situ hybridization and immunocytochemical analyses show that Snmp-1 is expressed in olfactory neurons and that the protein is localized to the cilia, dendrites, and somata but not the axons. Snmp-1 mRNA expression increases significantly 1-2 days before the end of adult development, coincident with the functional maturation of the olfactory system. Sequence analysis suggests Snmp-1 is homologous with the CD36 protein family, a phylogenetically diverse family of receptor-like membrane proteins. CD36 family proteins are characterized as having two transmembrane domains and interacting with proteinaceous ligands; Snmp-1 is the first member of this family identified in nervous tissue. These findings argue that Snmp-1 has an important role in olfaction; possible roles of Snmp-1 in odorant detection are discussed.The antennae of silk moths are well known for their exquisite sensitivity to pheremonal odorants (1-3). Early reports demonstrated that the males of the wild silk moth Samia cynthia could locate a sex pheromone source over 2 miles away within several hours of their release (4). Studies of the silk moth Bombyx mori suggested that a single pheromone molecule was sufficient to activate olfactory neurons in the antenna (5). In insects, odors are detected by sensilla, small hair-like structures arrayed along the antennae. The sensilla are hollow, fluid-filled cuticular structures that contain the receptor cilia of olfactory neurons. Small holes penetrate through the wall of a sensillum, permitting entry of odor molecules; odorant-binding proteins are then thought to transport the odor molecules through the fluid-filled lumen to receptor proteins in the receptor membranes of the olfactory neurons (3, 6 -9).In searching for proteins that might support olfactory mechanisms, we characterized the protein profile of olfactory neuron receptor membranes of the wild silk moth Antheraea polyphemus. The morphology of the A. polyphemus antenna permits the relatively easy isolation of olfactory sensilla in a manner yielding olfactory receptor cilia as the only cellular component (10). This preparation is free of other parts of the olfactory neurons as well as of nonneuronal cells of the antenna, and it was previously used to identify a pheromonebinding membrane protein using a radiolabeled photoaffinity analog of the A. polyphemus sex pheromone (10). This protein co-migrated with bovine serum albumin on SDS gels (around 67 kDa) and appeared to be uniquely exp...

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Section: Discussionmentioning

confidence: 99%

Section: ϫ43mentioning

confidence: 99%

Snmp-1, a Novel Membrane Protein of Olfactory Neurons of the Silk Moth Antheraea polyphemus with Homology to the CD36 Family of Membrane Proteins

Rogers

Sun

Lerner

et al. 1997

Journal of Biological Chemistry

201

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“…Drosophila OSNs are encased in hair-like sensilla in the antennae and maxillary palps, with each sensillum containing the dendrites of one to four OSNs that are wrapped by sheath cells (18). The responses of native Drosophila OSNs to odors have traditionally been measured by electroantennogram (EAG) (19), which extracellularly measures the potentials across the entire antenna.…”

mentioning

confidence: 99%

Distinct signaling of Drosophila chemoreceptors in olfactory sensory neurons

Cao

Jing

Yang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

114

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In Drosophila, olfactory sensory neurons (OSNs) rely primarily on two types of chemoreceptors, odorant receptors (Ors) and ionotropic receptors (Irs), to convert odor stimuli into neural activity. The cellular signaling of these receptors in their native OSNs remains unclear because of the difficulty of obtaining intracellular recordings from Drosophila OSNs. Here, we developed an antennal preparation that enabled the first recordings (to our knowledge) from targeted Drosophila OSNs through a patch-clamp technique. We found that brief odor pulses triggered graded inward receptor currents with distinct response kinetics and current-voltage relationships between Or-and Ir-driven responses. When stimulated with long-step odors, the receptor current of Ir-expressing OSNs did not adapt. In contrast, Or-expressing OSNs showed a strong Ca 2+ -dependent adaptation. The adaptation-induced changes in odor sensitivity obeyed the Weber-Fechner relation; however, surprisingly, the incremental sensitivity was reduced at low odor backgrounds but increased at high odor backgrounds. Our model for odor adaptation revealed two opposing effects of adaptation, desensitization and prevention of saturation, in dynamically adjusting odor sensitivity and extending the sensory operating range.Drosophila | olfaction | chemoreceptor | sensory adaptation | OSN

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“…The neurons of A. polyphemus were detected 2 days after the larva molted to pupae, and the sensilla projections appeared after the third day (Keil and Steiner, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…The apical projection of the epidermal cells occurs in the pink eyed pupae, suggest cuticle deposition in this stingless bee. The cuticle that forms the antennal sensilla begins to be deposited by trichogen cells when the sensilla attain its final length (Kuhbandner, 1984;Keil and Steiner, 1991;Keil, 1997).…”

Section: Discussionmentioning

confidence: 99%

Development of antennal sensilla of Tetragonisca angustula Latreille, 1811 (Hymenoptera: Meliponini) during pupation

et al. 2016

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The antennal sensilla are sensory organs formed by a group of neurons and accessory cells, which allow perception of environmental cues, which play a role as mechanoreceptors and chemoreceptors. This study describes the post-embryonic development of the antennal sensilla of the stingless Tetragonisca angustula (Hymenoptera: Meliponini) workers. The development of the antennal sensilla begins in the transition stage of the pre-pupae to white-eyed pupae. The sensilla are completely developed at the black-eyed pupae stage, but they are covered by the old cuticle. The sensilla are exposed to the environment only in newly emerged workers of T. angustula, but it is possible that environmental stimuli can be recognized due to the pores in the old cuticle.Keywords: placoid sensilla, trichodea sensilla, stingless bee, pupal development.Desenvolvimento das sensilas antenais de Tetragonisca angustula latreille, 1811 (Hymenoptera: Meliponini) durante a pupação ResumoAs sensilas antenais são órgãos sensoriais formados por um conjunto de neurônios que captam estímulos ambientais e células acessórias, desempenhando as funções de mecanorreceptores e quimiorrecepterores. Este trabalho descreve o desenvolvimento pós-embrionário das sensilas antenais de operárias das abelhas sem ferrão Tetragonisca angustula (Hymenoptera: Meliponini). O desenvolvimento das sensilas tem início na transição de pré-pupa para pupa de olho branco e estão completamente desenvolvidas no estágio de pupa de olho preto, mas ainda estão cobertas pela cutícula velha. As sensilas estão completamente expostas em operárias recém-emergidas de T. angustula, mas é possível que estímulos ambientais sejam percebidos em estágios anteriores devido aos poros presentes na cutícula velha.Palavras-chave: sensila placoide, sensila tricoide, abelha sem ferrão, desenvolvimento pupal.

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Morphogenesis of the antenna of the male silkmoth. Antheraea polyphemus, III. Development of olfactory sensilla and the properties of hair-forming cells

Cited by 41 publications

References 63 publications

Snmp-1, a Novel Membrane Protein of Olfactory Neurons of the Silk Moth Antheraea polyphemus with Homology to the CD36 Family of Membrane Proteins

Snmp-1, a Novel Membrane Protein of Olfactory Neurons of the Silk Moth Antheraea polyphemus with Homology to the CD36 Family of Membrane Proteins

Distinct signaling of Drosophila chemoreceptors in olfactory sensory neurons

Development of antennal sensilla of Tetragonisca angustula Latreille, 1811 (Hymenoptera: Meliponini) during pupation

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