Morphogenesis of wheat calluses treated with Azospirillum lipopolysaccharides

Tkachenko, Oksana V.; Burygin, Gennady L.; Evseeva, Nina V.; Fedonenko, Yu. P.; Matora, L. Yu.; Lobachev, Yuriy Viktorovich; Shchyogolev, S. Yu.

doi:10.1007/s11240-021-02114-2

Cited by 4 publications

(7 citation statements)

References 38 publications

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“…Stronger negative values of the zeta-potential of supramolecular particles of LPS of strains O. cytisi IPA7.2 and O. quorumnocens T1Kr02 relative to LPS of the strain O. anthropi LMG3331 (−11.9 mV) [43] are explained by the presence of negatively charged groups: D-ManNAcA residues for O. cytisi IPA7.2 [32] and succinate residues for O. quorumnocens T1Kr02. In this case, the diameter of LPS micelles from O. quorumnocens T1Kr02 was significantly larger than the diameter of LPS micelles from bacteria of the genus Azospirillum with similar zeta-potential values [54].…”

Section: Discussionmentioning

confidence: 75%

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Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium Ochrobactrum quorumnocens T1Kr02, Containing d-Fucose Residues

Krivoruchko,

Zdorovenko,

Ivanova

et al. 2024

IJMS

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Lipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of Ochrobactrum quorumnocens T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical–chemical properties and biological activity of LPS were also investigated. The NMR analysis showed that the O-polysaccharide has the following structure: →2)-β-d-Fucf-(1→3)-β-d-Fucp-(1→. The structure of the periplasmic glucan coextracted with LPS was established by NMR spectroscopy and chemical methods: →2)-β-d-Glcp-(1→. Non-stoichiometric modifications were identified in both polysaccharides: 50% of d-fucofuranose residues at position 3 were O-acetylated, and 15% of d-Glcp residues at position 6 were linked with succinate. This is the first report of a polysaccharide containing both d-fucopyranose and d-fucofuranose residues. The fatty acid analysis of the LPS showed the prevalence of 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, lactobacillic, and 27-hydroxyoctacosanoic acids. The dynamic light scattering demonstrated that LPS (in an aqueous solution) formed supramolecular particles with a size of 72.2 nm and a zeta-potential of –21.5 mV. The LPS solution (10 mkg/mL) promoted the growth of potato microplants under in vitro conditions. Thus, LPS of O. quorumnocens T1Kr02 can be recommended as a promoter for plants and as a source of biotechnological production of d-fucose.

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Section: Discussionmentioning

confidence: 75%

“…Thus, for common wheat, it was shown that LPS of plant-growth-promoting rhizobacteria Azospirillum spp. improved seedling growth [59], increased grain yield [60], and benefited the yield of morphogenic tissue culture calluses [54]. The molecular mechanisms of interaction between bacterial LPS and plant cells are still poorly studied.…”

Section: Discussionmentioning

confidence: 99%

Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium Ochrobactrum quorumnocens T1Kr02, Containing d-Fucose Residues

Krivoruchko,

Zdorovenko,

Ivanova

et al. 2024

IJMS

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“…in sugarcane callus (Anand Kumar et al, 2004), rhizobacteria with saffron cormlet (Parray et al, 2015), H. seropedicae Z78 with oil palm callus and embryogenic callus (var. tenera and dura) (Lim et al, 2016a;Lim et al, 2016b), and A. brasilense with wheat callus (Tkachenko et al, 2021). All these studies reported that the selected PGPRs were found to be able to promote plant growth and further improve the efficiency of the micropropagation process by enhancing the cell division and proliferation.…”

Section: Artificial Symbiosis Of Pgpr and In Vitro Plant Culturementioning

confidence: 99%

“…tenera and dura ) ( Lim et al., 2016a ; Lim et al., 2016b ), and A . brasilense with wheat callus ( Tkachenko et al., 2021 ). All these studies reported that the selected PGPRs were found to be able to promote plant growth and further improve the efficiency of the micropropagation process by enhancing the cell division and proliferation.…”

Section: Artificial Symbiosis Of Pgpr and In Vitro ...mentioning

confidence: 99%

“…However, to date, less attention has been paid to the biotization processes of other in vitro culture materials such as callus and embryogenic callus. Not long ago, the potential of the PGPR to promote in vitro proliferation and differentiation of plant culture materials such as callus and embryogenic callus was reported in oil palm and wheat ( Lim et al., 2016a ; Lim et al., 2016b ; Tkachenko et al., 2021 ). Selected beneficial bacteria can accelerate the growth and development of in vitro plant culture via a highly sustainable and environmentally friendly approach.…”

Section: Introductionmentioning

confidence: 99%

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Biotization of in vitro oil palm (Elaeis guineensis Jacq.) and its plant-microbe interactions

et al. 2023

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Continuous discovery of novel in vitro plant culture practices is always essential to promote better plant growth in the shortest possible cultivation period. An alternative approach to conventional micropropagation practice could be achieved through biotization by inoculating selected Plant Growth Promoting Rhizobacteria (PGPR) into the plant tissue culture materials (e.g., callus, embryogenic callus, and plantlets). Such biotization process often allows the selected PGPR to form a sustaining population with various stages of in vitro plant tissues. During the biotization process, plant tissue culture material imposes developmental and metabolic changes and enhances its tolerance to abiotic and biotic stresses, thereby reducing mortality in the acclimatization and pre-nursery stages. Understanding the mechanisms is, therefore crucial for gaining insights into in vitro plant-microbe interactions. Studies of biochemical activities and compound identifications are always essential to evaluate in vitro plant-microbe interactions. Given the importance of biotization in promoting in vitro plant material growth, this review aims to provide a brief overview of the in vitro oil palm plant-microbe symbiosis system.

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