Morphogenetic and cellular reorientations induced by Agrobacterium rhizogenes (strains 1855, 2659 and 8196) on carrot, pea and tobacco

Bercetche, J.; Chriqui, Dominique; Adam, Suaad Elradi Hamid; David, C.

doi:10.1016/0168-9452(87)90053-7

Cited by 70 publications

(33 citation statements)

References 27 publications

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“…Plants regenerated from hairy roots are generally nonchimera since each hairy root line originates from a single cell (Bercetche et al 1987). However, they often exhibits various types of phenotypical alterations such as wrinkled leaves, shortened internodes, increase lateral branching, reduced apical dominance and better root yield than untransformed plants, which has been attributed to the fact that T-DNA from the bacterial Ri plasmid is transferred into infected plant cells and becomes stably integrated into the plant genome (Giri and Narasu 2000).…”

Section: Introductionmentioning

confidence: 99%

Iridoid and phenylethanoid glycoside production and phenotypical changes in plants regenerated from hairy roots of Rehmannia glutinosa Libosch.

Piątczak

Kuźma

Skała

et al. 2015

Plant Cell Tiss Organ Cult

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Spontaneous shoot regeneration was obtained from the hairy roots of Rehmannia glutinosa cultured in liquid woody plant medium for 4 weeks in darkness after induction by Agrobacterium rhizogenes strain A4. These shoots were proliferated on solid Murashige and Skoog (MS) medium in 300 mL glass jars and in liquid MS medium in a 5 L nutrient sprinkle bioreactor. These media were supplemented with 0.1 mg L -1 of indole-3-acetic acid (IAA) and 1.0 mg L -1 of 6-benzylaminopurine. All shoots became rooted on MS agar medium containing IAA or indole-3-butyric acid within 4 weeks. The plantlets were then transferred into soil and grew in a greenhouse. The transgenic nature of the plants was confirmed by polymerase chain reaction (PCR) and reverse-transcriptase PCR analyses. The pRi-regenerated transformants demonstrated a few phenotypical alterations. Production of six iridoid (catalpol, aucubin, loganin, catalposide, harpagide, harpagoside) and two phenylethanoid (verbascoside and isoverbascoside) glycosides was determined in shoots cultured in vitro and leaves and roots of 6-and 12-month-old plants by ultra high performance liquid chromatography. The shoot culture was able to synthesize low amounts of catalpol, verbascoside and isoverbascoside. Leaves and roots of pRi-regenerants accumulated similar (harpagoside, isoverbascoside), higher (catalposide, aucubin, harpagide, verbascoside) or lower (catalpol, loganin) levels of the metabolites in comparison with untransformed R. glutinosa plants and their content varied with the age of the plants and the organ analyzed. However, the increase in shoot and root biomass of transformed plants resulted in a significantly greater yield of all analyzed compounds.

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Section: Introductionmentioning

confidence: 99%

Iridoid and phenylethanoid glycoside production and phenotypical changes in plants regenerated from hairy roots of Rehmannia glutinosa Libosch.

Piątczak

Kuźma

Skała

et al. 2015

Plant Cell Tiss Organ Cult

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“…In tobacco, shoots have been regenerated from leaves following inoculation with the agropine-type strain A4, but their transformed nature was not determined (Tepfer, 1984). Normal plants without any mannopine were also recovered after infection of tobacco internodes with the strain 8196 (Bercetche et al, 1987). Recently, transformed shoots were reported to regenerate directly from decapitated rooted hypocotyls of Brassica napus after inoculation with Agrobacterium strains containing pRi 15834 (Damgaard & Rasmussen, 1991).…”

Section: Discussionmentioning

confidence: 97%

Direct regeneration of transformed plants from stem fragments of potato inoculated with Agrobacterium rhizogenes

Dobigny

Tizroutine

Gaisne

et al. 1996

Plant Cell Tiss Organ Cult

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Mannopine and cucumopine straios of Agrobacterium rhizogenes were used for genetic transformation in two cultivars of potato (Solanum tuberisum L.). An overnight pretreatment of internodes with c~-naphthaleneacetic acid prior to bacterial infection was found to strongly inhibit shoot formation. On the contrary, infection with bacterial strains enhanced the frequency of shoot formation, compared with the controls, except for the strain 15834 which completely inhibited shoot formation in both potato cultivars. Shoots developed directly from the upper part of both inoculated and control explants, at a frequency ranging from 1 to 5 shoots per fragment. Among 93 shoots regenerated, 9 were found to be opine positive, and exhibited an altered phenotype with shortened internodes. Histological study revealed that the transformed shoots developed directly from cells of the internode sections, and not from induced roots. When grown in an insect-proof tunnel, the transformed plants had both altered and normal phenotypes and were able to produce tubers.

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“…Berteche reported that, infection of carrot discs with Agrobacterium rhizogenes 8196 may occasionally lead to the bud neoformation at the inoculated surface. Transformed hairy roots are formed but the auxin-like symptoms at the cell and tissue levels were less striking after inoculation ( 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Infection of wounded dicotyledonous plants with Agrobacterium rhizogenes normally produces localized profuse root development. A segment of bacterial Ri plasmid can stably be integrated into a plant chromosome, and this transferred DNA (T-DNA) is root-inducing (12,4,8). Apical part of roots are the place of auxin synthesis which induces longitudinal growth and this "Auxin Induced Growth" is the result of the auxin treatment during the cell development.…”

Section: Introductionmentioning

confidence: 99%

Effects of Naphthalene Acetic Acid on Transformation Frequency of Potato and Tobacco ViaAgrobacterium Rhizogenes

Arican

Bajroviç

Gözükırmızı

1998

Biotechnology & Biotechnological Equipment

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Morphogenetic and cellular reorientations induced by Agrobacterium rhizogenes (strains 1855, 2659 and 8196) on carrot, pea and tobacco

Cited by 70 publications

References 27 publications

Iridoid and phenylethanoid glycoside production and phenotypical changes in plants regenerated from hairy roots of Rehmannia glutinosa Libosch.

Iridoid and phenylethanoid glycoside production and phenotypical changes in plants regenerated from hairy roots of Rehmannia glutinosa Libosch.

Direct regeneration of transformed plants from stem fragments of potato inoculated with Agrobacterium rhizogenes

Effects of Naphthalene Acetic Acid on Transformation Frequency of Potato and Tobacco ViaAgrobacterium Rhizogenes

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