Morphogenetic,  Physiological, and Biochemical Features of Lavandula angustifolia at Long-Term Micropropagation In Vitro

Yegorova, N.A.; Митрофанова, И.В.; Браилко, В.А.; Гребеннікова, О. А.; Paliy, А. Е.; Ставцева, И.В.

doi:10.1134/s1021443719010060

Cited by 14 publications

(7 citation statements)

References 11 publications

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“…We revealed an increase of multiplication index to 3-5 th subcultures during in vitro propagation for some other essential oil plants. The experiments with micropropagation of lavender in 9 subcultures showed that the number of shoots increased in all five studied cultivars up to the third subculture and decreased thereafter [20]. During the micropropagation of five mint cultivars and breeding samples during six subcultures, it was found that the multiplication index increased in 3-5 th passages, reaching 12.1-48.7 depending on the genotype and duration of cultivation [21].…”

Section: Discussionmentioning

confidence: 83%

Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivationin vitro

Yegorova

Stavtzeva

2020

E3S Web Conf.

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The development of explants of 12 cultivars and breeding samples of essential oil rose (obtained with the participation of Rosa damascena Mill., R. gallica L., R. alba L.) during long-term micropropagation was investigated. At the second multiplication stage 5 subcultures were carried out. Increase of the studied morphometric parameters of explants to 3-4th subcultures was established. In some genotypes, the maximum multiplication index was in the third subculture (сultivars ‘Raduga’, ‘Zolushka’, ‘Lada’, ‘Krymskaya Krasnaya’, ‘Lany’, ‘Vesna’ and samples N37-24, M215), while in others (сultivar ‘Kazanlykskaya’ and samples N138, G2168, N37-2) – in the fourth. The largest increase of the multiplication index in 3-4th subcultures compared to the first (3.0-4.8 times) was found in the cultivars ‘Lada’, ‘Lany’, ‘Raduga’, ‘Krymskaya Krasnaya’ and sample N37-24. In the fifth subculture the multiplication index decreased. However, in most cultivars and breeding samples it was higher than in the first subculture. The best morphogenetic potential was noted for сultivar ‘Raduga’ and samples G2168, N37-2, in which the multiplication index reached 14.2, 14.4 and 11.8, respectively. The minimal ability to propagation in vitro was in samples M215 and N138 – their multiplication index did not exceed 1.1 - 4.0.

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Section: Discussionmentioning

confidence: 83%

Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivationin vitro

Yegorova

Stavtzeva

2020

E3S Web Conf.

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“…Obtained in our experiments data on the change of the multiplication index during micropropagation of rose hybrids are comparable with the results for other plant species. An increase of multiplication index to the 3-5 th subcultures during in vitro propagation for some other essential oil plants, such as Origanum vulgare, Melissa officinalis [20], Lavandula angustifolia [21], was shown. For Vaccinium corymbosum it was also found that the fourth subculture gave the best results for multiplication [22].…”

Section: Resultsmentioning

confidence: 97%

Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

2021

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The aim of the work was to study the features of clonal micropropagation of essential oil rose interspecific hybrids obtained in embryo culture in vitro. Analysis of 12 crossing combinations demonstrated that the frequency of hybrid seedling formation in the embryo culture varied from 0 to 71.4%. For clonal micropropagation obtained in vitro seedlings were divided into stem segments with a node and cultivated on MS culture medium supplemented with 0.5 mg/l BAP and 0.1 mg/l GA3. During the multiplication of 13 hybrids (R. alba × R. damascena cv. ‘Kazanlykskaya’) in 2-6 subcultures, high variability of the multiplication index (1.8-18.5 depending on the genotype and passage) was revealed. This parameter was maximum in the 3-4th subcultures. The best ability to micropropagation showed hybrid No. 37-14. Microshoots were rooted in vitro on ½ MS medium, containing for different hybrids 0.5 or 1.0 mg/l NAA; frequency – up to 80.5-100.0%. However, in No. 37-2, 37-19 and 37-31 on four tested media, the number of shoots with roots was only 0-35.4%.

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“…To improve the regeneration frequency of the explant, the optimized concentration of BA (5.0 μM) was applied with auxin in a combined treatment. The synergistic effect of cytokinin and auxin is responsible for the control of main and lateral shoot formation (Bhatla, 2018 ; Yegorova et al, 2019 ). As observed, the combination of BA (5.0 μM) and NAA (0.5 μM) was found to be the optimal combination media for better growth and development.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Ahmad

Yadav²,

Shahzad³

et al. 2022

Front. Plant Sci.

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Casuarina equisetifolia is an important tree of the forest, cultivated in tropical and subtropical regions, providing fuelwood, land reclamation, dune stabilization, paper production, and nitrogen fixation. We have developed a systematic in vitro propagation protocol in C. equisetifolia using nodal segments (NS). Murashige and Skoog (MS) medium augmented with BA (5.0 μM) and NAA (0.5 μM) gave rise to a maximum of 32.00 ± 0.31 shoots per explant (S/E) with shoot length (SL) of 3.94 ± 0.02 cm, and a maximum of 70% regeneration potential (RP) was recorded after 8 weeks of post inoculation. For root induction, in vitro derived shoots were transferred to the nutrient medium consisting of a half-strength (½) MS medium augmented with 2.5 μM NAA, which produced a maximum of 12.68 ± 0.33 roots/shoot (R/S) with 3.04 ± 0.50 cm root length (RL) in 60% of culture after 6 weeks. Micropropagated plants with healthy shoots and roots were successfully acclimatized in vermicompost + garden soil + sand (1:2:1) and a maximum survival percentage of 95.1% was recorded. NS was taken from a 6-weeks-old in vitro derived plant of C. equisetifolia for synthetic seed production, and it was reported that CaCl2 · 2H2O (100 mM) + Na2-alginate (4%) resulted in clear and uniform beads. Furthermore, the maximum conversion of synthetic seeds into plantlets occurred over a period of 4 weeks of storage at 4°C. Scanning Electron Microscopy (SEM) revealed the formation of direct shoot buds without any intermediate callus formation. In addition, the chlorophyll and carotenoid contents of the direct regenerated and mother plant were compared. Similarly, RAPD and ISSR primers were used for genetic homogeneity assessment of the direct regenerated plants, where a total of 18 and 19, respectively, clear and reproducible bands with 100% monomorphism were recorded. The developed micropropagation protocol can certainly be used for large-scale multiplication and germplasm preservation of C. equisetifolia. It will also help in meeting the growing demands of C. equisetifolia in the forest industry.

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Morphogenetic, Physiological, and Biochemical Features of Lavandula angustifolia at Long-Term Micropropagation In Vitro

Cited by 14 publications

References 11 publications

Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivationin vitro

Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivationin vitro

Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

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