Morphogenic Aspects of Somatic Embryogenesis

Merkle, S. A.; Parrott, W. A.; Flinn, Barry S.

doi:10.1007/978-94-011-0485-2_5

Cited by 212 publications

(165 citation statements)

References 264 publications

(370 reference statements)

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“…Weatherhead et al (1978) demonstrated that 0.3% activated charcoal absorbs HMF and stimulates explant growth and induces a 200 to 300% increment in plantlet number. Another hypothesis for the beneficial effect of activated charcoal is the absorption of auxins liberated by the embryos to the medium during their development, which may interfere with embryo morphology and germination (Merkle et al, 1995). In Quercus suber L., we observed a significant increase of both embryo size and fresh weight due to activated charcoal, as well as a better control of repetitive embryogenesis in mature embryos.…”

Section: Effect Of Amino Acids and Activated Charcoal On Embryo Growthmentioning

confidence: 71%

“…During maturation, cell expansion and accumulation of reserves take place (Merkle et al, 1995), which implies both size and weight increment. In cork oak, this has been significantly promoted by the effect of activated charcoal added to the culture medium.…”

Section: Effect Of Amino Acids and Activated Charcoal On Embryo Growthmentioning

confidence: 99%

“…During maturation, cell expansion and storage substance accumulation takes place. The correct storage of reserve substances is crucial for fuelling the germination process (Merkle et al, 1995). Embryo weight increase is an indicator of embryo quality and a pre-requisite for successful germination (García-Martin et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Pintos¹,

Manzanera

Bueno³

2010

Ann. For. Sci.

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Abstract• Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos.• The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin.• Activated charcoal promoted a significant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more efficient control of secondary somatic embryogenesis during development. A significant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show significant differences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 • C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid.• We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the first regeneration of doubled-haploid plantlets in cork oak. • Le développement des deux embryogenèses gamétique et somatique a de nombreuses applications dans la foresterie clonale et l'amélioration génétique, par exemple en tant que propagation en masse de plants génétiquement améliorés et la production de lignées pures par régénération de plants haploïdes doublés d'embryons gamétiques. Mots-clés• L'objectif de ce travail était d'améliorer la croissance, la maturation et la régénération des plantules des embryons de chêne-liège (Quercus suber L.) des deux origines gamétique et somatique.• Le charbon actif a favorisé une augmentation significative de la croissance en termes de taille et de poids des embryons haploïdes doublés et somatiques, ainsi qu'un contrôle plus efficace de l'embryogenèse somatique secondaire au cours du développement. Une interaction significative a également été observée avec la nutrition aminoacide. Alors que certains acides aminés (c'est-à-dire, la glutamine, l'arginine ou l'asparagine) ne montrent pas de différences significatives avec les témoins, un mélange de ces trois acides aminés ou l'acide butyrique amino gamma a stimulé la croissance de l'embryon. Le taux de survie le plus élevé au cours de l'acclimatation des plantules d'origine à la fois somatique et haploïde doublé, a été obtenu lorsque les embryons ont été auparavant cultivés sur milieu de base à 3 % de saccharose et de 1 % de charbon actif pendant deux mois à 4 • C et ont germé sur un milieu complété par la 6-benzylaminopurine et ...

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Section: Effect Of Amino Acids and Activated Charcoal On Embryo Growthmentioning

confidence: 71%

Section: Effect Of Amino Acids and Activated Charcoal On Embryo Growthmentioning

confidence: 99%

See 1 more Smart Citation

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Pintos¹,

Manzanera

Bueno³

2010

Ann. For. Sci.

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“…Individual genotypes may also have unique requirements for optimal regeneration capacity. Such genotypic differences in embryogenic capacity might reflect current differences in the ability to activate key elements in the embryogenic pathway (Merkle et al, 1995). The use of PEG as an osmoticum in maturation media for somatic embryos has proven effective in increasing germination and conversion (Capuana and Debergh, 1997).…”

Section: Effect Of Carrier Substrate In Acclimatization Of Plantletsmentioning

confidence: 99%

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.

Bajpai

Kalim

Chandra

et al. 2016

Braz. arch. biol. technol.

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“…These phases are characterized by distinct biochemical and molecular events (Suprasanna and Bapat, 2005). Thus, the more closely the pattern of somatic embryo gene expression matches that of zygotic embryos, the greater the chance of obtaining highly efficient regenerative systems (Merkle et al 1995). There is, nevertheless, no international agreement amongst authors regarding the terminology for each step and the associated species.…”

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confidence: 99%