Morphologic alterations in drug sensitive vs. drug resistant cells due to cytostatic application

Examination by laser scanning confocal fluorescence imaging microscopy of the subcellular localisation of anthracyclines in parent and multidrug resistant cell lines

“…In general, studies on drug accumulation and efflux involve measurement of the whole cell drug content, which is essentially representative of the averaged drug accumulated per cell. However, several reports have indicated distinct patterns of intracellular accumulation and localisation for the anthracyclines (Dietel & Seidel, 1990;Willingham et al, 1986;Keizer et al, 1989). Such studies have been facilitated by the fact that anthracyclines are highly fluorescent molecules and their presence in cellular material can be visualised by fluorescence microscopy.…”

Uptake of lipiodol-cytotoxics conjugates by hepatocellular carcinoma cells

“…5 Using fluorescence microscopy, highly fluorescent molecules such as the anthracyclines have been observed to exhibit distinct patterns of intracellular accumulation and localisation. 6,7 Doxorubicin and daunorubicin have been shown to be localised in the nucleus of many cultured cells. 8,9 Confocal laser scanning microscopy (CLSM) enables detection and scanning of an intracellular location after fluorescence illumination by a laser light source such as Krypton/Argon laser of the same spot, with precise vertical and lateral resolution and the elimination of epifluorescence, including out-of-focus blur.…”