Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Shirazi, Mohammad Sadra; Heidari, Banafsheh; Shirazi, Abolfazl; Zarnani, Amir Hassan; Jeddi-Tehrani, Mahmood; Rahmati-Ahmadabadi, Maryam; Naderi, Mohammad; Behzadi, Bahareh; Farab, Moretza; Sarvari, Ali; Borjian-Boroujeni, Sara; Akhondi, Mohammad Mehdi

doi:10.1007/s10815-014-0301-5

Cited by 8 publications

(3 citation statements)

References 59 publications

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“…Expression of proliferation-related proteins was evaluated using the immunocytochemical staining [ 37 ]. In brief, 5x10 5 GES-1 cells were cultured on glass cover slips overnight, and co-cultured with 1×10 8 H. pylori or H. pylori cells for 24 h. The cells were fixed with 4% paraformaldehyde in PBS for 15 min followed by permeabilization with 0.5% Triton X-100 in PBS for 3 min.…”

Section: Methodsmentioning

confidence: 99%

NaCl pretreatment attenuates H.pylori-induced DNA damage and exacerbates proliferation of gastric epithelial cells (GES-1)

Yan

Hou

et al. 2015

Infect Agents Cancer

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BackgroundBoth H. pylori infection and high salt (NaCl) diet are risks of gastric cancer, however, the interaction pattern of the two is not very clear. Our objective was to investigate the effects of NaCl-pretreated H. pylori on DNA damage and proliferation of gastric epithelial cell (GES-1).MethodsGES-1 cells were co-cultured with H.pylori or NaCl-pretreated H. pylori (with 30% NaCl) for 24 h. The morphological changes of all cells were observed by inverted phase contrast microscopy and transmission electron microscopy. Oxidative DNA damage was examined by immunofluorescence. Alterations in mitochondrial membrane potential and apoptosis rate were detected by flow cytometry and western blot, and expression of Ki-67, PCNA and P21 were evaluated using the immunocytochemical staining.ResultsGES-1 cells co-cultured with NaCl-pretreated H.pylori exhibited morphological changes and oxidative DNA damage. Although no significant disruption of the mitochondrial membrane potential (ΔΨm) and apoptotic rate were observed compared with control groups, there were significant decreased in Bax and Caspase3 proteins and increased in Bcl-2 protein in GES-1 cells infected with H. pylori30 when compared with GES-1 cells cultured with H. pylori. In addition, we found a proliferative effect on GES-1 cells with an increased expression of Ki-67 and PCNA as well as a decreased p21 expression, through which the cells may acquire the potential for malignant transformation.ConclusionNaCl-pretreated H. pylori possessed the ability to cause cell injury and promote proliferation in gastric epithelial cells.

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Section: Methodsmentioning

confidence: 99%

NaCl pretreatment attenuates H.pylori-induced DNA damage and exacerbates proliferation of gastric epithelial cells (GES-1)

Yan

Hou

et al. 2015

Infect Agents Cancer

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“…In our case of alpaca spermatogonial cells, they were co‐cultured with Sertoli cells, allowing a suitable microenvironment to be produced for the proliferation of SSCs. On the other hand, the importance of growth factors has been recognised in the self‐renewal of stem cells (Kubota et al, ; Shirazi et al, ). Therefore, the presence of Sertoli cells in their secretion function, as well as the supplementation with human milk serum, would be providing the necessary factors for the proliferation of SSC in our in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed withDolichos biflorusby flow cytometry

et al. 2019

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Spermatogonial stem cell (SSC) is known for its self‐renewal capacity. We have studied the in vitro proliferation of isolated SSC from adult alpaca (Vicugna pacos) testes. A total of 107 samples were evaluated of which 31 were evaluated at baseline, 36 were cultivated in DMEM and 40 in STEMPRO media. Half of the cultivated samples was analysed after 14 days, and the rest after 21 days. Round cell subpopulations were identified with FITC‐DBA by flow cytometry: strongly positive DBA (sDBA+) as SSC, weakly positive DBA (wDBA+) as in early differentiation and negative DBA as differentiated. At the beginning, 4.16% of the cells were SSC, 37.61% wDBA+ while 54.12% were DBA−. After 14 days, 42.28% of SSC, 44.68% wDBA+ and 11.07% DBA− were found in DMEM while 47.09% of SSC, 32.57% wDBA+ and 18.48% DBA− in STEMPRO. After 21 days 38.66% were SSC, 52.78% wDBA and 7.65% DBA− in DMEM and on STEMPRO media 47.92% SSC, 44.20% wDBA+, 4.93% DBA−. There is a significant difference between the number of initial and SSC cultivated, as well as between DBA− (p < 0.05), while there is no significant difference between the wDBA+ (p > 0.05). Our results suggest that both culture media are appropriate for the in vitro proliferation of alpacas SSC.

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“…In 2000, glial cell line-derived neurotrophic factor (GDNF) was demonstrated to regulate the fate of undifferentiated spermatogonia in mice, 70 even though it was initially identified as a neurotrophic factor. Currently, GDNF and fibroblast growth factor 2 (FGF2) are known to be essential factors for the maintenance of SSC self-renewal in culture, 71 72 73 with GDNF having been shown to regulate the fate of SSCs in a dose-dependent manner. 70 Wang et al .…”

Section: Culture Of Sscsmentioning

confidence: 99%

Recent advances in isolation, identification, and culture of mammalian spermatogonial stem cells

Hao

Ren

et al. 2022

Asian Journal of Andrology

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Continuous spermatogenesis depends on the self-renewal and differentiation of spermatogonial stem cells (SSCs). SSCs, the only male reproductive stem cells that transmit genetic material to subsequent generations, possess an inherent self-renewal ability, which allows the maintenance of a steady stem cell pool. SSCs eventually differentiate to produce sperm. However, in an in vitro culture system, SSCs can be induced to differentiate into various types of germ cells. Rodent SSCs are well defined, and a culture system has been successfully established for them. In contrast, available information on the biomolecular markers and a culture system for livestock SSCs is limited. This review summarizes the existing knowledge and research progress regarding mammalian SSCs to determine the mammalian spermatogenic process, the biology and niche of SSCs, the isolation and culture systems of SSCs, and the biomolecular markers and identification of SSCs. This information can be used for the effective utilization of SSCs in reproductive technologies for large livestock animals, enhancement of human male fertility, reproductive medicine, and protection of endangered species.

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Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Cited by 8 publications

References 59 publications

NaCl pretreatment attenuates H.pylori-induced DNA damage and exacerbates proliferation of gastric epithelial cells (GES-1)

NaCl pretreatment attenuates H.pylori-induced DNA damage and exacerbates proliferation of gastric epithelial cells (GES-1)

In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed withDolichos biflorusby flow cytometry

Recent advances in isolation, identification, and culture of mammalian spermatogonial stem cells

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