Morphologic, cytometric and functional characterization of the common octopus (Octopus vulgaris) hemocytes

Castellanos-Martínez, Sheila; Prado-Álvarez, María; Lobo‐da‐Cunha, Alexandre; Azevedo, Catarina M; Gestal, Camino

doi:10.1016/j.fsi.2013.03.190

Cited by 6 publications

(20 citation statements)

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“…This species is host to Schistosoma mansoni which causes schistosomiasis in humans, so the relationship between snails and larvae of trematodes is the key for the current understanding of the hemocyte functions in the gastropoda (Littlewood, Bray 2001). According to Castellanos-Martínez et al (2014) there are two classes of hemocytes: granulated and agranulated lymphocytes. Research carried out later on this species indicated three types of hemocytes, classified as large, medium and small hemocytes (Matricon-Gondran, Letocart 1999).…”

Section: Discussionmentioning

confidence: 99%

Seasonally dependent morphological variations of circulating hemocytes in Helix pomatia

2018

EEB

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Total number and types of hemocytes were analysed in hemolymph of Roman snail (Helix pomatia L.). Significant differences in the number and types of hemocytes during the season at different locations were identified. The average number of hemocytes ranged from 154 to 288 per 10 μL of hemolymph. The highest number of hemocytes was recorded in snails at higher altitudes. Significant intergroup differences between all sites were identified. The most numerous hemocytes were granulocytes, followed by agranulocytes, while hyalinocytes were the least common in the post-hibernation period. Post hoc analysis showed certain intragroup differences for most locations except for comparison between wet habitat and habitats with anthropogenic influence, and between wet habitats and urban place with wet vegetation. A large number of transformed hemocytes in the encapsulated phase were identified in snail hemolymph infected with flat worms. Our results represent the first record of data for the number of hemocytes in molluscs and the first data for hemocytes of Helix pomatia. Further investigations and development of a uniform classification of hemocytes and their role in the immune response would be necessary in the future.

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Section: Discussionmentioning

confidence: 99%

Seasonally dependent morphological variations of circulating hemocytes in Helix pomatia

2018

EEB

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“…Blood samples could be obtained from the cephalic aorta (following Castellanos‐Martínez et al . ,) but it will be necessary to develop molecular tools for detecting male and female hormones from available samples without sacrificing octopuses (Larson & Anderson ).…”

Section: Larviculture Challengesmentioning

confidence: 99%

“…All these defence strategies have recently been studied in S. officinalis and O. vulgaris (Castellanos‐Martínez et al . ,; Gestal & Castellanos‐Martínez ), which opens other study strategy to determine the effect of diets, productive management and stress, on the innate immunity of cephalopods.…”

Section: New Metabolic and Molecular Research Toolsmentioning

confidence: 99%

Early life stage bottlenecks of carnivorous molluscs under captivity: a challenge for their farming and contribution to seafood production

Uriarte

Astorga

Navarro

et al. 2018

Reviews in Aquaculture

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This work brings together the view of different specialists in the areas of larviculture, physiology, ecology, nutrition and animal health, regarding how to deal with the aquaculture farming of species with complex life cycles in a multidisciplinary way, using as models the octopus and the muricid C. concholepas, with the aim of reducing the gap between the experimental and the industrial culture of species that are relevant for the diversification of aquaculture, particularly in Chile. Although these species are similar in their difficulty to reach the terminal planktonic phase prior to juvenile, they differ in the bottlenecks they have to overcome to reach it. Relevant aspects of study to achieve juvenile production from early life stages rearing, whether for repopulation or for ongrowing, are as follows: (i) Replacement or supplementation of live diets with inert diets to achieve significant survival values over the first stages of life. (ii) Physiological approaches to establish cultivation conditions evaluating the individual responses to several rearing conditions, specially the interaction between temperature, dissolved oxygen and acidity. Studies of urgent character due to the global warming scenario. (iii) Genomic studies associated with the effect of ontogenetic development, environment, health and nutrition on gene expression to understand, in an integrated way, the key processes for the development and growth of immature stages. (iv) Studies on the control of reproduction, the quality control of the ova, the genetic structure of reproductive populations and the characterization of diseases are also necessary to achieve efficient hatchery technologies.

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“…Haemocytes are crucial in the cephalopod immune response [51,52,53]. Although these cells are vital to maintain the wellbeing in all different cephalopod species, the studies conducted about haemocytes are limited, and most of the observations have been performed on octopus species [45,50,54,55,56,57,58] with few descriptions available on Euprymna scolopes [59] and Sepia officinalis [46,60,61]. In one of the first studies of characterization based on morphology and immune capability of cells, Stuart [49], identified phagocytic and non-phagocytic "leucoblastic cells" in common octopus.…”

Section: Type and Number Of Haemocytes In The Cephalopods Haemolymphmentioning

confidence: 99%

Understanding the cephalopod immune system based on functional and molecular evidence

Gestal

Castellanos-Martínez

2015

Fish & Shellfish Immunology

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Cephalopods have the most advanced circulatory and nervous system among the mollusks. Recently, they have been included in the European directive which state that suffering and pain should be minimized in cephalopods used in experimentation. The knowledge about cephalopod welfare is still limited and several gaps are yet to be filled, especially in reference to pathogens, pathologies and immune response of these mollusks. In light of the requirements of the normative, in addition to the ecologic and economic importance of cephalopods, in this review we update the work published to date concerning cephalopod immune system. Significant advances have been reached in relation to the characterization of haemocytes and defensive mechanisms comprising cellular and humoral factors mainly, but not limited, in species of high economic value like Sepia officinalis and Octopus vulgaris. Moreover, the improvement of molecular approaches has helped to discover several immune-related genes/proteins. These immune genes/proteins include antimicrobial peptides, phenoloxidases, antioxidant enzymes, serine protease inhibitor, lipopolysaccharide-induced TNF-α factor, Toll-like receptors, lectins, even clusters of differentiation among others. Most of them have been found in haemocytes but also in gills and digestive gland, and the characterization as well as their precise role in the immune response of cephalopods is still pending to be elucidated. The assessment of immune parameters in cephalopods exposed to contaminants is just starting, but the negative impact of some pollutants on the immune response of the common octopus has been reported. This review summarizes the current status of our knowledge about the cephalopod immune system that seems to be far from simply. On the contrary, the advances gained to date point out a complex innate immunity in cephalopods.

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Morphologic, cytometric and functional characterization of the common octopus (Octopus vulgaris) hemocytes

Cited by 6 publications

References 0 publications

Seasonally dependent morphological variations of circulating hemocytes in Helix pomatia

Seasonally dependent morphological variations of circulating hemocytes in Helix pomatia

Early life stage bottlenecks of carnivorous molluscs under captivity: a challenge for their farming and contribution to seafood production

Understanding the cephalopod immune system based on functional and molecular evidence

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