Morphologic study of three collagen materials for body wall repair

Soiderer, Emily E; Lantz, Gary C.; Kazacos, Evelyn A.; Hodde, Jason P.; Wiegand, Ryan E.

doi:10.1016/s0022-4804(03)00352-4

Cited by 67 publications

(61 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A variety of materials exist, each with their own advantages and corresponding complications. 26,34,35 In tissue engineering applications, some investigators have reported the successful use of various cells and SIS to restore defects. 36,37 In the present study, we explored the feasibility of seeding tenocytes onto SIS to reconstruct abdominal wall defects in a rat model.…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Song

Peng

Liu

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

The repair of abdominal wall defects following surgery remains a difficult challenge. Although multiple methods have been described to restore the integrity of the abdominal wall, there is no clear consensus on the ideal material for reconstruction. This study explored the feasibility of in vivo reconstruction of a rat model of an abdominal wall defect with a composite scaffold of tenocytes and porcine small intestinal submucosa (SIS). In the current study, we created a 2 · 1.5 cm abdominal wall defect in the anterolateral abdominal wall of SpragueDawley rats, which were assigned into three groups: the cell-SIS construct group, the cell-free SIS scaffold group, and the abdominal wall defect group. Tenocytes were obtained from the tendons of rat limbs. After isolation and expansion, cells (2 · 10 7 /mL) were seeded onto the three-layer SIS scaffolds and cultured in vitro for 5 days. Cell-SIS constructs or cell-free constructs were implanted to repair the abdominal wall defects. The results showed that the tenocytes could grow on the SIS scaffold and secreted corresponding matrices. In addition, both scaffolds could repair the abdominal wall defects with no hernia recurrence. In comparison to the cell-free SIS scaffold, the composite scaffold exhibited increased vascular regeneration and mechanical strength. Furthermore, following increased time in vivo, the mechanical strength of the composite scaffold became stronger. The results indicate that the composite scaffold can provide increased mechanical strength that may be suitable for repairing abdominal wall defects.

show abstract

Section: Discussionmentioning

confidence: 99%

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Song

Peng

Liu

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Bandage changes and planimetry were performed on days 0, 2,4,6,9,12,15,18,21,24,28,31,35,38,42,45,48,52,55,62, and 69. Biopsies for histopathologic evaluation were taken on days 0, 2, 4, 6, 9, 15, 21, and 31.…”

Section: Methodsmentioning

confidence: 99%

“…34 There are numerous, mostly experimental, reports on the application of ECM bioscaffolds in the repair and reconstitution of many soft tissue structures with variable success. Tissues include the lower urinary tract, [35][36][37] laryngeal cartilage, 38 esophagus, 39 abdominal wall, [40][41][42][43][44][45] diaphragm, 46,47 dura mater, 48 blood vessels, [49][50][51] tendons, [52][53][54][55][56] ligaments, 57,58 fascia, 59 menisci, [60][61][62][63] and bone. 64 Both commercially available ECM matrices on the veterinary market are promoted as implantable products to accelerate wound healing in dogs and cats.…”

mentioning

confidence: 99%

Effect of Porcine Small Intestinal Submucosa on Acute Full‐Thickness Wounds in Dogs

et al. 2008

View full text Add to dashboard Cite

Objective-To evaluate the effects of porcine small intestinal submucosa (PSIS) on the healing of full-thickness wounds in dogs, specifically the appearance of granulation tissue, percent epithelialization and contraction, histologic variables of inflammation and repair, and aerobic culture results. Study Design-Prospective, controlled, experimental study. Animals-Purpose-bred, female dogs (n ¼ 10). Methods-Wounds were created bilaterally on the trunk; 1 side as a control and 1 treated with PSIS. First appearance of granulation tissue was recorded. Total wound area, open wound area, and epithelialized area were measured at 21 time points-wound contraction and percent epithelialization were calculated. Aerobic cultures were taken at 4 time points and wound biopsies at 8. Histologic features were graded into an Acute Inflammation Score and Repair Score. Results-There was no difference in first appearance of granulation tissue between PSIS-treated and control wounds. Wound contraction was significantly faster in control wounds as was percent epithelialization after day 21. Histologic Acute Inflammation Scores were significantly higher in PSIS-treated wounds compared with control wounds on days 2 and 6. There were no differences in Histologic Repair Scores between PSIS-treated and control wounds or in aerobic culture results. Conclusion-Wounds treated with PSIS contract more slowly, epithelialize less, and have more pronounced acute inflammation after implantation than control wounds. Clinical Relevance-Acute, full-thickness wounds in dogs do not benefit from treatment with PSIS. r

show abstract

“…In choosing appropriate material as the scaffold for use in , and Feng-Huei Lin corneal tissue engineering, the effects of corneal cell proliferation and migration on the material need to be considered in advance (Orwin and Hubel, 2000). Collagenbased materials are ideal for soft-tissue engineering because of their superior biocompatibility (Soiderer et al, 2004). In this study, we present the design, development, and preliminary evaluation of an acellular, decalcified fabricated scaffold that fits the need for the design of current corneal prostheses.…”

Section: Introductionmentioning

confidence: 99%

A new fish scale-derived scaffold for corneal regeneration

Lin

Ritch²,

Lin³

et al. 2010

eCM

View full text Add to dashboard Cite

The purpose of this study is to develop a novel scaffold, derived from fish scales, as an alternative functional material with sufficient mechanical strength for corneal regenerative applications. Fish scales, which are usually considered as marine wastes, were acellularized, decalcified and fabricated into collagen scaffolds. The microstructure of the acellularized scaffold was imaged by scanning electron microscopy (SEM). The acellularization and decalcification treatments did not affect the naturally 3-dimentional, highly centrally-oriented micropatterned structure of the material. To assess the cytocompatibility of the scaffold with corneal cells, rabbit corneal cells were cultured on the scaffold and examined under SEM and confocal microscopy at different time periods. Rapid cell proliferation and migration on the scaffold were observed under SEM and confocal microscopy. The highly centrallyoriented micropatterned structure of the scaffold was beneficial for efficient nutrient and oxygen supply to the cells cultured in the three-dimensional matrices, and therefore it is useful for high-density cell seeding and spreading. Collectively, we demonstrate the superior cellular conductivity of the newly developed material. We provide evidences for the feasibility of the scaffold as a template for corneal cells growth and migration, and thus the fish scale-derived scaffold can be developed as a promising material for tissue-engineering of cornea.

show abstract

Morphologic study of three collagen materials for body wall repair

Cited by 67 publications

References 29 publications

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Effect of Porcine Small Intestinal Submucosa on Acute Full‐Thickness Wounds in Dogs

A new fish scale-derived scaffold for corneal regeneration

Contact Info

Product

Resources

About