Morphological alterations in rat peritoneal mast cells by stem cell factor

Kim, H. M.; Shin, Hye‐Young; Lee, E. H.

doi:10.1046/j.1365-2567.1998.00499.x

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…Lorentz et al demonstrated that in response to stimulation with SCF, human intestinal mast cells preferentially spread on fibronectin coated tissue culture plates and this cell spreading was accompanied by formation of pseudopodia. SCF was also shown to induce morphological alterations in rat peritoneal mast cells as demonstrated by Kim et al. We were not, however, able to show any effect of SCF in mediating mast cell adhesion or spreading.…”

Section: Discussioncontrasting

confidence: 61%

Mechanism of mast cell adhesion to human tenocytes in vitro

Behzad

Tsai

Nassab

et al. 2014

Journal Orthopaedic Research

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Mast cells and fibroblasts are two key players involved in many fibrotic and degenerative disorders. In the present study we examined the nature of binding interactions between human mast cells and tendon fibroblasts (tenocytes). In the mast cellfibroblast co-culture model, mast cells were shown to spontaneously bind to tenocytes, in a process that was partially mediated by a5b1 integrin receptors. The same receptors on mast cells significantly mediated binding of these cells to tissue culture plates in the presence of tenocyte-conditioned media; the tenocyte-derived fibronectin in the media was shown to also play a major role in these binding activities. Upon binding to tenocytes or tissue culture plates, mast cells acquired an elongated phenotype, which was dependent on a5b1 integrin and tenocyte fibronectin. Additionally, tenocyte-derived fibronectin significantly enhanced mRNA expression of the adhesion molecule, THY1, by mast cells. Our data suggests that a5b1 integrin mediates binding of mast cells to human tenocyte and to tenocyte-derived ECM proteins, in particular fibronectin. Decreased collagen production and increased MMP activities have been detected in tendon biopsies from patients with tendinopathy.3 To date, only a handful of studies have investigated the possible contribution of mast cells in tendinopathy. Mast cells have previously been reported to directly communicate with fibroblasts via gap junction intercellular communications, 4,5 and the SCF/c-kit receptor interaction has been shown to mediate adhesion of mouse mast cells to 3T3 fibroblasts. 6 It is very likely that due to heterogeneity in fibroblast and mast cell populations, different receptors are involved in the process of adhesion, and understanding the exact nature of these specific cell-cell interactions could help delineate these connections.In this study, we utilized an in vitro cell culture system using primary human tendon fibroblasts (tenocytes) and an established human mast cell line (HMC-1) 7 to identify adhesion receptors that mediate binding of human mast cells to tenocytes and their ECM proteins. Mast cells avidly bound to tenocytes upon co-culture, and the adhesion of mast cells to tissue culture plates in response to culture with tenocytederived conditioned media was primarily mediated by a5b1 integrin. Upon binding, mast cells acquired an elongated morphology and enhanced their expression of the adhesion receptor, THY1 (cluster of differentiation 90/CD90), events that were significantly dependent on a5b1 and fibronectin mediated interactions. Our data provides a mechanism by which human mast cells adhere to human tendon fibroblasts and its ECM components. MATERIAL AND METHODS Cell CultureTendon fibroblasts (tenocytes) were isolated from human hamstring tendon. Ethics approval was obtained from the review board at the University of British Columbia.3 Samples of healthy hamstring tendons from living male and female patients undergoing anterior cruciate ligament reconstruction were trimmed to remove fat and muscl...

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Section: Discussioncontrasting

confidence: 61%

Mechanism of mast cell adhesion to human tenocytes in vitro

Behzad

Tsai

Nassab

et al. 2014

Journal Orthopaedic Research

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“…Similar morphological alterations have been reported for mast cells. 40 SCF has been involved in migration and homing of hematopoietic stem cells, 41 melanoblasts, and primordial germ cells. 17 Induction of motility and migration can be correlated with enhancement of invasion and metastasis.…”

Section: Discussionmentioning

confidence: 99%

The Metastatic Ability of Ewing's Sarcoma Cells Is Modulated by Stem Cell Factor and by Its Receptor c-kit

Landuzzi

Giovanni

Nicoletti

et al. 2000

The American Journal of Pathology

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Ewing's sarcoma is a primitive highly malignant tumor of bone and soft tissues usually metastasizing to bone, bone marrow, and lung. Growth factor receptors and their ligands may be involved in its growth and dissemination. We analyzed the expression of c-kit and its ligand stem cell factor (SCF) in a panel of six Ewing's sarcoma cell lines. All cell lines exhibited substantial levels of surface c-kit expression, and five of six displayed transmembrane SCF on the cell surface. Expression of c-kit was down-modulated in all lines by exposure to exogenous SCF. The SCF treatment was able to confer to cells a growth advantage in vitro, due both to an increase in cell proliferation and to a reduction in the apoptotic rate. When used in the lower compartment of a migration chamber, SCF acted as a strong chemoattractant for Ewing's sarcoma cells. The pretreatment of cells with SCF reduced their chemotactic response to SCF. In athymic nude mice, Ewing's sarcoma cells injected intravenously metastasized to the lung and to a variety of extrapulmonary sites, including bone and bone marrow. Metastatic sites resembled those observed in Ewing's sarcoma patients and corresponded to SCF-rich microenvironments. The in vitro pretreatment of cells with SCF strongly reduced the metastatic ability of Ewing's sarcoma cells, both to the lung and to extrapulmonary sites. This could be dependent on the down-modulation of c-kit expression observed in SCF-pretreated cells, leading to a reduced sensitivity to the chemotactic and proliferative actions of SCF. Our results indicate that the response to SCF mediated by c-kit may be involved in growth, migration, and metastatic ability of Ewing's sarcoma cells.

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“…As depicted in figure 2, the concentrations of SCF in PF, as opposed to HGF, tended to decrease with the progression of endometriosis. SCF is known to stimulate mast cell functions [18][19][20]. Given that human endometrium has SCF receptors [21], it can be speculated that SCF in PF may affect the fertility of women with early stages of endometriosis by modulating functions of mast cells and/or endometrial cells.…”

Section: Peritoneal Environment In Endometriosisassociated Infertilitymentioning

confidence: 99%

Role of Laparoscopy in the Treatment of Endometriosis-Associated Infertility

Osuga

Koga²,

Tsutsumi³

et al. 2002

Gynecol Obstet Invest

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Endometriosis is assumed to be one of the causative factors of infertility, although the mechanism remains to be elucidated. Mechanical factors distorting the pelvic anatomy and/or changes in the biochemical and cellular environment in the peritoneal cavity are possible contributing factors impairing fertility. Laparoscopy is a widely used diagnostic and therapeutic means of treating endometriosis-associated infertility. However, pregnancy outcome after laparoscopy varies depending on preexisting tubal adhesions and endometriosis. Without tubal adhesions, the pregnancy rate is essentially the same irrespective of the presence or stages of endoemetriosis. Minimal/mild endometriosis benefited the most from laparoscopic manipulation when tubal adhesions were present. On the other hand, IVF-ET outcome of patients who previously underwent laparoscopy was not influenced by preexisiting tubal adhesions and endometriosis.

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Morphological alterations in rat peritoneal mast cells by stem cell factor

Cited by 6 publications

References 35 publications

Mechanism of mast cell adhesion to human tenocytes in vitro

Mechanism of mast cell adhesion to human tenocytes in vitro

The Metastatic Ability of Ewing's Sarcoma Cells Is Modulated by Stem Cell Factor and by Its Receptor c-kit

Role of Laparoscopy in the Treatment of Endometriosis-Associated Infertility

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