Morphological alterations induced by the exposure to TiO2 nanoparticles in primary cortical neuron cultures and in the brain of rats

Valentini, Xavier; Deneufbourg, Pauline; Paci, Paula; Rugira, Pascaline; Laurent, Sophie; Frau, Annica; Stanicki, Dimitri; Ris, Laurence; Nonclercq, Denis

doi:10.1016/j.toxrep.2018.08.006

Cited by 42 publications

(33 citation statements)

References 46 publications

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“…Animal were euthanized after different time intervals (4 days, 1 and 2 months) in order to observe acute and chronic toxicological effects of TiO 2 . The range of doses used in our study was in accordance with other toxicological and pharmacokinetic studies [22, 31–34]. Morphological and physiological damage induced by a single i.p.…”

Section: Discussionsupporting

confidence: 66%

Hepatic and Renal Toxicity Induced by TiO₂ Nanoparticles in Rats: A Morphological and Metabonomic Study

Valentini

Rugira

Frau

et al. 2019

Journal of Toxicology

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Titanium dioxide (TiO2) nanoparticles (NPs) are produced abundantly and are frequently used as a white pigment in the manufacture of paints, foods, paper, and toothpaste. Despite the wide ranges of uses, there is a lack of information on the impact of NPs on animal and human health. In the present study, rats were exposed to different doses of TiO2 nanoparticles and sacrificed, respectively, 4 days, 1 month, and 2 months after treatment. Dosage of TiO2 in tissues was performed by ICP-AES and revealed an important accumulation of TiO2 in the liver. The nanoparticles induced morphological and physiological alterations in liver and kidney. In the liver, these alterations mainly affect the hepatocytes located around the centrilobular veins. These cells were the site of an oxidative stress evidenced by immunocytochemical detection of 4-hydroxynonenal (4-HNE). Kupffer cells are also the site of an important oxidative stress following the massive internalization of TiO2 nanoparticles. Enzymatic markers of liver and kidney functions (such as AST and uric acid) are also disrupted only in animals exposed to highest doses. The metabonomic approach allowed us to detect modifications in urine samples already detectable after 4 days in animals treated at the lowest dose. This metabonomic pattern testifies an oxidative stress as well as renal and hepatic alterations.

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Section: Discussionsupporting

confidence: 66%

Hepatic and Renal Toxicity Induced by TiO₂ Nanoparticles in Rats: A Morphological and Metabonomic Study

Valentini

Rugira

Frau

et al. 2019

Journal of Toxicology

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“…The influence of TiO 2 on the central nervous system (CNS) is gaining attention recently. Valentini et al investigated the toxicity of TiO 2 -NP to the cortical neuron cultures and in the brain of rats, reported the clear impact of TiO 2 -NP on the neuronal cells and rat brain, and indicated the new evidences of TiO 2 -NP toxicity in CNS [32].…”

Section: Resultsmentioning

confidence: 99%

“…Animal experiments, especially in rodents, are being conducted to study the effects of titanium oxide nanoparticles on the human body. In these experiments, titanium was overdosed in rats and the distribution of titanium in the liver, spleen, bone marrow, lungs, brain, and kidneys and titanium-related problems were found in each tissue [32, 33, 90]. In particular, lung problems, cytotoxic reactions, inflammation reactions, fibrosis, and tumors were observed.…”

Section: Discussionmentioning

confidence: 99%

General review of titanium toxicity

et al. 2019

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Background Titanium is a commonly used inert bio-implant material within the medical and dental fields. Although the use of titanium is thought to be safe with a high success rate, in some cases, there are rare reports of problems caused by titanium. In most of these problematic reports, only individual reports are dominant and comprehensive reporting has not been performed. This comprehensive article has been prepared to review the toxicity of titanium materials within the medical and dental fields. Methods We used online searching tools including MEDLINE (PubMed), Embase, Cochrane Library, and Google Scholar by combining keywords such as “titanium implant toxicity,” “titanium implant corrosion,” “titanium implant allergy,” and “yellow nail syndrome.” Recently updated data has been collected and compiled into one of four categories: “the toxicity of titanium,” “the toxicity of titanium alloys,” “the toxicity of titanium implants,” and “diseases related to titanium.” Results Recent studies with regard to titanium toxicity have been increasing and have now expanded to the medical field in addition to the fields of environmental research and basic science. Problems that may arise in titanium-based dental implants include the generation of titanium and titanium alloy particles and ions deposited into surrounding tissues due to the corrosion and wear of implants, resulting in bone loss due to inflammatory reactions, which may lead to osseointegration failure of the dental implant. These titanium ions and particles are systemically deposited and can lead to toxic reactions in other tissues such as yellow nail syndrome. Additionally, implant failure and allergic reactions can occur due to hypersensitivity reactions. Zirconia implants can be considered as an alternative; however, limitations still exist due to a lack of long-term clinical data. Conclusions Clinicians should pay attention to the use of titanium dental implants and need to be aware of the problems that may arise from the use of titanium implants and should be able to diagnose them, in spite of very rare occurrence. Within the limitation of this study, it was suggested that we should be aware the rare problems of titanium toxicity.

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“…Valentini et al observed a characteristic phenotype of activated microglia cells caused by the presence of TiO 2 NPs. Their morphological changes, such as the larger size and formation of membrane protrusions typical of phagocytosis, indicated the uptake of TiO 2 NPs through this process [73].…”

Section: Cellular Uptakementioning

confidence: 99%

Toxicity of metallic nanoparticles in the central nervous system

Sawicki

Czajka

Matysiak‐Kucharek

et al. 2019

Nanotechnology Reviews

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Metallic nanoparticles due to their small size and unique physico-chemical characteristics have found excellent applications in various branches of industry and medicine. Therefore, for many years a growing interest has been observed among the scientific community in the improvement of our understanding of the impact of nanoparticles on the living organisms, especially on humans. Considering the delicate structure of the central nervous systemit is one of the organs most vulnerable to the adverse effects of metallic nanoparticles. For that reason, it is important to identify the modes of exposure and understand the mechanisms of the effect of nanoparticles on neuronal tissue. In this review, an attempt is undertaken to present current knowledge about metallic nanoparticles neurotoxicity based on the selected scientific publications. The route of entry of nanoparticles is described, as well as their distribution, penetration through the cell membrane and the blood-brain barrier. In addition, a study on the neurotoxicity in vitro and in vivo is presented, as well as some of the mechanisms that may be responsible for the negative effects of metallic nanoparticles on the central nervous system. Graphical abstract: This review summarizes the current knowledge on the toxicity of metallic NPs in the brain and central nervous system of the higher vertebrates.

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Morphological alterations induced by the exposure to TiO2 nanoparticles in primary cortical neuron cultures and in the brain of rats

Abstract: Graphical abstract

Cited by 42 publications

References 46 publications

Hepatic and Renal Toxicity Induced by TiO₂ Nanoparticles in Rats: A Morphological and Metabonomic Study

Hepatic and Renal Toxicity Induced by TiO₂ Nanoparticles in Rats: A Morphological and Metabonomic Study

General review of titanium toxicity

Toxicity of metallic nanoparticles in the central nervous system

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Morphological alterations induced by the exposure to TiO2 nanoparticles in primary cortical neuron cultures and in the brain of rats

Abstract: Graphical abstract

Cited by 42 publications

References 46 publications

Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study

Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study

General review of titanium toxicity

Toxicity of metallic nanoparticles in the central nervous system

Contact Info

Product

Resources

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Hepatic and Renal Toxicity Induced by TiO₂ Nanoparticles in Rats: A Morphological and Metabonomic Study

Hepatic and Renal Toxicity Induced by TiO₂ Nanoparticles in Rats: A Morphological and Metabonomic Study