Morphological analysis of leucocyte transmigration in the pleural cavity

Teng, Ruifeng; Johkura, Kohei; Ogiwara, Naoko; Zhao, X; Cui, Li; Iida, I; Okouchi, Yasumitsu; Asanuma, Kazuhiko; Sasaki, Katsunori

doi:10.1046/j.1469-7580.2003.00231.x

Cited by 11 publications

(18 citation statements)

References 38 publications

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“…Osmium plasma coating has one great advantage for high resolution observations using SEM because it gives sufficient conductivity for thinner films compared to the conventional gold-sputtered film. [10] The top part of Figure 3 contains low magnification images, showing that all the samples exhibited diverse surface features. PS exhibited regularly spaced parallel bands perpendicular to the crack direction (Figure 3(a)).…”

Section: Resultsmentioning

confidence: 99%

Study of Adhesion and Fracture of Polymer Laminates by Imaging of Interfaces

Horiuchi

Yin

Liao

et al. 2007

Macromol. Rapid Commun.

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The adhesion and fracture of styrene‐acrylonitrile random copolymer and poly(methyl methacrylate) (PMMA/SAN) laminates were studied. They showed a drastic transition from brittle to ductile on varying the acrylonitrile (AN) content in SAN, with changes in the fracture mode from interfacial failure to cohesive fracture. Energy‐filtering transmission electron microscopy (EFTEM) and scanning electron microscopy (SEM) with an in‐lens detector system were employed to study the interface and adhesion of the laminates. The effect of the AN content in SAN on the PMMA/SAN interfacial structures could be revealed by imaging of the interfaces using elemental mapping and electron energy loss spectroscopy (EELS). The in‐lens detector system in the SEM enabled the differentiation of thin interfaces with poor adhesion strength, yielding smooth and flat fracture surfaces, where numerous nanosized fibrils were formed normal to the surfaces.magnified image

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Section: Resultsmentioning

confidence: 99%

Study of Adhesion and Fracture of Polymer Laminates by Imaging of Interfaces

Horiuchi

Yin

Liao

et al. 2007

Macromol. Rapid Commun.

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“…Although investigators have observed changes in integrin expression after transendothelial migration [10,11], little has been done to investigate the functionality of these integrins after transendothelial migration. In this study, we provide evidence that integrin-mediated motility is altered following neutrophil transendothelial migration.…”

Section: Introductionmentioning

confidence: 99%

Transendothelial migration enhances integrin-dependent human neutrophil chemokinesis

Gonzalez

Elbjeirami

West

et al. 2006

Journal of Leukocyte Biology

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Transendothelial migration of neutrophils induces phenotypic changes that influence the interactions of neutrophils with extravascular tissue components. To assess the influence of transmigration on neutrophil chemokinetic motility, we used polyethylene glycol hydrogels covalently modified with specific peptide sequences relevant to extracellular matrix proteins. We evaluated fMLP-stimulated human neutrophil motility on peptides Arg-Gly-Asp-Ser (RGDS) and TMKIIPFN-RTLIGG (P2), alone and in combination. RGDS is a bioactive sequence found in a number of proteins, and P2 is a membrane-activated complex-1 (Mac-1) ligand located in the ␥-chain of the fibrinogen protein. We evaluated, via video microscopy, cell motility by measuring cell displacement from origin and total accumulated distance traveled and then calculated average velocity. Results indicate that although adhesion and shape change were supported by hydrogels containing RGD alone, motility was not. Mac-1-dependent motility was supported on hydrogels containing P2 alone. Motility was enhanced through combined presentation of RGD and P2, engaging Mac-1, ␣ V ␤ 3 , and ␤ 1 integrins. Naïve neutrophil motility on combined peptide substrates was dependent on Mac-1, and ␣ 4 ␤ 1 while ␣ 6 ␤ 1 contributed to speed and linear movement. Transmigrated neutrophil motility was dependent on ␣ v ␤ 3 and ␣ 5 ␤ 1 , and ␣ 4 ␤ 1 , ␣ 6 ␤ 1 , and Mac-1 contributed to speed and linear motion. Together, the data demonstrate that efficient neutrophil migration, dependent on multi-integrin interaction, is enhanced after transendothelial migration. J. Leukoc. Biol. 81: 686 -695; 2007.

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“…A pivotal role for the β1 and β2-integrins, interacting with their epithelial ligands ICAM-1 and VCAM, has been described [10,11]. ICAM-1 expression is upregulated in different kidney disorders associated with inflammation such as acute transplant rejection, rapid progressive glomerulonephritis or acute renal failure due to ischemia and/or reperfusion [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Neutrophil Transmigration Across Renal Proximal Tubular HK-2 Cells

Bijuklic

Sturn

Jennings

et al. 2006

Cell Physiol Biochem

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Background: Adhesion of intratubular leukocytes to proximal tubules in biopsies of patients with rapidly progressive glomerulonephritis and the appearance of leukocytes in the urine in interstitial nephritis suggest interactions between leukocytes and tubular epithelia in renal diseases. The aim of this study was to investigate the effect of cytokines and endotoxin on leukocyte migration through proximal tubular epithelial cells and also to determine the role of the transmembrane adhesion molecules ICAM-1 and CD47 in this process. Methods: Experiments determined transepithelial migration (TEM) of PMN (polymorphonuclear) leukocytes through monolayers of HK-2. Expression of ICAM-1 and CD47 was assessed via confocal immunofluorescence, FACS analysis and western blotting. The effect of antibodies against ICAM-1 and CD47 on TEM was examined. Furthermore measurements of cytokine release (IL- 6 and IL-8) were performed. Results: Preincubation of HK-2 cells with either TNFα or LPS resulted in stimulation of PMN migration through monolayers of HK-2 cells. There was no preferred direction of transmigration. ICAM-1 was expressed by HK-2 cells and expression was increased after 4 h stimulation with TNFα or LPS. Application of ICAM-1 antibodies inhibited TEM. CD47 was expressed in both HK-2 cells and PMN. CD47 antibodies inhibited predominantly basolateral-to-apical TEM. HK-2 cells released IL-8 and IL-6 preferably into the apical compartment. Additionally, we showed that fMLP induced transmigration through monolayers of HK-2 cells was associated with significant increased CD47 expression on PMN cell surfaces. Conclusions: Inflammatory mediators stimulate TEM of PMN through monolayers of HK-2 cells without a clearly discernible preference of direction. Mechanisms involved in TEM stimulated by cytokines or endotoxin appear to be mainly changes in surface receptor densities of HK-2 cells with ICAM-1 and CD47 playing an essential role.

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Morphological analysis of leucocyte transmigration in the pleural cavity

Cited by 11 publications

References 38 publications

Study of Adhesion and Fracture of Polymer Laminates by Imaging of Interfaces

Study of Adhesion and Fracture of Polymer Laminates by Imaging of Interfaces

Transendothelial migration enhances integrin-dependent human neutrophil chemokinesis

Mechanisms of Neutrophil Transmigration Across Renal Proximal Tubular HK-2 Cells

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