Morphological and behavioural development of halibut, <i>Hippoglossus hippoglossus</i> (L.) larvae

Pittman, Karin; Skiftesvik, Anne Berit; Berg, Leif

doi:10.1111/j.1095-8649.1990.tb05876.x

Cited by 115 publications

(93 citation statements)

References 9 publications

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“…The fertilisation rate was approximately 95 %. Before transfer to the laboratory at 60.5 degree-days, the eggs were reared at 6°C in a 250 1 upwelling egg incubator for 10 d (Pittman et al 1990). Nodavirus was not detected in the gonadal fluids of the spawners employing RT-PCR according to Nishizawa et al (1994).…”

Section: Methodsmentioning

confidence: 99%

Surface disinfection of Atlantic halibut Hippoglossus hippoglossus eggs with ozonated sea-water inactivates nodavirus and increases survival of the larvae

Grotmol¹,

Totland²

2000

Dis. Aquat. Org.

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Disinfection by ozonation of sea-water may reduce the risk of transmission of nodavirus, a major fish pathogen, via Atlantic halibut Hippoglossus hippoglossus eggs. In the present study, eggs at 4 d prior to hatching were exposed to nodavirus and then to ozonated sea-water using different concentrations (0.3 to 10 mg 1-') and exposure times (0.5 to 10 min). None of the larvae from virus-exposed eggs washed with ozonated sea-water developed viral encephalopathy and retinopathy (VER), which was detected in all dead larvae from eggs exposed to nodavirus but not washed with ozonated seawater. In the non-treated control group about 20% of the dead larvae developed the disease. This suggests that the halibut eggs taken from a large-scale production facility were already contaminated with nodavirus. The egg groups which had been treated with 4 mg O3 1-' for 0.5 rnin or with lower total ozone exposures had a higher survival and no adverse effects on the development of the larvae after hatching were observed. Although a slight delay in hatching was found, after 2 d the cumulative hatching had normalised. In the egg groups with high total exposure (4 mg O3 1-' for 1 min or higher total ozone exposures) a pronounced negative effect on hatching was observed. Our results indlcate that the egg surface may be important in the transfer of nodavirus and that nodavirus associated with the surface of the egg may be inactivated by ozonated sea-water.

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Section: Methodsmentioning

confidence: 99%

Surface disinfection of Atlantic halibut Hippoglossus hippoglossus eggs with ozonated sea-water inactivates nodavirus and increases survival of the larvae

Grotmol¹,

Totland²

2000

Dis. Aquat. Org.

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“…Samples of larvae were collected at five developmental periods: hatching (S1), mouth opening (S2), postnotochord flexion/pro-metamorphosis (S6), active metamorphosis (S7-S9), and late metamorphosis/juvenile (S9C) ( Table 2). The size and morphology of the larvae in these collections were compared with published staging descriptions for Atlantic halibut (Pittman et al 1990, Saele et al 2004. Additional samples of larvae from prometamorphic to early climax metamorphosis (S7-S9) were taken and graded according to size and developmental stage (Table 3).…”

Section: Sampling Of Larval Halibutmentioning

confidence: 99%

“…Many organs are in a rudimentary state and develop functionality during the larval period. For instance, the mouth is not functional and a differentiated stomach is absent at hatching (Pittman et al 1990, Murray et al 2006. Major developmental stages during larval growth include the onset of exogenous feeding (first feeding) and metamorphosis from the larval to juvenile stage.…”

Section: Introductionmentioning

confidence: 99%

Ontogenetic and tissue-specific expression of preproghrelin in the Atlantic halibut, Hippoglossus hippoglossus L.

Manning¹,

Murray²,

Gallant³

et al. 2007

Journal of Endocrinology

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Ghrelin is a conserved vertebrate hormone that affects both GH release and appetite. We have cloned and characterized Atlantic halibut preproghrelin cDNA and examined for the first time preproghrelin expression during fish larval development using quantitative real-time PCR. In addition, cellular sites of expression in larvae and tissue-specific expression in 3-year-old halibut were studied. A full-length cDNA for preproghrelin was isolated from halibut stomach tissue. The 899 bp cDNA encodes an open reading frame of 105 amino acids that is comprised of a signal peptide and two peptides with high similarity to ghrelin and obestatin. The deduced amino acid sequence of halibut ghrelin peptide (GSSFLSPSHKPPKGKPPRA) shows significant conservation relative to other teleostean sequences and is identical to human ghrelin for the first seven amino acids of the sequence. The putative obestatin peptide is well-conserved among fishes but shares limited similarity with its human counterpart. Expression of ghrelin was localized to two different cell types in the stomach of larval halibut by in situ hybridization. However, sensitive PCR assays on tissues collected from 3-year-old fish additionally identified ghrelin transcripts in pyloric caecae, intestine, and in immature ovary and testis. Ontogenetic studies detected ghrelin expression prior to exogenous feeding during larval development (hatching and mouth-opening stages) with increased expression occurring through metamorphosis. This increase was pronounced during climax metamorphosis and coincided with stomach differentiation. Patterns of preproghrelin expression suggest that ghrelin has important roles during and after larval development in halibut, and that ghrelin is associated with digestive and gonadal tissues in this teleost.

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“…Development of the jaw appears to be a labile phase in larval development that is subject to interference from both environmental and biological factors, such that jaw malformations are common, but manifest in different forms (Hickey et al 1977;Barahona-Fernandes 1982;Pittman et al 1990a;Morrison & MacDonald 1995;Cobcroft et al 2001). Jaw malformations may have lethal or sublethal effects (e.g., poor growth), through the inability of fish to feed adequately (Barahona-Fernandes 1982;Pittman etal.…”

Section: Introductionmentioning

confidence: 99%

Jaw malformation in cultured yellowtail kingfish(Seriola lalandi)larvae

Cobcroft¹,

Pankhurst

Poortenaar

et al. 2004

New Zealand Journal of Marine and Freshwater Research

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Several jaw malformations are common in cultured finfish larvae. Hatchery-reared yellowtail kingfish (Seriola lalandi) larvae were cleared and stained to examine cartilage and bone structure of the jaw. One malformation, characterised by a lowered hyoid arch, was present in kingfish larvae on days 4 and 8 post-hatching, but not on day 12. A different malformation was present in larvae on day 16, typified by abnormal positioning of the lower jaw and hyoid arch, and breakage of the Meckel' s cartilage. Evidence of jaw malformations as early as day 4 suggests that broodstock nutrition may be a factor in the jaw malformation of kingfish larvae, but other potential causes are environmental factors in culture and larval nutrition.

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Morphological and behavioural development of halibut, Hippoglossus hippoglossus (L.) larvae

Cited by 115 publications

References 9 publications

Surface disinfection of Atlantic halibut Hippoglossus hippoglossus eggs with ozonated sea-water inactivates nodavirus and increases survival of the larvae

Surface disinfection of Atlantic halibut Hippoglossus hippoglossus eggs with ozonated sea-water inactivates nodavirus and increases survival of the larvae

Ontogenetic and tissue-specific expression of preproghrelin in the Atlantic halibut, Hippoglossus hippoglossus L.

Jaw malformation in cultured yellowtail kingfish(Seriola lalandi)larvae

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