Microorganisms have a substantial impact on the environment and have been observed to either immunize or infect organisms from all kingdoms. Plants and microorganisms have undergone co-evolution and engage in interactions within their natural environment. Plant microbiota, often known as plant-associated microorganisms, are essential components of plant existence. Similar to other living systems, the plant body interacts with a range of living and non-living factors as it develops in an intricate environment. The plant microbiome can sustain disease suppression by providing essential tasks to their host, such as stress tolerance, vitality, and development. Plants and microorganisms have evolved molecular systems to mutually interact and derive advantages from their interaction. This symbiotic relationship is advantageous for both the microorganisms and the plants. Various pathways have been explored and are currently being utilized to achieve agricultural, environmental, and health benefits, based on this molecular interaction. Plant-associated microorganisms serve a vital role in the growth and development of plants. Microorganisms stimulate plant growth by directly increasing nutrient uptake and stimulating hormone production. The potential benefits of these mutually beneficial interactions between plants and microbes could be connected to develop microbial inoculants for application in agricultural biotechnology. In general, the utilization of microorganisms and the establishment of beneficial plant-microbe interactions offer promising and environmentally favourable approaches for sustainable and organic agriculture worldwide.