Morphological and biochemical characterization of isolated Aspergillus niger, Saccharomyces cerevisiae and Zymomonas mobilis from local indigenous sources

Tambuwal, A D; Muhammad, Inusa Babanladi; Sani, Alhaji; Sirajo, Muhammad; Ogbiko, Cyril

doi:10.30574/gscbps.2018.5.3.0147

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…The study shows that Zymomonas mobilis is seen to have brilliant white to cream colour, plumb white with round ends, Gram negative, motile, catalase positive, oxidase and urease negative, produces gas from glucose, fructose, and sucrose. These findings are in agreement with that of reference Obire [20] who reported the isolation of Zymomonas mobilis from fresh palm wine saps and Tambuwal et al [29] also reported the isolation Zymomonas mobilis from rotten oranges.…”

Section: Cs= Citrus Sinensissupporting

confidence: 93%

“…Saccharomyces cerevisiae are seen to be smooth creamish, white spherical shape, Grampositive, non-motile, catalase positive, the organisms was able to ferment glucose, fructose, sucrose, maltose and galactose, producing acid and gas. The result is in agreement with that of Rabah et al [19] who isolated Saccharomyces cerevisiae from palm wine [17] who isolated Saccharomyces cerevisiae from palm wine juice in sake-type fermentation, Moneke et al [30] reported the isolation of Saccharomyces cerevisiae from orchard soil and Tambuwal et al [29] reported the isolation of Saccharomyces cerevisiae from Hibiscus sadriffa (Zobo).…”

Section: Saccharomyces Cerevisiae Isolated From Locally Fermeneted Beverage ("Kunun-zaki") Samplessupporting

confidence: 90%

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Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Na’Allah

Iliyasu

Haruna

et al. 2021

MRJI

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Background of Study: Plant waste such as rice husk and groundnut shell are generated in large amounts, these waste presents a tremendous pollution to the environment. Worldwide, these wastes are often simply dumped into landfills and oceans or used as animal feeds. The recovery of food processing wastes as renewable energy sources represents a sustainable option for the substitution of fossil energy in order to minimize environmental damages and to meet energy demands of the growing population. Aim: To produce bioethanol from rice husk and groundnut shell using local strains of Zymomonas mobilis and Saccharomyces cerevisiae. Place and Duration of Study: Conducted at the Microbiology Laboratory of Abubakar Tafawa Balewa University Bauchi, Bauchi state, Nigeria, between April to June, 2021. Methods: Groundnut shell and Rice husk were collected from local milling center. The wastes were powdered, sieved and used as carbon source. Proximate composition of the subsrate was done and the total carbohydrate was determined by difference. The sum of the percentage moisture, ash, crude lipid, crude protein and crude fibre was subtracted from 100. Zymomonas mobilis and Saccharomyces cerevisiae were isolated from rotten sweet oranges and locally fermented beverage (‘kunun-zaki’) respectively by growing them on Malt Yeast Peptone Glucose Agar (MYPGA) after which they were further screened for their ability to tolerate ethanol and they serve as organisms for fermentation. The enzyme α- amylase was used for hydrolysis. The fermented substrates were distilled at 78oC and the distillate was collected as bioethanol in a conical flask. UV-VIS spectrophotometer was used to determine the absorbance of each concentration (0, 0.2, 0.4, 0.6 and 0.8cm3) of reducing sugar content of the hydrolysates and the bioethanol produced by developing a standard curve at a wavelength of 491nm and 588nm respectively. The concentration of reducing sugar and bioethanol was determined using a reference line from the Standard curve. Results: Proximate analysis done shows that rice husk have 70.09% carbohydrates while groundnut shell has 65.09% carbohydrates. Groundnut shell yielded the highest reducing sugar of 5.096%. Rice husk yielded the lowest quantity of reducing sugar with a total yield of 2.962%. Maximum concentration of bioethanol of 0.971% was produced from the combination of Saccharomyces cerevisiae and Zymomonas mobilis from groundnut shell. The lowest concentration of 0.121% of bioethanol was produced when Saccharomyces cerevisiae was used on rice husk hydrolysates. The synergistic relationship of Saccharomyces cerevisiae and Zymomonas mobilis yielded the maximum bioethanol when compared with the yield obtained when the organisms were used singly. Zymomonas mobilis produced highest bioethanol content when the organisms are used single. Conclusion: This study demonstrates the potentiality of local strains of Saccharomyces cerevisiae and Zymomonas mobilis isolated from rotten sweet orange and locally fermented beverage (‘kunun-zaki’) to produce bioethanol by fermenting the rice husk and groundnut shell hydrolysates.

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Section: Cs= Citrus Sinensissupporting

confidence: 93%

Section: Saccharomyces Cerevisiae Isolated From Locally Fermeneted Beverage ("Kunun-zaki") Samplessupporting

confidence: 90%

Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Na’Allah

Iliyasu

Haruna

et al. 2021

MRJI

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“…After 24 h of growth, colony shape, motility, features, surface, and pigmentation were studied. Method of biochemical characterizations by Tambuwal et al (2018) was used.…”

Section: Bacteriologymentioning

confidence: 99%

Biodegradation and Bioremediation of Polymer by Microbial Assisted Novel Process

A.K.¹,

Soundarya²,

Sirajudeen³

2022

IJZI

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The degradation of polystyrene (PS) and polyurethane (PU), both are foams, is a budding challenge due to increasing white pollution. The present investigation has focused on the microbial assisted biodegradation. Various indigenous microorganisms were isolated from sewage soil. PS – PU foam and bioplastic was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay such as soil burial and liquid culture in the presence of PS – PU foams and bioplastic in a growth medium. Two microorganism were isolated-- Pseudomonas aeruginosa and Aspergillus niger. The degradation rate based on weight loss is conducted by in vitro assay for 10 and 35 days. The maximum degraded foam was analyzed through Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy and PS – PU foam and bioplastic act as a control without microorganisms. Results of the present study showed that the maximum degradation and weight loss in the polyurethane foam occurred in the soil burial method by Pseudomonas aeruginosa compared with Aspergillus niger. The soil burial method is more significant than liquid culture method. There is no weight reduction in the polystyrene foam in both organism and methods. For the degradation of synthetic polymers the isolated Pseudomonas aeruginosa and Aspergillus niger were encapsulated for future studies.

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Bioethanol production from leftover food by yeasts isolated from fruit at Ambo University, Ethiopia

Sisay,

keneni,

Kumsa

2023

Environ Dev Sustain

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Morphological and biochemical characterization of isolated Aspergillus niger, Saccharomyces cerevisiae and Zymomonas mobilis from local indigenous sources

Cited by 3 publications

References 17 publications

Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Biodegradation and Bioremediation of Polymer by Microbial Assisted Novel Process

Bioethanol production from leftover food by yeasts isolated from fruit at Ambo University, Ethiopia

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