Morphological and biomolecular characterization of the neonatal olfactory bulb ensheathing cell line

Audisio, Chiara; Raimondo, Stefania; Nicolino, S.; Gambarotta, Giovanna; Scipio, Federica Di; Macrì, Loredana; Montarolo, Francesca; Giacobini-Robecchi, Maria G.; Porporato, Paolo E.; Filigheddu, Nicoletta; Graziani, Andrea; Geuna, Stefano; Perroteau, Isabelle

doi:10.1016/j.jneumeth.2009.09.021

Cited by 19 publications

(18 citation statements)

References 35 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NOBEC are characterized by high migratory activity [27], which could be due to an autocrine stimulation mediated by endogenous soluble NRG1 on endogenous ErbB3-ErbB2 heterodimer.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Glial Cell Models andIn VitroManipulation of the Neuregulin1/ErbB System

Pascal

Giovannelli

Gnavi

et al. 2014

BioMed Research International

Self Cite

View full text Add to dashboard Cite

The neuregulin1/ErbB system plays an important role in Schwann cell behavior both in normal and pathological conditions. Upon investigation of the expression of the neuregulin1/ErbB system in vitro, we explored the possibility to manipulate the system in order to increase the migration of Schwann cells, that play a fundamental role in the peripheral nerve regeneration. Comparison of primary cells and stable cell lines shows that both primary olfactory bulb ensheathing cells and a corresponding cell line express ErbB1-ErbB2 and neuregulin1, and that both primary Schwann cells and a corresponding cell line express ErbB2-ErbB3, while only primary Schwann cells express neuregulin1. To interfere with the neuregulin1/ErbB system, the soluble extracellular domain of the neuregulin1 receptor ErbB4 (ecto-ErbB4) was expressed in vitro in the neuregulin1 expressing cell line, and an unexpected increase in cell motility was observed. In vitro experiments suggest that the back signaling mediated by the transmembrane neuregulin1 plays a role in the migratory activity induced by ecto-ErbB4. These results indicate that ecto-ErbB4 could be used in vivo as a tool to manipulate the neuregulin1/ErbB system.

show abstract

“…NOBEC are characterized by high migratory activity [27], which could be due to an autocrine stimulation mediated by endogenous soluble NRG1 on endogenous ErbB3-ErbB2 heterodimer.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Glial Cell Models andIn VitroManipulation of the Neuregulin1/ErbB System

Pascal

Giovannelli

Gnavi

et al. 2014

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…After 5 days of induction, cells were fixed with 4% paraformaldehyde for 20 min, washed with PBS, incubated with Nile Red (SigmaAldrich) for 10 min to emphasize lipid vesicles occurrence [32] and then observed by confocal laser microscope. For electron microscopy, differentiated cells were processed as previously described [29].…”

Section: Differentiation Culture Conditionsmentioning

confidence: 99%

Self-Renewal and Multipotency Coexist in a Long-Term Cultured Adult Rat Dental Pulp Stem Cell Line: An Exception to the Rule?

Sprio

Scipio

Raimondo

et al. 2012

Stem Cells and Development

Self Cite

View full text Add to dashboard Cite

SummaryThe stemness state is characterized by self-renewal and differentiation properties.However, stem cells are not able to preserve these characteristics in long-term culture because of the intrinsic fragility of their phenotype easily undergoing senescence or neoplastic transformation. Furthermore, although isolated from the same original tissue using similar protocols, adult stem cells can display dissimilar phenotypes and important cell clone/species contamination. Finally, the lack of a clear standardization contributes to complicate the comprehension about the stemness condition. In this context, cell lines displaying a particularly stable phenotype must be identified in order to define one or multiple benchmarks against which other stem cell lines could be reliably assessed. The present paper demonstrates that it is possible to isolate from the rat dental pulp a stem cell

show abstract

“…A dissolution test was carried out in order to study the factors influencing the fibre dissolution rate, such as glass composition, fibre diameter and thermal treatment. Finally, biological tests were carried out in or-der to investigate the interaction between fibres and either neona-tal olfactory bulb ensheathing cell (NOBEC), a type of glial cell [24], or dorsal root ganglia (DRG) dissociated neurons.…”

Section: Introductionmentioning

confidence: 99%

Phosphate glass fibres and their role in neuronal polarization and axonal growth direction

et al. 2012

View full text Add to dashboard Cite

Morphological and biomolecular characterization of the neonatal olfactory bulb ensheathing cell line

Cited by 19 publications

References 35 publications

Characterization of Glial Cell Models andIn VitroManipulation of the Neuregulin1/ErbB System

Characterization of Glial Cell Models andIn VitroManipulation of the Neuregulin1/ErbB System

Self-Renewal and Multipotency Coexist in a Long-Term Cultured Adult Rat Dental Pulp Stem Cell Line: An Exception to the Rule?

Phosphate glass fibres and their role in neuronal polarization and axonal growth direction

Contact Info

Product

Resources

About