Morphological and cytogenetic characterization of new ornamental Passiflora hybrids (P. ‘Vivis’ and P. ‘Jhovi’)

Souza, Viviane de Oliveira Nogueira; Souza, Margarete Magalhães; Silva, G. S.; Melo, Cláusio Antônio Ferreira de; Freitas, Jôsie Cloviane de Oliveira; Souza, Analu Cruz

doi:10.1590/1984-70332020v20n3a47

Cited by 4 publications

(9 citation statements)

References 22 publications

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“…Finally, the results of crosses between species of subclades 1 and 2 were mixed, indicating compatibility of seven combinations (P. galbana x P. alata; P. galbana x P. edulis; P. quadrangularis x P. alata; P. edulis x P. caerulea; P. mucronata x P. alata, P. setacea x P. alata; P. setacea x P. amethystine) and no viable seed set of three others (P. edulis x P. tenuifila, P. caerulea x P. edulis, P. amethystina x P. edulis, P. serratodigitata x P. alata). Elsewhere, successful crosses displaying normal meiotic behavior have been achieved between P. coccinea and P. hatschbachii (Souza et al 2020). Some crosses were only successful in one direction, as for example P. caerulea x P. edulis, for which compatibility was only observed in one direction of the cross (P. edulis x P. caerulea).…”

Section: Pw Inglis Et Almentioning

confidence: 99%

Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

Inglis

Bellon

Junqueira

et al. 2021

Crop Breed. Appl. Biotechnol.

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Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably.

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Section: Pw Inglis Et Almentioning

confidence: 99%

Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

Inglis

Bellon

Junqueira

et al. 2021

Crop Breed. Appl. Biotechnol.

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“…However, in Brazil, the use of these species for ornamental purpose is minimal, while in Europe these species are used for the decoration of pergolas, walls, and gardens (Junqueira et al 2008;Abreu et al 2009;Conceição et al 2011). In addition, arti cial Passi ora hybrids can be developed without many di culties (Vanderplank 2000) because of weak reproductive barriers (Belo et al 2018;Souza et al 2020). The morphology of the species, owers, and especially of the androgynophore which are composed of complex corona and consisting of laments arranged in one or more concentric rows, are beautiful.…”

Section: Introductionmentioning

confidence: 99%

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Souza

Silva

et al. 2022

Euphytica

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Interspeci c hybrids can be studied using methodologies in which the male gamete with high reproduction potential, viability, and fertility is prioritized. Passi ora species, with lush, showy, and exotic colors, have great potential for the ornamental plant market. In addition, arti cial Passi ora hybrids were developed without many di culties because of weak reproductive barriers. Thus, meiotic and post-meiotic behaviors were analyzed with 2% acetic carmine staining. Con rmation of interspeci c hybridization was performed using SSR markers and GISH technique was used to detect genomic differentiation. The pollen viability of the parental and hybrids genotypes was tested using Alexander solution, uorescein diacetate and in vitro germination tests were performed using culture medium. The meiotic behavior was regular and displayed haploid number n = 9 with nine bivalent (II) chromosomal, pairing in 90% of the cells in diakinesis. There was a signi cant difference (p < 0.05) in terminal and interstitial chiasma frequencies. Meiotic irregularities observed were as follows: early and/or delayed chromosomes, disorientation of spindle bers, transverse spindles, tripolar spindles, and asynchrony; and consequently irregular post-meiotic products were observed: monads, dyads, triads, and polyads. GISH was used in the interspeci c hybrids and pairing between homeologous chromosomes, and bivalent and tetravalent formation were observed. From this study, we could conclude that hybrid genotypes are fertile and pollen grains are viable and can be used in breeding programs. We also hypothesize that interspeci c hybrid genotypes of Passi ora can be obtained with regular meiosis, which could be viable and fertile.

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“…Regular meiotic behavior enables the formation of viable gametes (Belo et al 2018), and thus, the formation of fertile hybrids (Lavinscky et al 2017).Time and cost reduction are interesting factors for improvement programs. The costs of plant maintenance can be reduced by con rming the hybridization still in seedling phase, and molecular markers have been widely used for this purpose (Bellon et al 2007;Junqueira et al 2008;Conceição et al 2011), in addition to genomic in situ hybridization (GISH) techniques (Silva and Souza 2013;Souza et al 2020). Molecular markers are also used to verify the occurrence of DNA polymorphism (Fajardo et al 1998) once the markers generate information on allelic diversity of the analyzed groups and phylogenetic relationships .…”

mentioning

confidence: 99%

“…Studies have been already performed in which SSR were used to con rm interspeci c hybridization between species of the genus Passi ora (Melo et al 2016). In addition, successful assessment of the transferability rates of SSR primers in wild species of Passi ora has been made (Silva et al 2014).GISH can be used to con rm interspeci c crossings (Souza et al 2020); this technique can be explored by distinguishing the parental genomes in hybrid genotypes, differentiating the chromosomal lots from the supposed parents (…”

mentioning

confidence: 99%

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Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Souza

Silva

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Interspecific hybrids can be studied using methodologies in which the male gamete with high reproduction potential, viability, and fertility is prioritized. Passiflora species, with lush, showy, and exotic colors, have great potential for the ornamental plant market. In addition, artificial Passiflora hybrids were developed without many difficulties because of weak reproductive barriers. Thus, meiotic and post-meiotic behaviors were analyzed with 2% acetic carmine staining. Confirmation of interspecific hybridization was performed using SSR markers and GISH technique was used to detect genomic differentiation. The pollen viability of the parental and hybrids genotypes was tested using Alexander solution, fluorescein diacetate and in vitro germination tests were performed using culture medium. The meiotic behavior was regular and displayed haploid number n = 9 with nine bivalent (II) chromosomal, pairing in 90% of the cells in diakinesis. There was a significant difference (p < 0.05) in terminal and interstitial chiasma frequencies. Meiotic irregularities observed were as follows: early and/or delayed chromosomes, disorientation of spindle fibers, transverse spindles, tripolar spindles, and asynchrony; and consequently irregular post-meiotic products were observed: monads, dyads, triads, and polyads. GISH was used in the interspecific hybrids and pairing between homeologous chromosomes, and bivalent and tetravalent formation were observed. From this study, we could conclude that hybrid genotypes are fertile and pollen grains are viable and can be used in breeding programs. We also hypothesize that interspecific hybrid genotypes of Passiflora can be obtained with regular meiosis, which could be viable and fertile.

show abstract

Morphological and cytogenetic characterization of new ornamental Passiflora hybrids (P. ‘Vivis’ and P. ‘Jhovi’)

Cited by 4 publications

References 22 publications

Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

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