Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field

Gorski, R; Nowak-Terpiłowska, Agnieszka; Śledziński, Paweł; Baranowski, Mikołaj; Wosiński, Stanisław

doi:10.26444/aaem/118260

Cited by 12 publications

(9 citation statements)

References 54 publications

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“…The cells’ morphological changes were described using the proposed model [ 41 , 42 ]. Figure 3s shows the images of normal cell morphology before irradiation.…”

Section: Resultsmentioning

confidence: 99%

Effect of femtosecond laser interaction with human fibroblasts: a preliminary study

2023

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In in vitro methods and cell culture models, femtosecond (fs) laser interaction has been employed to assess its effect on the proliferation and morphology of human skin fibroblasts. We cultured a primary human skin fibroblast cell line on a glass plate, passages 17–23. The cells were irradiated with a 90-fs laser at a wavelength of 800 nm and a repetition rate of 82 MHz. The target received an average power of 320 mW for 5, 20, and 100 s, corresponding to the radiation exposures of 22.6, 90.6, and 452.9 J/cm2, respectively. Using a laser scanning microscopy technique, the photon densities were measured to be 6.4 × 1018, 2.6 × 1019, and 1.3 × 1020 photons/cm2 in a spot area of 0.07 cm2; the recorded spectra were obtained from the laser interaction after 0.00, 1.00, 25.00, and 45.00 h. The cell count and morphological changes showed that the cultured cells were affected by laser irradiation under photon stress; some fibroblasts were killed, while others were injured and survived. We discovered evidence of the formation of several coenzyme compounds, such as flavin (500–600 nm), lipopigments (600–750 nm), and porphyrin (500–700 nm). This study is motivated by the future development of a novel, ultra-short fs laser system and the need to develop a basic in vitro understanding of photon–human cell interaction. The cell proliferation indicated that cells are partly killed or wounded. The exposure of fibroblasts to fs laser fluence up to 450 J/cm2 accelerates cell growth of the viable residual cell.

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“…The cells’ morphological changes were described using the proposed model [ 41 , 42 ]. Figure 3s shows the images of normal cell morphology before irradiation.…”

Section: Resultsmentioning

confidence: 99%

Effect of femtosecond laser interaction with human fibroblasts: a preliminary study

2023

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“…It is estimated that about 5 out of 100 000 people suffer from it annually. The average survival time of a patient diagnosed with glioblastoma, using current treatment standards, is just over 15 months from diagnosis, and median survival is estimated at 11 -12 months [33,34]. Research shows that only 3 -8% of patients live longer than three years [35].…”

Section: Discussionmentioning

confidence: 99%

Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG)

Nowak-Terpiłowska,

Górski,

Marszałek

et al. 2023

Ann Agric Environ Med.

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Introduction.Mobile phones and Wi-Fi are the most commonly used forms of telecommunications. Initiated with the first generation, the mobile telephony is currently in its fifth generation without being screened extensively for any biological effects that it may have on humans or on animals. Some studies indicate that high frequency electromagnetic radiation emitted by mobile phone and Wi-Fi connection can have a negative effect upon human health, and can cause cancer, including brain tumour.Objective. The aim of the study was to investigate the influence of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on the proliferation and morphology of normal (human embryonic kidney cell line Hek-293) and cancer cells (glioblastoma cell line U-118 MG). Materials and method. The cell cultures were incubated in RF-EMF at the frequency of 2.4 GHz, with or without dielectric screen, for 24, 48 and 72h. In order to analyse the influence of the electromagnetic field on cell lines, Cytotoxicity test Cell Counting Kit-8 was performed. To protect cells against emission of the electromagnetic field, a dielectric screen was used. Results. It was found that 2.4 GHz RF electromagnetic field exposure caused a significant decrease in viability of U-118 MG and Hek-293 cells. The impact of the electromagnetic field was strongest in the case of cancer cells, and the decrease in their survival was much greater compared to the healthy (normal) cells of the Hek-293 line. Conclusions. Results of the study indicate that using a radio frequency electromagnetic field (2.4 GHz) has a clearly negative effect on the metabolic activity of glioblastoma cells. RF-EMF has much less impact on reducing the viability of normal cells (Hek -293) than cancer cells.

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“…One study exposed human fibroblasts and prostate cancer cells to RF-EM (2.5 GHz, the same as cell phones and WIFI/ Bluetooth) for 24, 48, and 72 hours. 20 Fibroblast viability was decreased at all time points and there was a resultant increase in prostate cancer cell growth. A 2020 study comprehensively evaluated the effect of RF-EM on human fibroblasts using high-throughput next-generation sequencing and biomarker analysis.…”

Section: Radiofrequency Electromagnetic Radiation Affects Metabolism ...mentioning

confidence: 90%

“…Data are mixed regarding the effects of RF-EM on human fibroblasts. 20,21 Radiofrequency electromagnetic radiation may affect gene expression in fibroblasts and alter fibroblast viability and metabolism. This could have deleterious effects on our dermis, including resilience from of stress, wound healing, plumpness of our dermis, and distribution of collagen.…”

Section: Radiofrequency Electromagnetic Radiation Affects Metabolism ...mentioning

confidence: 99%

Is the Technology Era Aging You? A Review of the Physiologic and Psychologic Toll of Technology Use

McKesey

Fabi

2022

Dermatol Surg

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BACKGROUND Technology use is at an all-time high and its potential impact on psychological and physiologic health should be explored. OBJECTIVE The objective of this narrative review was to identify the role of technology use on health and well-being. MATERIALS AND METHODS Authors performed a review of PubMed and publications of the World Health Organization, Department of Defense, and Centers for Disease Control and Prevention to determine the impact of technology regarding electromagnetic radiation (EM), posture and mobility, sleep disturbance, and psychological stress and well-being. RESULTS Studies on the impact of EM were conflicting, with about 45% reporting negative consequences and 55% reporting no effect. Radiofrequency EM (RF-EM) may more significantly affect fibroblasts and immature cells. Device use was implicated in worsening cognitive focus, imbalance, and sleep. Social media use affects self-esteem and mental health and is associated with up to 33% presence of addiction. Effects seem to be dose related and more pronounced in younger ages. CONCLUSION Technology use significantly affects sleep, mental health, and cognitive function. Seeking psychological help, limiting social media use, and reducing use before sleep may partially mitigate these effects. The impact of EM is undetermined, but the WHO lists RF-EM as a potential carcinogen.

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Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field

Abstract: Nowak-Terpiłowska A, Górski R, Śledziński P, Baranowski M, Wosiński S. Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field. Ann Agric Environ Med.

Cited by 12 publications

References 54 publications

Effect of femtosecond laser interaction with human fibroblasts: a preliminary study

Effect of femtosecond laser interaction with human fibroblasts: a preliminary study

Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG)

Is the Technology Era Aging You? A Review of the Physiologic and Psychologic Toll of Technology Use

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