Morphological and functional changes after portal vein occlusion in rats

Abstract

We studied the morphological and functional changes after ligation or embolization of a portal vein branch that perfused the median and the left lateral lobes of the liver in rats. After surgery, regenerative hypertrophy in the non-occluded liver lobes and atrophy in the occluded liver lobes were seen. In the non-occluded liver lobes, mitosis of the hepatocytes was seen most frequently on postoperative day 2. The density of the Kupffer cells after postoperative day 2 was about twice that in intact rats. In the occluded liver lobe, apoptosis of the hepatocytes was seen most frequently on the postoperative day 4, but the density and the phagocytic activity of Kupffer cell were reduced. There was no significant difference in hepatic parenchymal function such as K1CG and the blood levels of antithrombin III between rats on postoperative day 7 and intact rats. On the other hand, liver regeneration was presented even in rats with cirrhosis.However, the DNA synthesis and ODC activity in cirrhotic rats were lower than those in normal rats. Workshop w1The evaluation of postoperative early complications and long-term nutrition after pancreatoduodenectomy Y. Nakamoto, T.K ajiwara,H. Meguro,T. Tani, Y. Konisi, K. Yanagihasi ,T.Hashimoto,S .Fujiie Dept. of Surgery, Kobe city general hospital 155 pancreatoduodenectomy(PD) was investigated in our situation from 1981 Aug. to 1995 Dec.. PD-II : 58 cases, PD-Ill : 50 cases, PpPD-III : 42 cases, others : 5 cases. We evaluated early complications and long-term nutrition after the surgery. Among these three procedures there was no significant difference in leakage and heavy complications ( acute renal failure, MOF, DIC). The ratio of postoperative intraperitoneal bleeding was significantly less after PpPD-III than after others. The incidence of peptic ulcer was 1 case in each procedure. The incidence of fatty liver was 3 cases in PD-II, 5 cases in PpPD-III. In 6months weight gain was better after PpPD-III, but there was no statistically significant differences. In 12 months, weight gain was significantly greater after PpPD-1II. In 6, 12 months gains of serum albumin, it was better after PpPD-lll,but there was no statistically significant differences. In PD-III, PpPD-1II the passage of food is physical, and in PpPD-III postoperative long term nutrition is superior. We recommend this procedure. W2Function of the remnant pancreas after pancreatoduodenectomy (PD) -short term and long term-H.Taoka, TJ-Iigasiguchi, H.Yokoi, Y.Ogura, T.Noguchi, and Y. Kawarada 1st Dept. of Surg., Mie Univ., Mie, JapanThe purpose of this study was to assess the pathophysiology of the remnant pancreas after PD, and PpPD or DpPHR (pPD below) . The subjects of this study were 67 cases who underwent PD and 12 cases who underwent pPD during the past 10years. Patients with diseases in which the main lesion was in the pancreatic parenchyma (e.g., pancreatic carcinoma and chronic pancreatitis) were classified in group A and patients with in which the main lesion was outside the pancreatic parenchyma ( e.g., carcinom...

Alterations in hepatic lobar function in regenerating rat liver

“…This mass loss was fully compensated by the rapid weight gain observable in case of the PVNL lobes; consequently, the total liver weight remained constant. The weight gain in PVNL lobes reached a plateau phase between the fifth and seventh days at approximately 329% of the baseline, which finding was consistent with previously published data [5,21,22]. However, the specific alterations in hepatic lobar structure have not been assessed so far.…”

“…On the seventh day, the liver lobules of the PVL lobes became considerably smaller and were characterized by contracted portal triads and narrowed sinusoids. Earlier, Tanaka et al [21] and Yamasaki et al [33] demonstrated that arterialization of the PVL lobes (absence of portal nutrients, hormones, and other mediators) led to the partial capillarization of liver sinusoids, characterized by a decreased number of fenestrae and porosity, as well as by partial development of the basement membrane. These alterations can reduce the diffusion of protein-bound substances such as ICG and are most likely responsible for the permanent functional impairment observed by us in the PVL lobes.…”

Triggered Liver Regeneration: From Experimental Model to Clinical Implications

“…However, the lobules have become smaller (the portal areas lie closer to each other). At the edge of the shrunken lobules, contracted portal triads can be found together with a fibrotic thickened portal vein, a collapsed bile duct and an enlarged hepatic artery [39,40]. Furthermore, the sinusoidal spaces are narrowed, and the number of fenestrations per endothelial cell decreases with the partial formation of basement membrane [41].…”

“…According to the most widely used laboratory blood tests (prothrombin time and antithrombin III, albumin, bilirubin and transaminase levels), liver function remains stable after PVO [40,64,67]. However, the blood concentration of liver-related biochemical substances is dependent on many factors (blood flow, volume of distribution, availability or elimination).…”

“…Based on the experiments by Tanaka et al [40] and Furrer et al [63], conducted in rats, PVL triggers an earlier and greater hypertrophy than PVE. According to these experiments, PVE induces a massive inflammatory reaction surrounding the embolization material (foreign-body reaction).…”