Morphological and genetical changes of endothelial progenitor cells after in - vitro conversion into photoreceptors

Shi, Qiang; Alsaeedi, Hiba Amer; Cheng, Yannan; Yang, Hao; Li, Tong; Kumar, Suresh; Higuchi, Akon; Alarfaj, Abdullah A.; Munisvaradass, Rusheni; Ling, Mok Pooi; Pei, Cheng

doi:10.1016/j.jphotobiol.2018.04.003

Cited by 6 publications

(8 citation statements)

References 21 publications

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“…The clinical use of these cell sources can thus bring about some problems including complications of microsurgical procedures, limited number of pluripotent retinal stem cells, differentiation into photoreceptors, formation of an abnormal cell orientation, and rejection by host immune systems [2]. A number of investigations have further shown the multilineage potential of mesenchymal stem cells (MSCs) into neural ones [9]. These findings have also prompted researchers to attempt their induction into photoreceptors for the treatment of degenerative retinal diseases [1].…”

Section: Introductionmentioning

confidence: 99%

“…In this domain, the bone marrow is acknowledged as an ideal source of pluripotent stem cells that has a highly undifferentiated and self-renewing potential. Because of its autologous characteristics, relative ease of isolation, and less controversial nature, this pool of pluripotent stem cells remains as a forerunner of the cells of choice in the treatment of diseases via cell-based therapies [9]. There have been also some reports about differentiation of bone marrow mesenchymal stem cells (BMSCs) into photoreceptors through inducing MSCs cultured with photoreceptors and conditioned medium in rat models [1, 10].…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Mahmoudian-Sani

Forouzanfar

Asgharzade

et al. 2019

Journal of Ophthalmology

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Retinal degeneration is considered as a condition ensued by different blinding disorders such as retinitis pigmentosa, age-related macular degeneration, and diabetic retinopathy, which can cause loss of photoreceptor cells and also lead to significant vision deficiencies. Although there is no efficient treatment in this domain, transplantation of stem cells has been regarded as a therapeutic approach for retinal degeneration. Thus, the purpose of this study was to analyze the potential of human bone marrow-derived mesenchymal stem cells (hBMSCs) to differentiate into photoreceptor cells via transfection of microRNA (miRNA) in vitro for regenerative medicine purposes. To this end, miR-183/96/182 cluster was transfected into hBMSCs; then, qRT-PCR was performed to measure the expression levels of miR-183/96/182 cluster and some retina-specific neuronal genes such as OTX2, NRL, PKCα, and recoverin. CRX and rhodopsin (RHO) levels were also measured through qRT-PCR and immunocytochemistry, and subsequently, cellular change morphology was detected. The findings showed no changes in the morphology of the given cells, and the expression of the neuroretinal genes such as OTX2, NRL, and PKCα. Moreover, recoverin was upregulated upon miR-183/-96/-182 overexpression in cultured hBMSCs. Ectopic overexpression of the miR-183 cluster could further increase the expression of CRX and RHO at the messenger RNA (mRNA) and protein levels. Furthermore, the data indicated that the miR-183 cluster could serve as a crucial function in photoreceptor cell differentiation. In fact, miRNAs could be assumed as potential targets to exploit silent neuronal differentiation. Ultimately, it was suggested that in vitro overexpression of miR-183 cluster could trigger reprogramming of the hBMSCs to retinal neuron fate, especially photoreceptor cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Mahmoudian-Sani

Forouzanfar

Asgharzade

et al. 2019

Journal of Ophthalmology

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“…Taurine has been introduced as one of the organic amino acids, which regulate the cells' volumes 13 . The neural as well as nonneural ocular tissues possess taurine and massive quantity of this substance exists in retina, vireous, lens, iris ciliary body and cornea 14 . Also photoreceptors have been identified has the richest section with taurine in comparison to other retinal cell 14 .…”

Section: Discussionmentioning

confidence: 99%

“…The neural as well as nonneural ocular tissues possess taurine and massive quantity of this substance exists in retina, vireous, lens, iris ciliary body and cornea 14 . Also photoreceptors have been identified has the richest section with taurine in comparison to other retinal cell 14 . Moreover, taurine deficiency results in the visual deficiencies result that photoreceptor degeneration as well as the RGC losses 15 .…”

Section: Discussionmentioning

confidence: 99%

Retinoic acid and taurine enhance differentiation of the human bone marrow stem cells into cone photoreceptor cells and retinal ganglion cells

Forouzanfar

Soleimannejad

Soltani

et al. 2021

J of Cellular Biochemistry

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Degeneration and apoptotic death of the photoreceptor cell‐layer of retina are a major cause of irreversible blindness in the development era. The stem cell replacement therapy is one of the strategies for the retinal repairing. In addition, exogenous signals critically contribute to the direction of lineage decisions that causes the fate‐restricted photoreceptor progenitors from stem cell progeny in culture. It has been found that epidermal growth factor (EGF), taurine, and retinoic acid (RA) initially act in the instructive as well as lineage‐restricted way in the progenitor lineage for producing neuroretinal cells or photoreceptor like cells from stem cell. The study aims to investigate the effect of RA and taurine in differentiation of the human bone marrow stem cell into cone photoreceptors cells and retinal ganglion cells. Mesenchymal stem cell was derived from human bone marrow of the term delivery. Therefore, the cultured cells have been treated with Dulbecco's modified Eagle's medium (DMEM)/high glucose (H+). After the four‐cell passage, basal medium was replaced with DMEM/F12 complemented with 50 μmol/L taurine, RA (1 µM) and EGF (1 µg/ml). Subsequently cellular change morphology was detected following 7 and 14 days. Then, gene expression of neuroretinal and photoreceptor cell biomarkers (CRX, OTX2, PKC‐α, recoverin, and Rho) were examined by quantitative polymerase chain reaction (Q‐PCR). Also, cells were cultured, fixed, and then immunocytochemical analyzed. Primary antibodies included CRX and Rho. Cellular morphology demonstrated spindle elongated morphology. Taurine alone and combination of RA upregulate neuroretinal and photoreceptor cell biomarkers in messenger RNA and protein levels but along with EGF have not significant effect. Our data showed that taurine combination with RA can differentiate bone marrow mesenchymal stem cells into neuroretinal or photoreceptor like cells in vitro that can offer an attractive treatment ground for transplantation in the cell‐replacement therapy for some forms of the retinal degeneration.

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“…To perform daily life activities vision is essential due to degenerative eye disorders irreversible vision loss [35]. Among cellbased approaches for the restoration of vision photoreceptors replacement is one of them in regenerative medicine, it is the stem cell-based therapy [36]. Retinal pigment epithelium is transplanted into the patient along with fetal retinas for the improvement of vision [37].…”

Section: Introductionmentioning

confidence: 99%

Restoring Vision by Regenerative Medicine Using Stem Cell: A Review

Rk¹,

Ibrahim²,

Shafiq³

et al. 2018

J Biomol Res Ther

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Restoring vision is the most important center of attention of our research program, On the other hand, to attain our objective and convey the preeminent eminence of stem cell therapy; we have to understand the fundamental biology of stem cells. (RPE) Retinal Pigmented epithelium layer degenerates and contributing to a succession of actions resulting in visual impairments. Regenerative medicine holds promise for the spanning almost for all body system and the eye is an ideal organ to use in research because it is the only part of nervous system that is the visible and easily accessible. This review outlines advances in therapy and the main spotlight for the improvement and advance of cell therapies that are being confronted today.

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Morphological and genetical changes of endothelial progenitor cells after in - vitro conversion into photoreceptors

Cited by 6 publications

References 21 publications

Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Retinoic acid and taurine enhance differentiation of the human bone marrow stem cells into cone photoreceptor cells and retinal ganglion cells

Restoring Vision by Regenerative Medicine Using Stem Cell: A Review

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